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《Molecular Plant Breeding》 2008-01
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Optimization of TRAP-PCR Reaction System and Analysis of P-glycoprotein Gene in Rice

Zhu Zhikai Fang Liangjun Zhao Qianting Deng Yanhua Su Huanjie Laboratory of Molecular Plant Breeding, College of Agronomy, Guangdong Ocean University, Zhanjiang, 524088  
The factors that might influence TRAP-PCR, concentration of genomic DNA template, dNTP mixture, Taq DNA polymerase, primers, were optimized in this paper, and the advanced reaction system for TRAP-PCR in rice were established, that is total 20 μL reaction volume including 80 ng genomic DNA template, 0.25 mmol/L dNTP mixture, 0.75 U Taq DNA polymerase, 5 ng/μL arbitrary and 7.5 ng/μL specific primer. We also isolated a fragment of P-glycoprotein gene and then sequenced, which would be a basis for our further study.
【Fund】: 广东省科技攻关项目(2002B2120401);; 广东省教育厅科技攻关项目(2006B20101013)资助
【CateGory Index】: S511
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