Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2007-S1
Add to Favorite Get Latest Update

Optimization of SRAP-PCR System for Curculigo L.Plant

Chen Daxia Li Longyun Li Quansen Qin Songyun Zhong Guoyue Chongqing Academy of Chinese Materia Medic.a,Chongqing,400065  
In this research SRAP reaction conditions were optimized by single or dual factor experiment that in- volved in the main reaction components,i.e.the concentration of Mg~(2+),dNTP,primer,and template DNA,as well as Taq DNA polymerase dosage.The products of SRAP were identified both by agarose gel and non-denaturing polyacrylamide gel electrophosresis.It was obvious that the optimized reaction system would be suitable for PCR ananlysis of Curculigo plant,which might be the 25μL amplification reaction system containing 1 xPCR buffer, 2.0 mmol/L Mg~(2+),250μmol/L dNTP,0.2μmol/L primer,40 ng template DNA,and 1 U Taq DNA polymerase. And also it is worthy mentioned that the non-denaturing PAGE approach would be more productive and informa- tive for SRAP-PCR,which could obtain the high resolution banding patterns.
【Fund】: 重庆市科委攻关项目(6455)
【CateGory Index】: Q943
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 CHEN Daxia1,PENG Rui1,LI Longyun1,SUN Nianxi1,ZHONG Guoyue 1,CAI Yingfan2(1.Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China;2.Chongqing University of Posts and Telecommunications,Chongqing 400065,China);Study on genetic diversity of Codonopsis tangshen by SRAP and ISSR markers[J];China Journal of Chinese Materia Medica;2009-03
Chinese Journal Full-text Database 6 Hits
1 WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China);Establishment of SRAP technique system in wheat genome[J];Journal of Agricultural University of Hebei;2005-03
2 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
3 LIN Zhongxu, ZHANG Xianlong, NIE Yichun, HE Daohua & WU Maoqing National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China;Construction of a genetic linkage map for cotton based on SRAP[J];科学通报(英文版);2003-19
4 Liang Jingxia1 Qi Jianmin1 Wu Weiren1 Zhou Dongxin2 Chen Shunhui3 Wang Tao1 Tao Aifen1 1. Fujian Agriculture and Forestry University,Fuzhou 350002 2. Longyan Branch of Institute of Fujian Tobacco Agricultural Science, Longyan 364000 3. Institute of Fujian Tobacco Agricultural Science, Fuzhou 350003;DNA extraction and the establishment of SRAP reaction system in tobacco[J];Acta Tabacaria Sinica;2005-04
5 Li Yan, Zhang Chunqing (College of Agronomy,Shandong Agriculture University, Taian 271018);A Molecular Marker-SRAP Technique Optimization and Application Analysis[J];Chinese Agricultural Science Bulletin;2005-05
6 WEN Yan-cheng1,2, WANG Han-zhong1, SHEN Jin-xiong1, LIU Gui-hua1, ZHANG Shu-fen2 (1 Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062; 2Cotton and Oil Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002);Analysis of Genetic Diversity and Genetic Basis of Chinese Rapeseed Cultivars (Brassica napus L.) by Sequence-Related Amplified Polymorphism Markers[J];Scientia Agricultura Sinica;2006-02
Chinese Journal Full-text Database 10 Hits
1 Li Yan et al.(Hunan agricultural University,HunanChangsha 410128);Research Advances on Germplasm Resourse in Tobacco[J];Anhui Agricultural Science Bulletin;2008-09
2 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
3 ZHAO Xin-liang et al (Northwest A&F University,Yangling,Shaanxi 712100);Study on Classification of Maize Inbred Lines in Henan with SRAP Marker Technology[J];Journal of Anhui Agricultural Sciences;2007-07
