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《Molecular Plant Breeding》 2006-05
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Optimization for SRAP Reaction System in Ramie (Boehmeria nivea L. Guad)

Liu Lijun Meng Zuqing Xing Xiulong Peng Dingxiang* College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070  
The factors influencing SRAP analysis, including 10×PCR Buffer, the concentration of DNA template, Mg2+, dNTP, primers, Taq polymerase, thermal cycles and annealing temperature in ramie were studied. PCR system for SRAP in ramie has been found: in 20μl reaction solution, contained 0.3μmol/L primers, 60~100ng DNA template, 2.0mmol Mg2+, 0.2mmol dNTP, 1U Taq polymerase. Amplification was carried out in two steps: in the first 5 cycles, the annealing temperature was 33℃, and during the following 30 cycles, the annealing temperature was 52℃. In all the cycles, denaturation was carried out for 1min at 94℃, annealing for 1min, and amplification for 1min at 72℃. After all the cycles, amplification was done for 1min at 72℃. It was shown that SRAP technology is an useful molecular marker system for mapping and gene tagging in ramie.
【CateGory Index】: S563.1
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【References】
Chinese Journal Full-text Database 10 Hits
1 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
2 Zhu Zhikai Fang Liangjun Zhao Qianting Deng Yanhua Su Huanjie Laboratory of Molecular Plant Breeding, College of Agronomy, Guangdong Ocean University, Zhanjiang, 524088;Optimization of TRAP-PCR Reaction System and Analysis of P-glycoprotein Gene in Rice[J];Molecular Plant Breeding;2008-01
3 Wang Xuejun Liu Guojian Chang Ruifeng Han Jicheng Guo Encai Changli Institute of Polomogy, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600;Optimization of Annealing Temperature of SRAP-PCR in 5 Temperate Fruits[J];Genomics and Applied Biology;2009-03
4 LI Xiao-hui1,WANG Cong-yan2,XU Xiao-li1,CHANG Gao-zheng1,ZHANG Si-pu1(1.Institute of Horticulture,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China;2.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China);The Optimization of SRAP-PCR Amplification Program and System for Citrullus lanatus[J];Acta Agriculturae Boreali-Sinica;2008-03
5 SHI Hong-li,HAN Ming-yu,ZHAO Cai-ping(College of Horticulture,Northwest Agricultural and Forestry University,Yangling 712100,China);Optimization of SRAP Reaction System in Peach(Prunus persica)[J];Acta Agriculturae Boreali-Sinica;2008-S2
6 SHAN Xiao-zheng1,HOU Xi-lin 1,2,LI Ying1,2,WU Hai-tao1,WANG Feng1,LI Mei1(1.National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China;2.Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,China);Optimization of SRAP System and Cluster Analysis of Non-heading Chinese Cabbage (Brassica campestris L. ssp. chinensis)[J];Jiangsu Journal of Agricultural Sciences;2009-03
7 LI Tongjian1,2,XU Lingling2,PAN Qihui3,LAI Zhanjun3,LIAO Liang2(1.College of Landscape and Art,Jiangxi Agricultural University,Nanchang Jiangxi 330045,China; 2.College of Life Sciences,Jiujiang University,Jiujiang Jiangxi 332000,China; 3.Jiangxi Institute of Bast Fiber Crops,Yichun Jiangxi 336000,China);Study Advances on the Systematics of Boehmeria[J];Jiangxi Forestry Science and Technology;2008-02
8 QIU Wen-wu1,2,SUN Wei-sheng2,DOU Mei-an2 (1.Horticultural Department,South China University of Tropical Agriculture,Danzhou 571737,China;2.Institute of Southern Subtropical Crops,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091,China);Studying Progress in SRAP——New Molecular Markers Based on PCR Reaction[J];Acta Agriculturae Jiangxi;2007-08
9 ZHANG Hui-rong1,QIAO Yu-shan2,CAO Shang-yin3,ZHANG Chuan-lai1,HU Hui-ling1,XUE Hu-bai3(1.Henan Institute of Science and Technology,Xinxiang 453003,China;2.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;3.Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China);Extraction of Genomic DNA from Mature Leaves of Some Varieties of Prunus simonii[J];Acta Agriculturae Jiangxi;2008-10
10 FENG Zhi-hong1,LI Xiao-li1,ZHAO Yu-hua2,YAN Li-ying1,PAN Yu-xia1(1 Dept of Horticulture & Garden,2 Dept of Food Engineering,Hebei Normal University of Science & Technology,Qinhuangdao Hebei,066600,China);The Optimum Annealing Temperature of SRAP-PCR on Pumpkin and the Analysis of Genetic Diversity[J];Journal of Hebei Normal University of Science & Technology;2009-03
【Citations】
Chinese Journal Full-text Database 4 Hits
1 WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China);Establishment of SRAP technique system in wheat genome[J];Journal of Agricultural University of Hebei;2005-03
2 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
3 GUO Da-long, LUO Zheng-rong*(Key Laboratory For Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, Hubei 430070 China);Optimization of SRAP -PCR in some Diospyros spp.[J];Journal of Fruit Science;2006-01
4 Li Yan, Zhang Chunqing (College of Agronomy,Shandong Agriculture University, Taian 271018);A Molecular Marker-SRAP Technique Optimization and Application Analysis[J];Chinese Agricultural Science Bulletin;2005-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
2 ZHAO Xin-liang et al (Northwest A&F University,Yangling,Shaanxi 712100);Study on Classification of Maize Inbred Lines in Henan with SRAP Marker Technology[J];Journal of Anhui Agricultural Sciences;2007-07
3 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
4 GUO Da-long et al(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Establishment and Optimization of SRAP Amplification System in Bitter Melon(Momordica charantial L.)[J];Journal of Anhui Agricultural Sciences;2008-18
5 WANG Xiao-hui1,TANG Xiao-chuang1,YANG En-xiu1,JIANG Cheng-xi1,LI Min2,LI Xiao-kun1,2(1.Ministry of Education,Engineer Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University,Changchun 130118,China;2.Pharmacy School,Wenzhou Medical College,Wenzhou,Zhejiang 325035,China);Optimization of RAPD Reaction systems for Curcuma wenyujin[J];Northern Horticulture;2008-04
6 WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China);Establishment of SRAP technique system in wheat genome[J];Journal of Agricultural University of Hebei;2005-03
7 XU Yingying,QU Shuping,CUI Chongshi(College of Horticulture,Northeast Agricultural University,Harbin 150030,China);Optimization of SRAP-PCR program and system in Chinese cabbage[J];Journal of Northeast Agricultural University;2008-08
8 LI Dongmei, ZHOU Yadong, LENG Chao, SU Yu, and LI Ming College of Agriculture, Northeast Agricultural University, Harbin 150030, China;Optimization of SRAP-PCR Reaction System in Flax[J];东北农业大学学报(英文版);2009-02
9 Fan Honghong1 Li Tingchun2 Qiu Jing1 Lin Yi1 Cai Yongping1 1 School of Life Science, Anhui Agriculture University, Heifei, 230036; 2 Anhui Provincial Tobacco Research Institute, Fengyang, 233100;Optimization of SRAP Reaction System for Plants of Dendrobium[J];Molecular Plant Breeding;2006-S2
10 Chen Daxia Li Longyun Li Quansen Qin Songyun Zhong Guoyue Chongqing Academy of Chinese Materia Medic.a,Chongqing,400065;Optimization of SRAP-PCR System for Curculigo L.Plant[J];Molecular Plant Breeding;2007-S1
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 He Qiwei,Zhao Shuangyi,Shi Huilian,An Zhenqin,Lang Fengqing & Wang Shufen (Vegetable Institute,Shandong Academy of Agricultural Sciences,Jinan 250100);Preliminary Study on Skin Color Inheritance of Chinese Radish[J];SHANDONG AGRICULTURAL SCIENCES;1997-02
2 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
3 XU Li et al(Shenyang Agricultural University,Shenyang,Liaoling 110161);Optimal of SSR-PCR Polyacrylamide Gel Electrophoresis Condition in Sorghum[J];Journal of Anhui Agricultural Sciences;2007-02
4 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
5 QIN Wei-hua,ZHOU Shou-biao,WANG Heng-ying,WANG Hui(College of Life Science,Anhui Normal University,Wuhu 241000,China);ADVANCES IN LYCORIS HERB[J];Journal of Anhui Normal University(Natural Science);2003-04
6 WANG Ying~(1,2),ZHAO Hua-bin~1,HAO Jia-sheng~1(1. College of Life Sciences, Anhui Normal University, Wuhu 241000, China;2.Wuhu Hygienic School, Wuhu 241000, China);THEORY METHOD AND PERSPECTIVE OF MOLECULAR PHYLOGENETICS[J];Journal of Anhui Normal University(Natural Science);2005-01
7 Ren Xiufang; Zhou Shoubiao; Chu Xiaohui; Xu Lin(Department of Biology);Studies on the Pollen Morphology of 6 Species of Lycoris Herb. in China[J];Journal of Anhui Normal University(Natural Science);1994-01
8 Ke Lixia Sun Yegen 1 Zheng Yan Zhang Dingcheng (Department of Biology,Anhui Normal University,241000,Wuhu, Anhui, PRC) ( 1Departmaent of Geography,Anhui Normal University, 241000, Wuhu, Anhui, PRC);KARYOTYPE ANALYSIS OF THREE SPECIES OE LYCORIS HERB[J];Journal of Anhui Normal University(Natural Science);1998-04
9 NIE liu wang, ZHANG Ding chen, ZHANG Hai jun, ZHANG Ke yun (Department of Biology, Anhui Normal University ,241000,Wuhu, Anhui, China);A STUDY ON ISOZYMES OF 5 SPECIES IN POLYYONATUM MILL[J];JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE);1999-01
10 WANG Lan 1* , HAN Yan-chuang 1* , PENG Yu-lu 1 , TENG Cai-zhu 1 , ZHOU Ming-quan 2** , HU Zhong-li 1, 2** , SONG Yun-chun 1 ,2 (1 Key Lab of the Ministry of Education for Plant Developmental Biology, Wuhan University, Wuhan 430072;2 Lotus center , Wuhan University, Wuhan 430072, China);Application of ISSR Technique to Genetic Research of Lotus Root[J];Amino Acids & Biotic Resources;2004-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI Dongmei, ZHOU Yadong, LENG Chao, SU Yu, and LI Ming College of Agriculture, Northeast Agricultural University, Harbin 150030, China;Optimization of SRAP-PCR Reaction System in Flax[J];东北农业大学学报(英文版);2009-02
2 Wang Xuejun Liu Guojian Chang Ruifeng Han Jicheng Guo Encai Changli Institute of Polomogy, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600;Optimization of Annealing Temperature of SRAP-PCR in 5 Temperate Fruits[J];Genomics and Applied Biology;2009-03
3 ZHAO Sheng-jie,LIU Wen-ge,YAN Zhi-hong,HE Nan(Zhengzhou Fruit Research Institute,CAAS,Zhengzhou,Henan 450009 China);Studies on fingerprinting of seedless triploid watermelon cultivars and hybrid purity identification by SRAP markers[J];Journal of Fruit Science;2009-05
4 YAO Jian-hua1,FU Hong-tuo1,2,GONG Yong-sheng2,WU Yan2,HE Xin-long2,LI Hui-ping1,QIAO Hui1(1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;2.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China);Orthogonal Design and Optimization of SRAP Amplification System for Macrobrachium nipponense[J];Acta Agriculturae Boreali-Sinica;2008-S2
5 XU Jian-hua1,YANG Hong-qi1,YANG Cheng2,ZHOU Ji-heng1,DUAN Feng-yun2,CHEN Xin-bo1,ZHAN You-guo1,ZHAO Shi-hao1(1.Key Laboratory of Tobacco Science and Healthy,HNAU,Changsha 410128,China;2.Technology Center Tobacco Dali Corp,Dali,Yunnan 671000,China);DNA extraction and optimization of SRAP reaction system in tobacco[J];Journal of Hunan Agricultural University(Natural Sciences);2009-03
6 Yu Chaoguang Yin Yunlong Xu Jianhua(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences Nanjing 210014);Identification of Taxodium Hybrids by SRAP Analysis[J];Scientia Silvae Sinicae;2009-02
7 GU Feng-ping,ZHOU Chun-e,LU Shu-xia,YAO Huan-ling,WANG Fang,DUAN Hong-ying,ZHOU Yanq-qing(College of Life Sciences,Henan Normal University,Xinxiang 453007,China);Optimization of Amplification and Construction of DNA Fingerprinting for Rehmannia Glutinosa Libosh. f. Hueichingensis (Chao et Schih) Hsiao Based on SRAP[J];Journal of Henan Normal University(Natural Science);2009-03
8 GUO Rui1,LI Xiao-yan1,WANG Li-rong2,WANG Guo-shan3(1.College of Agronomy,InnerMongolia Agricultural Institute,Huhhot 010019,China;2.Chinese Academy of Agricultural Sciences Zhengzhou Fruit Tree Institute,Zhengzhou 450093,China;3.The Foodstuff Production Base of Military District in Inner Mongolia,Huhhot 010010,China);Peach SRAP System Optimization and SSR Peach Varieties in the Comparative Identification[J];Acta Agriculturae Boreali-Sinica;2009-04
9 FENG Zhi-hong1,LI Xiao-li1,ZHAO Yu-hua2,YAN Li-ying1,PAN Yu-xia1(1 Dept of Horticulture & Garden,2 Dept of Food Engineering,Hebei Normal University of Science & Technology,Qinhuangdao Hebei,066600,China);The Optimum Annealing Temperature of SRAP-PCR on Pumpkin and the Analysis of Genetic Diversity[J];Journal of Hebei Normal University of Science & Technology;2009-03
10 ZOU Ming-hong1,GUO Ling-fei2,ZENG Hui1,DU Li-qing1,LU Chao-zhong1,LUO Lian-fang1,ZHANG Han-zhou1 (1.South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091,Guangdong,China;2.Guangxi Subtropical Crops Research Institute,Nanning 530001,Guangxi,China);Genome DNA Extraction and SRAP Reaction System Optimization of Garcinia mangostana[J];Nonwood Forest Research;2009-03
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
2 ZHANG Jun, WU Yao ting, GUO Wang zhen, ZHANG Tian zhen (Agronomy Department, Nanjing Agricultural University; Key Laboratory of Crop Germplasm and Breeding, Ministry of Agriculture, Nanjing 210095, China);Fast Screening of Microsatellite Markers in Cotton with PAGE/silver Staining[J];ACTA GOSSYPII SINICA;2000-05
3 LIN Zhong-Xu,ZHANG Xian-Long~①,NIE Yi-Chun (National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China);Evaluation of Application of a New Molecular Marker SRAP on Analysis of F_2 Segregation Population and Genetic Diversity in Cotton[J];Acta Genetica Sinica;2004-06
4 WANG Feng-ge,ZHAO Jiu-ran,GUO Jing-lun,LIU Long-zhou(Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing100089,China);Series of Research on Establishing DNA Fingerprinting Pool of Chinese New Maize Cultivars Ⅰ.The Establishment of a Standard SSR System Fitting for Maize Cultivars' Identification[J];Journal of Maize Sciences;2003-01
5 Luo Zhengrong,Li Fafang,and Cai Lihong (Laboratory of Pomology,Department of Horticulture,Huazhong Agricultural University,Wuhan 430070);Molecular Systematics of China Native Nonastringent Persimmon Based on Random Amplified Polymorphic DNA[J];ACTA HORTICULTURAE SINICA;1999-05
6 PAN Qing-hua, HU Tie-zhu, WANG Ling, CAI Jiang-qiao (Laboratory of Plant Resistance and Genetics, College of Natural Resource Environment, South China Agricultural University, Guangzhou 510642);Molecular Genetic Studies on the Rice Blast Fungus Population DNA fingerprinting and pathotype analyses of the isolates selected from the region-specific lineages of Guangdong Province, China[J];Scientia Agricultura Sinica;2004-01
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