Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2006-02
Add to Favorite Get Latest Update

Cloning and hpRNAi Vector Construction of Brassica napus Pyrophosphate-dependent Phosphofructokinase (PFK)

Wang Chenggang Zhang Juhong Xie Yu Liu Youqi Huang Bangquan*Lab of Plant Genetics and Breeding, College of Life Science, Hubei University, Wuhan, 430062  
Pyrophosphate-dependent phosphofructokinase (PFK) plays an important role in the EMP pathway in rapeseed (Brassica napus L.). Firstly we constructed plant expression vector 2300-nap harboring seed-specific napin promoter and nos terminator, and plant expression vector 2300-35S harboring constitutive 35S promoter and nos terminator. PFK gene fragment was PCR-amplified from B. napus genomic DNA and was used as template to amplify a smaller fragment. These two PFK fragments were ligated in opposite directions into plant expression vector 2300-nap and 2300-35S respectively to produce hpRNAi vector hp-PFK-nap and hp-PFK-35S. This is the first step to increase oil content in rapeseed using RNA interference.
【CateGory Index】: S565.4;Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 4 Hits
1 ZHANG Gong1,LIU Li-feng1,ZHOU Wei2,WANG Hai-guang2,MA Zhi-ying1(1.College of Agronomy,Agricultural University of Hebei,Baoding 071001,China;2.Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture,China Agricultural University,Beijing 100094,China);Construction of Expression Vector for Anti Stripe Virus of Rice by RNA Interference[J];Acta Agriculturae Boreali-Sinica;2008-04
2 WANG Cheng-gang,WANG Wen-yan,XIE Yu,HUANG Bang-quan (1 School of Life Science,Hubei University,Wuhan 430062,China;2 Wuhan Bioengineering Institute,Wuhan 430415,China);Cloning and hpRNA vector construction for fad2 genes in Brassica napus and Lesquerella fendleri[J];Journal of Hubei University(Natural Science);2007-01
3 WANG Cheng-gang,LI Chang-chun,XIE Yu,LIU You-qi,HUANG Bang-quan (School of Life Science,Hubei University,Wuhan 430062,China);Cloning and hpRNA vector construction for Brassica napus glycolate oxidase gene(GO)[J];Journal of Hubei University(Natural Science);2007-03
4 WANG Cheng-gang,XIE Yu,LI Chang-chun,LIU You-qi,HUANG Bang-quan(Lab of Plant Genetics and Breeding,College of Life Science,Hubei University,Wuhan 430062,China);Cloning and hpRNA vector construction for genes of cruciferin,napin,oleosin and PEPC in Brassica napus L.[J];Chinese Journal of Oil Crop Sciences;2006-03
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Li Jia; Shen Bingzhang; Han Jixiang; Gan Li (Huazhong Agricultural University, Wuhan 430070);A EFFECTIVE PROCEDURE FOR EXTRACTING TOTAL DNA IN RAPE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1994-05
2 Wu Yanyou;Mu Zhonglin;Luo Peng;APPLICATION OF SODIUM IODOACETATE IN THE MUTAGENESIS[J];JOURNAL OF SICHUAN UNIVERSITY (NATURAL SCIENCE EDITION);1996-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Tang Tianze 1Xiao Xiaoyu 2 Shui Hongxia 1 1 Mianyang Institute of Agricultural Sciences, Mianyang, 621023; 2 Sichuan Provincial Seed Station, Chengdu, 610041 The authors who contribute equally;Comparison of Hybrid Rapeseed Purity Based on SSR Markers and Morphological Phenotyping[J];Molecular Plant Breeding;2008-02
2 ZHANG Shu-fen~(1,2),FU Ting-dong~1,LI Yuan-yuan~1,ZHU Jia-cheng~2,WANG Jian-ping~2,MA Chao-zhi~1(1.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China,2.Cotton and Oil Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China);Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Brassica napus L[J];Acta Agriculturae Boreali-Sinica;2006-01