4 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
5 DING Jin-ping et al (College of Agronomy,Guangxi University,Nanning,Guangxi 530003);Establishment and Optimization of SRAP Reaction Conditions for Arachis hypogaea L.[J];Journal of Anhui Agricultural Sciences;2008-10
6 GUO Da-long et al(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Establishment and Optimization of SRAP Amplification System in Bitter Melon(Momordica charantial L.)[J];Journal of Anhui Agricultural Sciences;2008-18
7 DONG Yun et al(Crop Institute of Gansu Academy of Agriculture Science,Lanzhou,Gansu 730070);Application Progress of Molecular Marker Technologies in Genetic Diversity Study of Brassica napus L.[J];Journal of Anhui Agricultural Sciences;2009-18
8 TIAN Ming-li,WU Xiao-bing(College of Life Sciences,Anhui Normal University,Wuhu 241000,China);Optimization of Experiment Conditions and Primer Screening With SRAP Markers for Chinese Alligators[J];Journal of Anhui Normal University(Natural Science);2008-02
9 DING Wei-Dong,CAO Li-Ping ,CAO Zhe-Ming Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu Province,China;The SRAP and SCAR molecular markers for detecting germ degeneration in Ctenopharyngodon idellus[J];Acta Zoologica Sinica;2008-03
10 LI Hai-xia;ZHANG Zhi-yi;ZHANG Qian;AN Xin-min;LI Yan;LIU Ting-ting. National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing Forestry University,100083,P.R.China.;Transformation of tobaccos with a TIR-NBS-LRR gene isolated from Populus tomentosa Carr[J];Journal of Beijing Forestry University;2009-01
China Proceedings of conference Full-text Database 10 Hits
1 REN Yu YANG Guang-sui YIN Jun-mei (Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou 571737);Analysis of Genetic Diversity in Dendrobium Germplasm by SRAP Makers[A];[C];2007
2 ;SRAP Molecular Marker Related to Green Peel Gene in Cucumber[A];[C];2008
3 ;Cultivar Identification and Fingerprinting of Eggplant (Solanum melongena L.) with Molecular Markers[A];[C];2008
4 FU Fuyou, LI Jiana Chongqing Rapeseed Technology Research Center; Chongqing Key Laboratory of Crop Quality Improvement; Key Lab of Biotechnology & Crop Quality Improvement of Ministry of Agriculture; College of Agronomy and Biotechnolodgy, Southwest University, 216 Tiansheng Road, Beibei, Chongqing, 400716, P. R. China.;A molecular genetic linkage map in recombinant inbred lines of Brassica napus L. using SSR and SRAP markers[A];[C];2007
5 WEN Yancheng1, 2, WANG Hanzhong1, ①, SHEN Jinxiong1, LIU Guihua1 1. Institute of Oil Crops, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China 2. Institute of Cotton and Oil Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China;Genetic evolution of Brassica crops revealed with srap markers[A];[C];2007
6 Jianming GAO,Zhongyou PEI,Qiuling CHEN,Jinzhao WEI,Zifang Li Feng LUO,Bo DING,Shoujun SUN* (Department of Agronomy,Tianjin Agricultural University,Tianjin 300384,P.R.China;;AFLP and SRAP fingerprinting of a collection of sweet sorghum germplasm[A];[C];2008
7 XU Xiuhong, WANG Yuanying, WANG Honggang, WANG Shaomei, ZHANG Chengsheng (Qingzhou Tobacco Research Institute of CNTC, Qingdao 266101, China; Agronomy College of Shandong Agricultural University, Tai'an 271018, China);Identification and marker-assisted selection of molecular marker linked to the gene conferring resistance to tobacco wildfire[A];[C];2005
