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《Molecular Plant Breeding》 2006-03
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DNA Fingerprinting Technology and its Application in Crop Germplasm Resources

Wang ZhonghuaInstitute of Biotechnology, Zhejiang Wanli University, Ningbo, 315100  
DNA fingerprinting technologies have been rapidly developed with the appearance of kinds of new DNA molecular markers recently. This paper briefly reviewed the basic principle, advantages and disadvantages of several DNA fingerprinting technology, such as restriction fragment polymorphism(RFLP), random amplified polymorphic DNA (RAPD), amplified fragmentlength polymorphism (AFLP), simple sequence repeat (SSR), inter-simple sequence repeat (ISSR) and single nucleotide polymorphisms (SNPs) etc. And its application in crops germplasm, including the identification of crop varieties, the examination of seed purity, the study of relationship and classification of crop varieties and the exact protection of crop varieties patents. In addition, the problems existing in the application of DNA fingerprinting technology and the ways to solve them were also briefly discussed, such as the contradiction between DNA extraction methods and application of breeding populations, limited application due to complicated relatively technique of many DNA fingerprinting, etc.
【CateGory Index】: S32;Q943.2
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Chinese Journal Full-text Database 3 Hits
1 WANG Zhong-hua1,2 LI Li-jun1 WANG Zhuo-yi1(1.Institute of Biotechnology,Zhejiang Wanli University,Ningbo,Zhejiang 315100;2.Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou,Zhejiang 310029);COMPARATIVE STUDY OF EXTRACTION METHOD FOR GENOMIC DNA IN MAT RUSH[J];Journal of Nuclear Agricultural Sciences;2008-02
2 WANG Zhonghua, DONG Xizheng, QIAN Guoying (Institute of Biotechnology, Zhejiang Wanli University, Ningbo 315100, China);Application in Genetic Diversity of Aquatic of DNA Molecular Markers[J];Bulletin of Science and Technology;2008-05
3 WANG Wei1,2,YANG Wen-peng2,3,DAI Bao-wei1,GUAN Qi1,2;DNA Fingerprinting Technology and Its Application to Genetics and Breeding in Maize[J];Seed;2008-01
China Proceedings of conference Full-text Database 1 Hits
1 ;Establishment of DNA Fingerprints on Non-Heading Chinese Cabbage Hybrids by RAPD,ISSR and SRAP[A];[C];2008
Chinese Journal Full-text Database 10 Hits
1 ZHANG Li rong, XU Da qing, LIU Da qun (College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China);SSR marker, ISSR marker and their application to plant genetics and breeding[J];Journal of Agricultural University of Hebei;2002-01
2 Chen Liang Yang Yajun Yu Fulian Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture/ Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008;Genetic Diversity, Relationship and Molecular Discrimination of elite Tea Germplasms [Camellia sinensis (L.) O. Kuntze] Revealed by RAPD Markers[J];Molecular Plant Breeding;2004-03
3 DU Dao-lin 1,SU Jie 1,ZHOU Peng 2,ZHENG Xue-qin 2 (1.Department of Biology,Hainan Normal University,Haikou 571158; 2.National Key Biotechnology Laboratory for Tropical Crops,Haikou 571101,China);RAPD technique and its application in plant germplasm and genetic breeding[J];Journal of Hainan Normal University(Natural Science);2002-Z1
4 He Yuqing; Zhang Yu; Sun Mei; He Guangchun; Wang Songwen; Zhu Yingguo ( College of Life Science, Wuhan University, Wuhan 430072; The Department of Zoology, Hong Kong University; The Department of Agronomy, Tianin Agricultural College, Tianjin 3003ll );Studies on the Relationship between Cultivated and Wild Rice Using ISSR Markers[J];Journal of Agricultural Biotechnology;2001-02
5 LI Chang-you (College of Life Science,Jilin Normal University,Siping 136000,China);The Advances and Application of DNA Fingerprint[J];Jilin Normal University Journal (Natural Science Edition);2004-02
6 WANG Zhong-Hua (Institute of Biotechnology, Zhejiang Wanli University, Ningbo 315100, China);Marker-assisted selection, molecular mapping and cloning of the rice blast resistance genes[J];Chinese Bulletin of Life Sciences;2005-02
7 ZOU Yu Ping, GE Song Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093;A novel molecular marker-SNPs and its application[J];Chinese Biodiversity;2003-05
8 Qiu Lijuan Randall L. Nelson Lila O. VodkinInstitute of Crop Germplasm Resources, CAAS, Beijing 100081, China; USDA Soybean Germplasm Collection, Urbana, 1L 61801, USA, Department of Crop Science, University of Illinois, Urbana, IL 61801, USA;Evaluation of Soybean Germplasm with Random Amplification Polymorphic DNA(RAPD)Markers[J];Acta Agronomica Sinica;1997-04
10 QI Jian-Min 1,①,ZHOU Dong-Xin 2,WU Wei-Ren 1,LIN Li-Hui 1,FA NG Ping-Ping 1,WU Jian-Mei 1 (1.College of Crop science,Fujian Agriculture and Forestry University,Fuzhou,F ujian 350002,China; 2.Longyan Tobacco Branch Company,Longyan,Fujian 364000,China);The Application of RAPD Technology in Genetic Diversity Detection of Jute[J];Acta Genetica Sinica;2003-10
Chinese Journal Full-text Database 10 Hits
1 Li Zefu et al (Key Lab of Rice Genetics and Heredity, Anhui Academy of Agricultural Sciencs Hefei 230031);Types and Genetic Mechanisms of Plant Male Sterility[J];Journal of Anhui Agricultural Sciences;2000-06
2 WU Chang-fu (Lingnan Forest station of Xiuning County, Xiuning,Anhui 245421);Primary Study on Ecological Characteristics and Seedling Cultivation of Maire Yew[J];Journal of Anhui Agricultural Sciences;2005-10
3 ZHU Mei-xia et al(Agriculture Department,Hebei University of Engineering,Handan,Hebei 056038);Purity Identification of Cotton Varieties with SSR Technique[J];Journal of Anhui Agricultural Sciences;2005-11
4 LI Xiao-wei et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Investigations on the Community with Taxus mairei of Nature Reserve of Macaque in Jiyuan[J];Journal of Anhui Agricultural Sciences;2006-19
5 YAN Xing-chu et al (Institute of Oil Crops,Chinese Academy of Agricultural Sciences,Wuhan,Hubei 430062);The Status in Quo and Foreground of Exploiting Castor-oil Plant as Energy Sources[J];Journal of Anhui Agricultural Sciences;2007-34
6 WANG Lan 1* , HAN Yan-chuang 1* , PENG Yu-lu 1 , TENG Cai-zhu 1 , ZHOU Ming-quan 2** , HU Zhong-li 1, 2** , SONG Yun-chun 1 ,2 (1 Key Lab of the Ministry of Education for Plant Developmental Biology, Wuhan University, Wuhan 430072;2 Lotus center , Wuhan University, Wuhan 430072, China);Application of ISSR Technique to Genetic Research of Lotus Root[J];Amino Acids & Biotic Resources;2004-03
7 TENG Ji jun\ (Department of Neurology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China)\;;RELATIONSHIP BETWEEN VNTR POLYMORPHISM OF PLATELET GLYCOPROTEIN Ⅰb GENE AND CEREBRAL INFARCTION[J];Acta Acadimae Qingdao Umvesitatis;2003-03
8 CHANG Zhong Jie ZHOU Rong Jia YU Qi Xing (College of life sciences, Wuhan University, Wuhan 430072,China);GENETIC VARIATION OF TWO LOACH SPECIES REVEALED BY RAPD ANALYSIS[J];Acta Zoologica Sinica;2001-01
9 YANG Hui -Ping ① LI Li ① GUO Xi-Ming ①② (①Experimental Marine Biology Laboratory,Institute of Oceanology, The Chinese Academy of Sciences, Qingdao*"266071,Shandong,China) (②Haskin Shellfish Research Laboratory,Institute of Marine and Coa;PRELIMINARY STUDY ON INDUCING POLYPLOID IN JAPANESE SCALLOP (PATINOPECTEN YESSOENSIS) BY CYTOCHALASIN B[J];Acta Zoologica Sinica;2001-04
10 SONG Lin Sheng XIANG Jian Hai LI Chen Xi ZHOU Lin Hua LIU Rui Yu (Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,China) FAN Yong Jun (The Department of Biology, Nankai University,Tianjin 300071, China);STUDIES ON GENETIC VARIATION AND PHYLOGENETIC RELATIONSHIPS AMONG SIX SPECIES OF PENAEUS GENUS BY RAPD MARKERS[J];ACTA ZOOLOGICA SINICA;1998-03
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 ZHAN Qiu-wen1,LI Jie-qin1,WANG Bao-hua2,LI Yun-fei1(1.Anhui Science and Technology University,Fengyang 233100,China;2.National Key Laboratory of Crop Genetics & Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China);Establishment of DNA fingerprinting for 42 sorghum and sudangrass accessions and 2 sorghum-sudangrass hybrids[J];Acta Prataculturae Sinica;2008-06
2 KUANG Meng,YANG Wei-hua,XU Hong-xia,WANG Yan-qin,ZHOU Da-yun,FENG Xin-ai,WANG Jun-fang(Cotton Research Institute,CAAS;Supervision,Inspection and Test Center of Cotton Quality,Ministry of Agriculture,Anyang,Henan 455000,China);Research Progress of Molecular Marker Technology Applied in Cotton Variety Identification[J];Cotton Science;2009-04
3 XU Yan-xia,LI Xu-ye,WANG Xiao-chun,LI Xiao-bo(Animal Science Institute of Heilongjiang Province,Qiqihar,Heilongjiang 161005);Achievement and Development of Maize Breeding in China after the Establishment of PRC[J];Heilongjiang Agricultural Sciences;2009-06
4 Zhao Haiyan,Qu Yunfang,Huang Jinling(Dept of Agron,Shanxi Agricultural University,Taigu Shanxi 030801);Advances of SSR Marker Technology and Its Applications in Cotton Genetics and Breeding[J];Chinese Agricultural Science Bulletin;2009-22
China Proceedings of conference Full-text Database 3 Hits
1 KUANG Meng YANG Wei-hua XU Hong-xia WANG Yan-qin ZHOU Da-yun FENG Xin-ai WANG Jun-fang (Cotton Research Institute,CAAS;Supervision,Inspection and Test Center of Cotton Quality,Ministry of Agriculture,Anyang,Henan 455000,China);Research Progress of Molecular Marker Technology Applied in Cotton Variety Identification[A];[C];2008
2 WANG Jun fang YANG Wei-hua KUANG Meng XU Hong-xia WANG Yan-qin ZHOU Dayun FENG Xin-ai (Cotton Research Institute,Chinese Academy of Agricultural Science,An yang,Henan 455000,China);A Study of Cotton Variety DNA Fingerprinting Construction and Identification Techniques[A];[C];2008
3 XIE Zong-ming~1 DONG yong-mei~1 SU Jun-ji~2 (1.Center for Molecular Agrobiotechnology and Breeding,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China; 2.Xinjiang;Cotton Research Institute,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi Xinjiang 832000,China);Methods and Techniques Used to Test Genuiness and Purity of Seed of Hybrid Upland Cotton[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 JIA Yu yan 1, CHEN Hong 1,2 (1.College of Animal Science and Technology, Northwest Sci Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China; 2.Institute of Biotechnology, Xuzhou Normal University, Xuzhou, Jiangsu 221009, China);Molecular Genetic Marker of Single Nucleotide Polymorphism and its Application[J];Journal of Yellow Cattle Science;2003-01
2 CHEN Liang1,2, YAMAGUCHI Satoshi3, WANG Ping-sheng4, XU Mei4, SONG Wei-xi4, TONG Qi-qing1 (1.Department of Tea Science, Zhejiang University, Hangzhou 310029, China;2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;Genetic Polymorphism and Molecular Phylogeny Analysis of Section Thea Based on RAPD Markers[J];Journal of Tea Science;2002-01
3 LUO Jun-wu1,SHI Zhao-peng1,SHEN Cheng-wen1,LIU Chun-lin2, GONG Zhi-hua1,HUANG Yi-huan1 (1. Tea Science Department of Hunan Agricultural University; 2. Crop Gene Engineering Key Lab of Hunan Province,Changsha 410128, China);Studies on Genetic Relationships of Tea Cultivars [Camellia Sinensis (L.) O. Kuntze] by RAPD Analysis[J];Journal of Tea Science;2002-02
4 Chen Liang 1, Chen Daming 2, Gao Qikang 2, Yang Yajun 1, Yu Fulian 1 (1 Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008; 2 Zhejiang Agricultural University, Hangzhou 310029);Isolation and Appraisal of Genomic DNA From Tea Plant [ Camellia sinensis (L.)O.Kuntze][J];JOURNAL OF TEA SCIENCE;1997-02
5 Chen Shaojing;Yang Qingkai;Wang Jinling(Soybcan Institute,Northeast Agricultural Universith Harbin 150030 PRC) HuDongwei;Chen ShouyiCuiYunzhi (Genetics Institute,Academy of Seientific Sinica PRC)(Academy of Henan Agricultural Scicnce PRC);A RAPD STUDY ON THE GENETIC RELATION SHIP BETWEEN PARENTS AND OFFSPRINGS IN SOYBEAN BREEDING[J];JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY;1996-02
6 ZHOU Dong xin, QI Jian min,WU Wei ren, LI Wei ming (College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Studies on DNA extraction and establishment of RAPD reaction system in jute[J];Journal of Fujian Agricultural University(Natural Science);2001-03
7 Li Boing Ye Jian Ding Yan Ni Jintang Chu Xiaolan Institute of Forensic of public Security, Beijing;APPLICATION OF DNA TECHNIQUE IN RAPE AND PATERNITY DISPUTE CASES[J];Chinese Journal of Forensic Medicine;1989-04
8 Han Jicheng 2,3 Fang Xuanjun 1,2* 1 Hainan Provincial Institute of Tropic Agriculture Resource, Sanya, 572025 2 Hainan Provincial Key Laboratory of Crop Molecular Breeding, Sanya, 572025 3 Changli Institute of Pomology, Hebei Academic of Agriculture and Forest, Changli, 066600;The SNP Analysis of the Conserved Sequences of Ethylene Receptor Gene in Five Tropical Fruits[J];Molecular Plant Breeding;2003-01
9 Zhu Cheng (Zhejiang Unirersity, Hangzhou 310029) Liu Feiyan (Hangzhou Botanical Garden, Hangzhou 310013);To apply RAPD in classification and differentiation of cultivar of Osmanthus fragrans Lour.[J];GUIHAIA;1999-02
10 Zhang Heng-qing 1 An Li-jia 2 Zu Yuan-gang 1(1. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry Univ., Harbin 150040) (2. Chemical Institute, Dalian Science and Technology Univ., Dalian 116001);EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF PINUS KORAIENSIS[J];;1999-02
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