An ILP Marker Close Linking with the Tentative Novel F_1 Pollen Sterile Locus in Cultivated Rice
Wu Haibin1,2 Zhu Rucai1 Li Di1 Zhao Degang2* Bai Yangnian1*1 Hainan Institute of Tropical Agricultural Resources, Sanya, 572025; 2 Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, Guiyang, 550025
The F2 population containing 500 individuals was developed using two cultivated rice germplasms (Oryza sativa L.), HITAR005 as female parent from HITAR and IRGC20509 as male parent from IRRI, and the ILP (intron length polymorphism) markers were employed to genotype the 117 individuals of the F2 population. RI01954, an ILP marker mapped in the 3rd chromosome, was found that its homozygous locus for male parent were completely absent in the 117 individuals and the ratio of RI01954 locus between homozygous of female parent and heterozygous was 1:1 (X2_~CC=0.90, X2_~CCX20.05), which showed obviously distorted segregation in the F2 population. The sequence conferring RI01954 was deeply analyzed that it is unknown functional transcript factor containing three introns. There is 24bp deletion of the 3rd intron in Japonica Nipponbare comparing with Indica 9311. Analysis of PCR products amplified by primers of RI01954 showed that the bands for male parent disappeared were identical to Japonica Nipponbare. Combining the result of pollen fertility analysis in the F2 population, it was primary concluded that RI01954 marker closely linked to the phenotype of the F1 pollen sterility locus and the genetic distances between them was estimated less than 0.5cM. And also it suggested that the locus linked with RI01954 was a novel F1 pollen sterile locus in the cultivated rice.
【CateGory Index】： S511.2