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Expression Pattern of DBP12 Promoter of DlBADH1 Gene from Dendran the ma lavandulifolium

CaoHuawen~1 LiuZhenlin~(1,3) XiaXinli~2 YinWeilun~2 DaiSilan~(1*) 1 College of Landseape Architecture,Beijing Forestry University,Beijing,100083; 2 College of Biology Science and Technology,Beijing Forestry University,Beijing,100083; 3 Department of Horticulture and Landscape Architecture,Hebei Normal University of Science and Technology,Changli,066600  
The construct pCHW-2 consisting of DBP12 promoter(Genbank Accession No:DQ497621)of Dl- BADH1(Genbank Accession No:DQ011151)gene from Dendranthema lavandulifolium L.genome was trans- ferred into tobacco plants via A grobacterium tumefaciens EHA105.The regenerated plants were analyzed by GUS histo-chemical staining.The results showed that the expression of GUS gene under the control of DBPl2 promoter was no tissue-specific.Under the treatments of NaCl,ABA and SA stress,the expression efficiency of the promot- er in transgenic plants were analyzed by GUS activity assay.The level of GUS activity in the leaves of transgenic tobacco plants increased steadily after 24 h exposure tO 100μmol/L ABA and 48 h exporsure to 400 mmol/L Na- Cl,which could imply that DBPl2 promoter of DlBA DH1 gene should be an inducible promoter.The results might provide a reference for further studies on expression pattern of BADH gene in Dendranthema lavandulifolium.
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