3 LIU Zhi-wen,WANG Ying,LIU Xue-ping, FU Ting-dong XUE Yong-chang,TUJin-xing,MA Chao-zhi (College of Bio & Food Technology, Dalian Institute of Light Industry,Dalian 116034,China; National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070,China);The Effects Analysis of Molecular Marter-assisted Selection in Yellow-seeded Brassica napus[J];Acta Agriculturae Boreali-Sinica;2006-02
4 WANG Cheng-gang,WANG Wen-yan,XIE Yu,HUANG Bang-quan (1 School of Life Science,Hubei University,Wuhan 430062,China;2 Wuhan Bioengineering Institute,Wuhan 430415,China);Cloning and hpRNA vector construction for fad2 genes in Brassica napus and Lesquerella fendleri[J];Journal of Hubei University(Natural Science);2007-01
5 WANG Cheng-gang,LI Chang-chun,XIE Yu,LIU You-qi,HUANG Bang-quan (School of Life Science,Hubei University,Wuhan 430062,China);Cloning and hpRNA vector construction for Brassica napus glycolate oxidase gene(GO)[J];Journal of Hubei University(Natural Science);2007-03
6 LI Chang-chun,QIAO Fu-xing,YAN Xiao-feng,DING Quan-ru,HUANG Bang-quan(School of Life Science,Hubei University,Wuhan 430062,China);Cloning and hpRNAi vector construction for gene of thiohydroximate S-glucosyltransferase and genetic transformation in Brassica napus[J];Journal of Hubei University(Natural Science);2008-03
7 ZHENG Zhuo1,2b,LI Jian2a,LI Xun2b,GUAN Chun-yun2b(1.College of Bioscience,Jinggangshan University,Jian,Jiangxi 343009,China;2.a.College of Bioscience and Technology;b.Oil Crops Research Institute,HNAU,Changsha 410128,China);Compatibility and Molecular Identification of Intergenetic Hybrids between the Xinjiang Wild Rape(Brassica arvensis L.) and Brassica napus L.[J];Journal of Hunan Agricultural University(Natural Sciences);2006-05
8 GAO Yu-qian~1, ZHANG Shu-fen~(2), ZHU Jia-cheng~2, ZHANG Shi-min~1, QI Yuan-cheng~1(1.College of Life Science, Henan Agricultural University, Zhengzhou 450002, China; 2.Cotton and Oil Crops Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China);Selection of Polymorphism of Brassica napus L R4 and 22B by AFLP Analysis[J];Journal of Henan Agricultural Sciences;2005-08
9 ZHU Jia-cheng1,GAO Yu-qian2,ZHANG Shu-fen1,WEN Yan-cheng1,WANG Jian-ping1(1.Indurstrial Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China);Mapping for Fertility Restorer Genes of Cytoplasmic Male Sterility in Brassica napus L.[J];Journal of Henan Agricultural Sciences;2008-10
10 Tu Jingxing. Zheng Yonglian Fu Tingdong (Huazhong Agricultural University , Wuhan 430070);RAPD MARKS LINKED TO GENETIC MALE STERILE GENE OF RAPESEED[J];Journal Huazhong (Central China) Agricultural University;1997-02
China Proceedings of conference Full-text Database 1 Hits
1 HAN Yi-qiang,DUAN Hai-yan,WANG Yun-hua,MENG Jin-ling ,XU Fang-sen, YE Xiang-sheng.ZHAO Zhu-qing (Nrtional Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China ;Laboratory of Microelement, Huazhong Agriculture University,Wuhan 430070, China);Identification of Phosphorus efficiency germplasm and QTL mapping in Brassica napus[A];[C];2004
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZOU Lin,YE Di-qing,ZHANG Peng-hui(1.Department of Laboratory Medicine,the First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China;2.Faculty of Laboratory Medicine,Chongqing University of Medical Sciences,Chongqing 400016,China);The construction and application of siRNA-DNA expression vector[J];Chongqing Medical Journal;2005-09
2 Zhu Longfu Zhang Xianlong(National Key Laboratory of Plant Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China);RNAi and Its Application in Plant Genetic Improvement[J];Journal of Huazhong Agricultural;2004-04
