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《Molecular Plant Breeding》 2007-05
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Identification of Pummelo (Citrus grandis (L.) Osbeck) and Hybrid Citrus Cultivars using SSR Molecular Markers

Lei Tiangang1,2,3 He Yongrui1,2,3 Wu Xin2 Liu Xiaofeng1,2,3 Xu Lanzhen1,2,3 Peng Aihong1,2,3 Chen Shanchun1,2,3 1 Citrus Research Institute,CAAS,Chongqing,400712;2 Citrus Research Institute,Southwest University,Chongqing,400712;3 National Citrus Cultivar Improvement Center of China,Chongqing,400712  
Simple sequence repeat(SSR)was used to construct the fingerprinting of mainly cultivars of pummelo(Citrus grandis(L.)Osbeck)and hybrid citrus in China.A total of ten primer pairs with stable and repeatable polymorphism that can identify closely related citrus cultivars were screened out from 105 SSR primer pairs.In this paper,five pummelo and seven hybrid citrus cultivars were analyzed by using five of these SSR markers.As a result,a total of 35 bands were detected,27 of which were polymorphic.The polymorphic rate of each primer pair was beyond 60%.The bands range in size was from 100 bp to 500 bp.The fingerprinting of five pummelo and seven hybrid citrus cultivars was constructed by using two particularly high efficiency primer groups(SSR17、SSR15、SSR16、SSR12 and SSR17、SSR18、SSR16)respectively.Furthermore,the mode fingerprinting datebase of citrus cultivars was established by fingerprint analysis system-Gel 2.0.
【Fund】: 科技部科研院所社会公益研究专项(2004DIB45147);; 重庆市科技攻关重点项目(CSTC 2006AB1009)
【CateGory Index】: S666
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