8 Pian Rui-qi (Laboratory for Biotechnology in Tropical and Subtropical Forest Science; College of forestry; South China Agricultural University Guangzhou 510642; Biological Institute, Beijing Forestry University Beijing 100083) Ni Hui-qun Chen Xiao-yang (Biological Institute, Beijing Forestry University Beijing 100083);Optimization of SRAP System and Genetic Relationship Analysis of Lespedeza[A];[C];2009
9 CHEN Gang FENG Jian WANG Qian-chun PAN Wen-li FAN Jun-gang (Liaoning Research Academy of Forestry Sciences Shenyang 110032);SRAP Marker and Its Prospect of the Application in Forest Tree Genetic Breeding[A];[C];2009
10 LIZHONG FU~(1,2),HONGYU ZHANG~3,XUEQIAN WU~(1,2,*),HAIBO LI~1,HAILONG WEI~(1,2), QINGQI WU~(1,2) & LIAN WANG~3 1 Zhejiang Academy of Forestry Sciences,Hangzhou,China;2 Lishui Edible Fungi Research & Development Center,China;3 College of Life Sciences,Hebei Normal University,Shijiazhuang,China;Use of Sequence-Related Amplified Polymorphism Maker to Analysis Genetic Diversity of Lentinula edodes[A];[C];2009
Chinese Journal Full-text Database 10 Hits
1 NIE Song liu,XU Xian xiang,XIA Lun zhu (Hospital of Lu′an City,Anhui Lu′an 237000,China);EFFECT OF TOTAL SAPONINS OF CODONOPSIS ON BLOOD LIPID AND NITRIC OXIDE LEVEL IN EXPERIMENTAL HYPERLIPEMIA RATS[J];Journal of Anhui Traditional Chinese Medical College;2002-04
2 LIU Wen-sheng,XUE Lin-li,WEI Ping (College of Amimal Techndogy and Science, Shanxi Agricultural University, Shanxi Taiyuan 030801,China);Extraction and Determination of the Polysaccharide in Radix Codonpis Pilosulae[J];Journal of Anhui Traditional Chinese Medical College;2005-01
3 MA Huiling,HAN Xuewen,DONG Juane ZHANG Boyong,ZHANG Anlingof The Exploitation and Utilization of Economic Plant Resources,Shaanxi Province,Yangling Shaanxi 712100,China);Study on the relation between the dynamic accumulation of secondary metabolites and the phenological period of Eucommia ulmoides Oliv.[J];Acta Botanica Boreali-occidentalia Sinica;2003-09
4 DONG Juan-e,LIANG Zong-suo (Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China);Analysis on the factor influencing secondary metabolite accumulation in plants[J];Acta Botanica Boreali-occidentalia Sinica;2004-10
5 CHAO Zhi1,WANG E-zhou2,ZHOU Xiu-jia3 (1Department of Traditional Chinese Medicine, First Military Medical University, Guangzhou 510515, China; 2College of Pharmacy, Shanghai Medical University, Shanghai 200032, China; 3College of Traditional Chinese Mat;Relationship between alkaloid contents and growth environment of Yimu Cao(Herba Leonuri)[J];Journal of First Military Medical University;2000-06
6 Si Youlin, Jiao Dong'gui,Sun Yuandong (Bengbu Medical College,Anhui 233003);Determination the Content of Four Trace Elements of Dangshen[J];Primary Journal of Chinese Materia Medica;2001-04
7 CHEN Da-Xia1 LI Long-Yun1 LU Cheng2 ZHONG Guo-Yue1 QU Xian-You1 PENG Rui1(1.Chongqing Academy of Chinese Materia Medica,Chongqing 400065)(2.The Key Sericultural Laboratory of Southwest Agricultural University,Chongqing 400716);Study on Optimization of ISSR Reaction Conditions for Coptis Chinensis Franch[J];Bulletin of Botanical Research;2007-01
8 Zhang Wancheng(Molecular Biology Institute,Sichuan Normal University,Chengdu 610066,Sichuan);AN ANALYSIS ON THE INORGANIC ELEMENTS OF THE CODONOPSIS PILOSULA[J];JOURNAL OF SICHUAN NORMAL UNIVERSITY(NATURAL SCIENCE);1995-02
9 Li Ping China Pharmaceutical University , Nanjing 210009 Xue Can Nanjing Netsucceed Network Corporation, Nanjing 210029;A Tentative Remark on Establishment of Artificial intelligence system for quality evaluation of Chinese medicinal materials via characteristic fingerprinting of multi-data[J];World Science and Technology;2001-04
10 Zhang Ying(Institute of Chinese Materia Medica,Chinese Academy of Traditional Chinese Medicine,Beijing100700,China)Cao Hui (National Engineering Research Center for Modernization of Traditional Chinese Medicine,Zhuhai Municipality519020,Guangdong,China);The Latest Progress in Study of DNA Molecular Marker Used in Quality Evaluation of Chinese Medicinal Materials[J];;2003-01
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 DENG Feng,MO Jie-li,CHEN Hao-an(Guangdong institute for Drug Control,Guangzhou,Guangdong 510180,China);Identification of Exocarpium Citri Grandis by inter-simple sequence repeat markers[J];Journal of Guangdong Pharmaceutical College;2009-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
2 Lu Guangyuan\ Yang Guangsheng\ Fu Tingdong (Huazhong Agricultural University, Wuhan 430070);Silver-Stained Aflp- A Novel Assay for DNA Fingerprinting in Brassica napus[J];Journal of Huazhong Agricultural;2001-05
3 Li Jia; Shen Bingzhang; Han Jixiang; Gan Li (Huazhong Agricultural University, Wuhan 430070);A EFFECTIVE PROCEDURE FOR EXTRACTING TOTAL DNA IN RAPE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1994-05
4 ZHANG Jun, WU Yao ting, GUO Wang zhen, ZHANG Tian zhen (Agronomy Department, Nanjing Agricultural University; Key Laboratory of Crop Germplasm and Breeding, Ministry of Agriculture, Nanjing 210095, China);Fast Screening of Microsatellite Markers in Cotton with PAGE/silver Staining[J];ACTA GOSSYPII SINICA;2000-05
5 Wu Ningfeng 1) ,Li Rugang 1) ,Wu Xiaoming 2) ,Zhu Li 1) ,Fan Yunliu 1) ,Qian Xiuzhen 2) 1) Biotechnology Research Center,Chinese Academy of Agricltural Sciences,Beijing 100081 2) Institute of Oil Crop,Chinese Academy of Ag;RAPD molecular markers and genetic diversity among 40 cultivars of Brassica napus in China[J];CHINESE BIODIVERSITY;1997-04
6 Liu Houli (Laboratory of Genetics and Plant Breeding,Department of Agronomy,Central China Agricultural College);ORIGIN AND EVOLUTION OF RAPESEEDS[J];Acta Agronomica Sinica;1984-01
7 LIN Zhong-Xu,ZHANG Xian-Long~①,NIE Yi-Chun (National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China);Evaluation of Application of a New Molecular Marker SRAP on Analysis of F_2 Segregation Population and Genetic Diversity in Cotton[J];Acta Genetica Sinica;2004-06
8 Meng Jinling;A.Sharpe;C.Bowman;Tian Zhihong;Fu Tingdong;Qian Xiuzhen;D.Lydiate(State Key Laboratory of Crop Genetic improvement,Huazhong Agricultural University, Wuhan 430070)(Oil Crops Institute,Chinese Academy of Agricultural Sciences,Wuhan 430072)(Camb;Genetic Diversity of Brassica napus Detected with RFLP Markers[J];ACTA GENETICA SINICA;1996-04
9 WANG Feng-ge,ZHAO Jiu-ran,GUO Jing-lun,LIU Long-zhou(Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing100089,China);Series of Research on Establishing DNA Fingerprinting Pool of Chinese New Maize Cultivars Ⅰ.The Establishment of a Standard SSR System Fitting for Maize Cultivars' Identification[J];Journal of Maize Sciences;2003-01
10 Xiao Bingguang Lu Jiangping Lu Xiuping Bai Yongfu Wang Shaokun Li Tianfei China Tobacco Breeding Research (Southern) Center,Yuxi 653100;The classification of flue cured tobacco varieties using RAPD analysis[J];ACTA TABACARIA SINICA;2000-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved