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Cotton Tissue Culture and Plant Regeneration

Qin Yonghua Liu Jinyuan* Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084  
Cotton (Gossypium hirsutum L.) is an important oilseed and fiber crop. Advanced biotechnology not only provides an innovation method for cotton breeding and germplasm multiply but also greatly accelerates the process of cotton breeding. The key to successful application of biotechnology in plant breeding is the establishment of an efficient regeneration system. The techniques of tissue culture and regeneration for cotton plants can be applied to cotton variety improvement, propagation and preservation of germplasm, inheritance of heterosis and genetic transformation. Therefore, in recent years, there has been increasing focus on the research of cotton tissue culture as the source of tissue explants for transforming cotton. In this paper, the recent progress on research of cotton tissue culture was reviewed including somatic cell culture, anther culture, shoot-tip culture, in vitro ovule culture and protoplast culture, and the future research works of this field were discussed.
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