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《Molecular Plant Breeding》 2007-06
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Extraction and Quality Analysis of Total RNA from Coconut Pulp Tissue

Li Dongdong~* Fan Yongmei Department of Biotechnology,College of Science and Technology,Hainan University,Haikou,570228  
In this research,we employed and modified the common CTAB extraction method,then used the mod- ified procedures to isolate total RNA from coconut pulp tissue(Cocos nucifera L.).The results showed that the modified protocols could be capable of efficiently removing different kinds of substances such as polysaccharides etc.,and we would obtained high-quality total RNA from two types of coconut pulp,maturated and unmaturated, which both of them were validated by the bright bands of 28S and 18S rRNA on 1% agarose gel electrophoresis,as well as the values between 1.6 and 1.9 of OD_(260)/OD_(280).The total RNA concentrations of the tested samples were 0.070μg/μL and 0.053μg/μL,respectively.It would be suggestions that that the modified CTAB protocols could be used for isolating high-quality and completely RNA.
【Fund】: 本研究得到了国家自然科学基金(30560092);; 海南省自然科学基金(80406);; 海南省教育厅高校科研资助项目(Hjkj200510)的资助
【CateGory Index】: S667.4
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