3 Li Jia; Shen Bingzhang; Han Jixiang; Gan Li (Huazhong Agricultural University, Wuhan 430070);A EFFECTIVE PROCEDURE FOR EXTRACTING TOTAL DNA IN RAPE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1994-05
4 Chen Jinqing; Lang Chunxiu; Hu Zhanghua Liu Zhihong Huang Ruizhi(Institute for Application of Atomic Energy, Zhejiang Academy of Agricultural Sciences, Hangzhou 3l0O21);Antisense PEP Gene Regulates to Ratio of Protein and Lipid Content in Brassica Napus Seeds[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1999-04
5 Shi Dongqiao, Zhou Yihua, Hu Zanmin, Zhang Lihua, Liu Guizhen, Chen Zhenghua (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101);Introduction of trans Desaturase Gene into Brassica napus L. via ParticleBombardment and Obtaining of Transgenic Plants[J];Journal of Agricultural Biotechnology;2001-04
6 Yan FeiCheng Zhuomin(Institute of plant protection, CAAS, SKLBPI, Beijing100094);RNA Interference and Plant Anti-virus[J];Biotechnology Information;2005-04
7 LI Xiao Ping DENG Nan MA Yuan Yuan LI Peng Li WANG Yong ZHANG Ren WANG Ning Ning (Plant Biology and Ecology Department, Nan Kai university, Tianjin 300071; Department of Biological Sciences, University of Wollongong, NSW2522 Australia);CONSTRUCTION OF RNAi BINARY VECTOR OF SOYBEAN RECEPTOR-LIKE KINASE GENE (rlpk2) AND ITS SOYBEAN TRANSFORMATION[J];Journal of Molecular Cell Biology;2006-01
8 Meng Qingwei Zhao Shijie Xu Changcheng Zou Qi(Shandong Agricultural University, Taian 271018);Photoinhibition of Photosynthesis and Protective Effect of Photorespiration in Winter Wheat Leaves under Field Conditions[J];Acta Agronomica Sinica;1996-04
9 Peng Zimo (Life and Environment Sciences Institute,Xinjiang Normal University,Urumqi,830054)\;;Physiological Meaning and Control of Plant Photorespiration[J];Journal of Xingjiang Normal University(Natural Sciences Edition);2001-02
10 SUN Dai-zhen 1, JIANG Ling 1, ZHANG Ying-xin 1, CHENG Xia-nian 1, WANG Chun-ming 1, ZHAI Hu-qu 2, WAN Jian-min 1,2,*(1 State Key Laboratory of Crop Genetics and Germplasm Enhancement; Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China; 2Chinese Academy of Agricultural Sciences, Beijing 100081, China;);Resistance to Rice Stripe in Eight Rice Varieties[J];Chinese Journal of Rice Science;2006-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 Zhang Yong 1,2 Fu Shaohong 1,2 Zhang Ruquan 2 Niu Yingze 1 1 Rapeseed Research Center, Sichuan Agricultural University, Yaan, 625014; 2 Branch Center of The National Rapeseed Improvement Center in Chengdu, Sichuan, Institute of Crop Science, Chengdu Academy of Agricultural and Forestry Sciences, Wenjiang, 611130;Cloning of the PEPC Gene and Construction of a Seed-specific ihpRNA Expression Vector in Brassica napus L.[J];Molecular Plant Breeding;2008-04
2 LIU Zhi-wen SHAN Lian-min HAN Xu MAO Wei(College of Biology and Food Technology,Dalian Polytechnic University,Dalian 116034,China);Application and Progress of the Modern Biotechnology in the Rapeseed Genetic Improvement[J];Journal of Huazhong Agricultural University;2007-06
3 Li Changchun1,Qiao Fuxing2(1.College of Life Science and Technology,Xiaogan University,Xiaogan,Hubei 432000,China;2.School of Life Science,Hubei University,Wuhan,Hubei 430062,China);Cloning and hpRNAi Vector Construction for Genes of Thiohydroximate S-glucosyltransferase in Crambe abyssinica[J];Journal of Xiaogan University;2008-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Tang Zhang--cheng; Wu Ya-hua; Wang Yu--qi; Wang Hong-chun Shanghai Institute of Plant Physiology, Academia Sinica;FREE PROLINE AND DROUGHT RESISTANCE IN SORGHUM SEEDLINGS[J];Plant Physiology Communications;1986-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved