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《Molecular Plant Breeding》 2006-S1
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Cloning Methods of Plant Gene Promoters and Their Applications

Yin Hui Li Dan Zhang Yi Li Qiuli* College of Life Sciences, Liaoning Normal University, Dalian, 116029  
The expression and regulation of plant gene has been the hotspot study in molecular biology. Promoter is an important cis-acting element. Cloning promoter is important to study gene regulation, vectors construction and target proteins expression. From the frequently used way that applying promoter trapping for choosing promoter to the using of PCR, there were lots of ways for promoter cloning. Afterwards, a series of techniques based on PCR for promoter cloning such as A-PCR, I-PCR, LA-PCR, TAIL-PCR were developed in succession. They offered more reliable and reasonable ways of cloning the promoter. Some ways of cloning plant gene promoters were introduced, meanwhile the limitation of these ways was introduced and their developing prospect was also discussed in this paper.
【CateGory Index】: Q943.2
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