Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2006-S1
Add to Favorite Get Latest Update

Cloning Methods of Plant Gene Promoters and Their Applications

Yin Hui Li Dan Zhang Yi Li Qiuli* College of Life Sciences, Liaoning Normal University, Dalian, 116029  
The expression and regulation of plant gene has been the hotspot study in molecular biology. Promoter is an important cis-acting element. Cloning promoter is important to study gene regulation, vectors construction and target proteins expression. From the frequently used way that applying promoter trapping for choosing promoter to the using of PCR, there were lots of ways for promoter cloning. Afterwards, a series of techniques based on PCR for promoter cloning such as A-PCR, I-PCR, LA-PCR, TAIL-PCR were developed in succession. They offered more reliable and reasonable ways of cloning the promoter. Some ways of cloning plant gene promoters were introduced, meanwhile the limitation of these ways was introduced and their developing prospect was also discussed in this paper.
【CateGory Index】: Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 WU Tian1,2,XIE Cong-hua2(1College of Horticulture and Gardening,Southwest Forestry University,Kunming 650224;2 College of Horticulture and Forestry,Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology,Ministry of Education/National Center for Vegetable Improvement(Central China)/Potato Engineering & Technology Research Center of Hubei Province,Wuhan 430070);Cloning and Activity Analysis of the Promoter of Potato Protein Kinase Gene StPK1[J];Scientia Agricultura Sinica;2011-05
2 GOU Chun-Bao,WANG Yong,YU Chuan,CHEN Fang,WEI Wei Key Laboratory of Bio-resource and Eco-environment,Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China;Isolation of MIPS Gene Promoter from Jatropha curcas L.and Activity Analysis of Transient Expression in Tobacco Protoplast[J];Plant Physiology Communications;2010-07
China Proceedings of conference Full-text Database 1 Hits
1 Ma Guang,Guo Jiping,Liu Zhihua Department of Life Sciences Hengshui College Hengshui,China,053000 Zhou Bo,Wang Yu,Li Yuhua College of Life Sciences Northeast Forestry University Harbin,China,150040;Cloning and analysis on promoter region of F3H gene in Brassica rapa L. ssp. rapifera by bioinformation[A];[C];2010
Chinese Journal Full-text Database 6 Hits
1 WANG Li-jun~1, FAN San-hong~2,GUO Ai-guang~(2*) (1 College of Life Science, Northwest Sci-Tech University of Agriculture and Foresf Shaanxi Province, Yangling, Shaanxi 712100,China);Cloning and function analysis of ats1A gene promoter from Arabidopsis thaliana[J];Acta Botanica Boreali-occidentalia Sinica;2004-10
2 Han Zhiyong Wang Xinqi Shen Gezhi (Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106);CLONING OF FOREIGN GENE'S FLANKING SEQUENCES IN TRANSGENIC RICE BY INVERSE PCR[J];Acta Agriculturae Shanghai;2001-02
3 LIU Zhao_Hua 1,2 , GUO Hong_Nian 1 , ZHENG Guang_Yu 2 and TIAN Ying_Chuan 1* 1 Institution of Microbiology Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080, China 2 Beijing Normal University, Beijing 100875, China;Cloning of ACA Gene Promoter and Preliminary Study of its Function[J];Chinese Journal of Biotechnology;2005-01
4 LI Qiu-Li, ZHANG Yi, YIN Hui and LI DanCollege of Life Science, Liaoning Normal University, Dalian 116029, China;Isolation of BADH Gene Promoter from Suaeda liaotungensis and its Sequence Analysis[J];Chinese Journal of Biotechnology;2006-01
5 WANG Xin\|guo, XIAO Cheng\|zu * (Institute of Biotechnology,Academy of Military Medical Sciences,Beijing\ 100071,China) ZHANG Guo\|hua,FANG Rong\|xiang (Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China);Isolation of a Novel Carrot Invertase Ⅱ Promoter by Adaptor-PCR[J];Chinese Journal of Biochemistry and Molecular Biology;2001-01
6 CAI-YIN Qing-Ge-Le, LI Ming-Chun, CAI Yi, TAO Ran, XING Lai-Jun *(Department of Microbiology, College of Life Science, Nankai University, Tianjin 300071, China);Isolation and Sequencing Analysis of the Seed-specific Promoter from Soybean[J];Acta Agronomica Sinica;2005-01
Chinese Journal Full-text Database 10 Hits
1 WANG Kui-di et al(Institute of Environment and Plant Protection,CATAS,Danzhou,Hainan 571737);A Rapid and Efficient Method of Isolating the Flanking Sequence of T-DNA Inserted Site-DW-ACP-PCR Technique[J];Journal of Anhui Agricultural Sciences;2007-28
2 ZHAO Hao-qin et al(Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212003 );Cloning and Sequence Analysis of Biological Clock Gene Bmtim2 in Bombyx mori L.[J];Journal of Anhui Agricultural Sciences;2008-17
3 LIU Chun-xiang (Weifang University,Weifang,Shandong 261061);PCR-Walking Techniques Based on Random Primer[J];Journal of Anhui Agricultural Sciences;2010-06
4 YANG Kun et al (College of Chemistry and Life Science,Zhejiang Normal University,Jinhua,Zhejiang 321004);Study on the Isolation of the Flanking Sequences Adjacent to Transgenic T-DNA in Brassica napus Genome by an Improved Inverse PCR Method[J];Journal of Anhui Agricultural Sciences;2010-10
5 WANG Jing (Inner Mongolia Key Laboratory of Herbage and Endemic Crop Biotechnology,Biotechnology Center,College of Life Sciences,Inner Mongolia University,Hohhot 010021);Research Progress of IPCR on Cioneing Flanking Sequence[J];Northern Horticulture;2010-03
6 HAO Diqiu,ZHAO Lin,CHEN Limiao,LI Wenbin(Key Laboratory of Soybean Biology,Ministry of Education,Soy-bean Research Institute,College of Agriculture,Northeast Agricultural University,Harbin 150030,China);Comparison and application of promoter clone methods[J];Journal of Northeast Agricultural University;2010-03
7 YAN Jing-wan~(1,2), LIN Lin~1, WANG Feng~2(1. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China; 2. Key Laboratory of Agriculture Genetic Engineering, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China);Amplified methods for acquiring gene flanking sequence's information[J];Fujian Journal of Agricultural Sciences;2005-S1
8 Zhang Junlian1,2 Wang Di1,2 Zhang Jinwen1,2 Chen Zhenghua3 1 Agronomic College of Gansu Agricultural University,Lanzhou,730070;2 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement,Lanzhou,730070;3 Postdoctor Workstation of Yasheng Industrial Ltd.,Beijing,100101;Modification of pBⅠ121 Vector and Expression Vector Construction Na~+/H~+ Antiporter of Arabidopsis thaliana[J];Molecular Plant Breeding;2006-06
9 TIAN Hua-ying1,PANG Cai-hong2,XIA Yang2*,BAO Ying1,XIAN Yang2, LI Shuang-yun2,LI Li2,LIU Cui-lan2,MAO Xiu-hong2,YAN Li-ping2(1.College of Life Science,Qufu Normal University,Qufu 273165,China;2.Shandong Provincial Key Laboratory of Forest Tree Genetic Improvement/ Shandong Academy of Forestry,Jinan 250014,China);Construction of Plant Expression Vector of Amaranthus caudatus Agglutinin Gene(ACA)[J];Shandong Agricultural Sciences;2011-06
10 Lai Pengcheng(College of Life Sciences,Fujian Normal University,Fuzhou 35000,China);The Research on the Specific Promoters of Higher Plants[J];Anhui Agricultural Science Bulletin;2011-03
Chinese Journal Full-text Database 10 Hits
1 LIU\ Fuquan (College of Life Sciences,Peking University,Beijing,100871) ZHANG\ Yanwen (Department of Biology,Nankai University,Tianjin,30071) XU\ Zhihong (Chinese Academy of Sciences,Shanghai Institute of Plant Physiology,Shanghai,200032);Cloning and Primarily Detecting the Function of the Promoter Region of NFL Gene in Tobacco[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;2000-02
2 Zeng Qingyin; Lu Hai; Fu Xueqi; Wang Shasheng; Jiang Xiangning.College of Life Science, Jilin University, Changchun, 130023, P.R.China.;Functions of Xylem-Specific-Expression Promoter of Glycine-Rich-Protein (GRP 1.8) Gene from Ginkgo biloba in transgenic tobacco plants.[J];Journal of Beijing Forestry University;2002-01
3 QI Chun-hui;HAN Lie-bao;LIANG Xiao-hong;ZENG Hui-ming;LIU Jun.Key Laboratory for Silviculture and Conservation,Ministry of Education,Institute of Turfgrass Science,Beijing Forestry University,100083,P.R.China.;Transgenic Zoysia japonica plants obtained by biolistic bombardment transformation.[J];Journal of Beijing Forestry University;2006-03
4 YANG Qing-chuan1, SUN Yan2, KANG Jun-mei1(1. Institute of Animal Science, CAAS, Beijing 100094, China; 2. Institute of Grassland Science, China Agriculture University, Beijing 100094, China);Research on the Advancement of Salt Tolerant Gene in Alfalfa[J];Acta Agrestia Sinica;2005-03
5 KUANG Shou-Long1,HU Ting-zhang2(1.Yangtze Normal University,Chongqing 408003,China;2.Department of Biology,Chongqing Three Gorges University;Chongqing 404000,China);The Cloning and Research Methods of Promoters[J];Journal of Chongqing Institute of Technology(Natural Science Edition);2007-01
6 WANG Dan,WANG Pi-wu,FU Yong-ping,LI Zhi(College of Agriculture,Jilin Agricultural University,Changchun 130118,Jilin,China);Cloning and Identification of the Root Specific Promoter from Soybean[J];Soybean Science;2009-02
7 WANG Ying,MAI Wei-jun,LIANG Cheng-ye,ZHANG Ming-yong~*(South China Institute of Botany,Chinese Academy of Sciences,Guangzhou 510650,China);Advances on studies of plant promoters[J];Acta Botanica Boreali-occidentalia Sinica;2003-11
8 WANG Li-jun~1, FAN San-hong~2,GUO Ai-guang~(2*) (1 College of Life Science, Northwest Sci-Tech University of Agriculture and Foresf Shaanxi Province, Yangling, Shaanxi 712100,China);Cloning and function analysis of ats1A gene promoter from Arabidopsis thaliana[J];Acta Botanica Boreali-occidentalia Sinica;2004-10
9 ZHAO Chang-ling,GUO Wei-ming,YANG Qing,CHEN Jun-yu(College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; College of Agricultural Sciences and Biotechnology,Yunnan Agricultural University,Kunming 210095,China;College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China);Cloning of Full-length F3'H of Nanjinghongxu (Prunus mume)by gDNA-based TAIL-PCR[J];Acta Botanica Boreali-occidentalia Sinica;2005-12
10 FAN Li-na,DENG Hai-hua,QI Yong-wen(Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery,Guangzhou Sugarcane Industry Research Institute,Guangzhou 510316,China);Research Advances in CO Genes of Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2008-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiao guo 2,LIU Yu le 1,YANG Qing ping 2,TIAN bo 1 (1 Institute of Microbology,Chinese Academy of Science,Beijing 100080,China;2 College of Life Science,Wuhan University,Wuhan 430072,China);Sequence and functional analyses of the tapetum-specific promoter Osg6B from rice (Oryza sativa L.)[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;2000-05
2 HOU Wen sheng 1,GUO San dui 2,LU Ming 1(1 Yangling Branch of China Wheat Improvement Center,College of Agronomy,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Biotechnology Research Institute,Chinese Academ;Construction of high-level expression vectors containing sgna gene for use in wheat Transformation[J];Acta Botanica Boreali-occidentalia Sinica;2002-05
3 Shen Gezhi; Zhang Jianjun; Yin Liqin; Wang Xinqi; Chen Quanqin; Fan Kunhua (Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences, Shanghai 201106);Agrobacterium-MEDIATED Ds TRANSPOSON TRANSFORMATION IN RICE (Oryza sativa L.)[J];ACTA AGRICUL TURAE SHANGHAI;1998-04
4 Liang Hua Ma Chonglie Zhao Qian Ao Guangming (National Labs for Agrobiotech. , Beijing Agriculture Univercity, Beijing 100094);Amplification of Maize Zein Gene Promoter by PCR and It Drive GUS Gene Expression in Transgenic Tobacco Seeds[J];Chinese Journal of Biotechnology;1996-03
5 YING Ge\ WU Wei\ HE Chao Zu * (Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China);Cloning of Xanthomonas campestris pv.campestris Pathogenicity related Gene Sequences by TAIL-PCR[J];Chinese Journal of Biotechnology;2002-02
6 WANG Huai; CHEN Zheng-Hua(Institute of Genetics, Chinese Academy of Science, Beijing 100101);Nuclear matrix attachment regions and transgene expression in plants[J];CHINESE BULLETIN OF LIFE SCIENCES;1999-02
7 Chen Feng\ Li Jie\ Zhang Gui\|you\ Liu Qiang (Department of Biological Sciences and Biotechnology,Tsinghua University, Beijing\ 100084);The Mechanism and Application of Yeast One-hybrid System[J];Progress In Biotechnology;2001-04
8 Xu Junwang Li Xugang Zhu Zhen Institute of Genetics,Chinese Academy of Sciences,Beijing 100101;Manipunation of Starch Quality via Genetic Engineering[J];BIOTECHNOLOGY INFORMATION;2000-01
9 Shan Song Zhang Zhonglin Chen Xi Liu Chunying Shen Guifang(Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081);Cloning and Modification of 16S Promoter of Tobacco Chloroplast Genome and Production of Transgenic Plants[J];Acta Agronomica Sinica;1999-05
10 Li-Yue You,Xin Liu Li-Yue You,Xin Liu,Department of Molecular Genetics,Third Military Medical University of Chinese PLA,Chongqing 400038,China;Biological effect of up-regulated esp1 gene expression in lung adenocarcinoma cell[J];Chinese Journal of Clinical Rehabilitation;2004-35
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved