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《Molecular Plant Breeding》 2008-01
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Cloning of Potato Inorganic Pyrophosphatase cDNA and Construction of Antisense Plant Expression Vectors

Liu Haiying 1 Li Youzhong 1 Liu Na 1 Si Huaijun 1,2 Wang Di 1 1 Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070  
Total RNA was extracted from leaves of potato cultivar Atlantic using Trizol regent. Potato inorganic pyrophosphatase (PPase) gene cDNA was amplified using the reverse transcription polymerase chain reaction (RT-PCR) method and reversely cloned into pBluescript SK+ vector. The recombinant plasmid was identified by enzyme digestion. The sequence analysis showed that the PPase cDNA was 673 bp with 636 bp open reading frame (ORF) encoded a 211-amino acid polypeptide, which is 97.62% homology to the published sequence. The antisense PPase gene was inserted into expression vectors pBI121, pBIC and pBIrd to construct plant expression vectors, promoters of which are the constitutive promoter CaMV 35S, tuber-specific expression promoter CIPP and low temperature-inducible promoter rd29A, respectively, which would be applied to develop the potato germplasms with the long or short periodic characteristics of tuber dormancy.
【Fund】: 国家自然科学基金项目(30571182);; 甘肃省人事厅人才工程项目资助
【CateGory Index】: S532
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【References】
Chinese Journal Full-text Database 2 Hits
1 Zhang Ning 1 Si Huaijun 1,2* Liu Na 2 Li Youzhong 2,3 Liu Haiying 2,4 Wen Gang 1,2 Wang Di 2 1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070; 2 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070; 3 Center for Molecular Agro-biotechnology and Breeding, Xinjiang Academy of Agri- cultural and Reclamation Science, Shihezi, 832000; 4 Potato Engineering Department, Wulanchabu Vocational College, Wulanchabu, 012000;Expression in Escherichia coli and Protein Characteristic Analysis of cDNA Encoding Inorganic Pyrophosphatase of Potato[J];Molecular Plant Breeding;2008-06
2 Zhang Chunfeng 1 Zhang Ning 2 Wang Li 2 Jiang Hanyu 2 Si Huaijun 1,2Wang Di 1 1 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Lanzhou, 730070; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070;Effect of Transgenic PPase Gene on Potato Inorganic Pyrophosphatase Activity and Inorganic Phosphorus Content[J];Molecular Plant Breeding;2009-03
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1 LI Xiao yuan,ZENG Ri zhong,XIAO Xian zhou(Rubber Research Institute /Ministry of Agriculture Key Lab for Tropical Corps Physiology, CATAS,Danzhou 571737,Hainan,China);Advances on the Research of Pyrophosphatase in Plants[J];Life Science Research;2004-S2
【Co-citations】
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1 ZHANG Lili1,LIAN Yong2,CHEN Yili1(1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China; 2.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Improvement on silver staining method of potato protein[J];Journal of Northeast Agricultural University;2007-04
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