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《Molecular Plant Breeding》 2008-01
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Establishment and Optimization of SRAP and ISSR Marker System in Citrus

Wu Xin 1,2,3 Lei Tiangang 2,3 He Yongrui 2,3 Liu Xiaofeng 2,3 Xu Lanzhen 2,3 Peng Aihong 2,3 Chen Shanchun 2,3 1 College of Horticulture & Landscape Designing, Southwest University, Chongqing, 400716; 2 Citrus Research Institute, Southwest University, Chongqing, 400712; 3 National Citrus Cultivar Improvement Center of China, Chongqing, 400712  
Here we have established SRAP-PCR and ISSR-PCR system in Citrus by optimization of PCR reaction program, reaction system (template DNA, reaction volume, Mg2+, dNTP, Taq DNA polymerase and primer) and electrophoresis detection experiments. Based on these reaction systems, a mass of primers have been screened. In the end, we got optimum SRAP and ISSR marker systems in Citrus. SRAP-PCR system: template DNA 25 ng, Tris-HCl 10 mmol/L, KCl 50 mmol/L, Mg2+ 1.2 mmol/L, dNTP 120 μmol/L, Taq DNA polymerase 1.5 U, primer 0.4 μmol/L in 25 μL system, reaction program: initial denaturation step for 5 min at 94℃, followed by 35 cycles at 94℃ for 30 s, 47℃ for 1 min and 72℃ for 1 min, followed by a final extension step at 72℃ for 10 min; ISSR-PCR system: template DNA 25 ng, Tris-HCl 10 mmol/L, KCl 50 mmol/L, Mg2+ 2.0 mmol/L, dNTP 200 μmol/L, Taq DNA polymerase 1 U, primer 0.8 μmol/L in 25 μL system. 24 pairs of SRAP primers and 13 ISSR primers, with high stability and polymorphism, has been obtained.
【Fund】: 科技部科研院所社会公益研究专项(2004DIB45147);; 重庆市科技攻关重点项目(CSTC 2006AB1009);; 国家863项目(2006AA100108-4-12-4)资助
【CateGory Index】: S666
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【References】
Chinese Journal Full-text Database 5 Hits
1 HUANG Yu,RONG Jun-dong,LI Feng-tao,CHEN Li-guang,LI Hong-guang,HE Tian-you,ZHENG Yu-shan(Institute of Industrial Forest,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Establishment and optimization of ISSR reaction system of Sarcandra glabra[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2009-02
2 LU Juan1,ZHANG Shao-ling1,LIU Qing-zhong2,WU Jun1,ZHANG Rui-ping1,LIAOYANG Wen-ke1,CHEN Ting1(1College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China;2Shandong Institute of Pomology,Tai'an,Shan-dong 271000 China);Optimization of SRAP-PCR system and its application in genetic diversity analysis of cherry[J];Journal of Fruit Science;2009-02
3 Qiao Yanchun 1,2 Lin Shunquan 1 Yang Xianghui 1 Liu Yuexue 1,2 1 College of Horticulture,South China Agriculture University,Guangzhou,510642;2 College of Horticulture,Shenyang Agricultural University,Shenyang,110161;Optimization of ISSR-PCR Analysis and its Application in Germplasm of Loquat(E.japonica Lindl.cv) by Uniform Design[J];Genomics and Applied Biology;2009-01
4 Wang Xuejun Liu Guojian Chang Ruifeng Han Jicheng Guo Encai Changli Institute of Polomogy, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600;Optimization of Annealing Temperature of SRAP-PCR in 5 Temperate Fruits[J];Genomics and Applied Biology;2009-03
5 LI Qian-zhong1,LIU Xiao-hong2,SU Jia-le1,CONG Yu1(1.Initute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.College of Agriculture,Yangzhou University,Yangzhou 225009,China);Extraction of Genomic DNA from Acer yangjuechi and System Optimization of SRAP-PCR[J];Jiangsu Journal of Agricultural Sciences;2009-03
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
2 Lei Tiangang1,2,3 He Yongrui1,2,3 Wu Xin2 Liu Xiaofeng1,2,3 Xu Lanzhen1,2,3 Peng Aihong1,2,3 Chen Shanchun1,2,3 1 Citrus Research Institute,CAAS,Chongqing,400712;2 Citrus Research Institute,Southwest University,Chongqing,400712;3 National Citrus Cultivar Improvement Center of China,Chongqing,400712;Identification of Pummelo (Citrus grandis (L.) Osbeck) and Hybrid Citrus Cultivars using SSR Molecular Markers[J];Molecular Plant Breeding;2007-05
3 Xuan Jiping,Zhang Zhen ,Fang Jinggui ,Gao Zhihong,and Che Shengli (College of Horticulture,Nanjing Agricutural University,Nanjing,Jiangsu210095China);Construction of Apple Ccultivar IS SR Fingerprinting[J];Journal of Fruit Science;2002-06
4 WU Zi-long1, FANG Lian-yu1, WANG Jun1*, SHEN Yu-jie2 ( 1College of Forestry, Northeast University of Forestry, Harbin, Heilongjiang 150040 China; 2Research Institute of Special Wild Economic Animals and Plants, CAAS, Jilin 132109 China);Analysis of genetic relationship of 15 Vitis germplasm resources by ISSR markers[J];Journal of Fruit Science;2006-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Luo Haiyan et al.(1 Tropical Crops Genetic Resources Institutes,Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China;2 Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China);ISSR Molecular Marker and its Using[J];Anhui Agricultural Science Bulletin;2008-19
2 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
3 ZHAO Xin-liang et al (Northwest A&F University,Yangling,Shaanxi 712100);Study on Classification of Maize Inbred Lines in Henan with SRAP Marker Technology[J];Journal of Anhui Agricultural Sciences;2007-07
4 PENG Shuai et al(Institute of Life Science and Biotechnology,Bioenergy and Biomaterial Research Center,Hunan University,Changsha,Hunan 410082);Analysis of Genetic Relationship of 17 Houttuynia cordate Germplasm Resources With ISSR Marker[J];Journal of Anhui Agricultural Sciences;2007-12
5 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
6 DING Jin-ping et al (College of Agronomy,Guangxi University,Nanning,Guangxi 530003);Establishment and Optimization of SRAP Reaction Conditions for Arachis hypogaea L.[J];Journal of Anhui Agricultural Sciences;2008-10
7 ZHAO Yang et al (College of Forestry,Guizhou University,Guiyang,Guizhou 550225);Establishment of DNA Extraction and ISSR-PCR Reacting System of Tutcheria kweichowensis[J];Journal of Anhui Agricultural Sciences;2008-29
8 YI Qiong et al(Biotechnology Research Center,Kunming University of Science and Technolgy,Kunming,Yunnan 650224);Analysis on Genetic Relationships among 8 Main Pear Cultivars in Yunnan Province Based on ISSR Molecular Markers[J];Journal of Anhui Agricultural Sciences;2009-02
9 DENG Bo-tao ZHAO Fei WANG Xue-ying (College of Biological Sciences and Technology,Shenyang Agricultural University,Shenyang Liaoning 110161);Optimization of SRAP in Carrot[J];;2009-13
10 DING Wei-Dong,CAO Li-Ping ,CAO Zhe-Ming Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu Province,China;The SRAP and SCAR molecular markers for detecting germ degeneration in Ctenopharyngodon idellus[J];Acta Zoologica Sinica;2008-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Liu Liwang et al(Department of Horticulture,Nanjing Agricultural University,210095);Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1997-03
2 TIAN Ming-li,WU Xiao-bing(College of Life Sciences,Anhui Normal University,Wuhu 241000,China);Optimization of Experiment Conditions and Primer Screening With SRAP Markers for Chinese Alligators[J];Journal of Anhui Normal University(Natural Science);2008-02
3 LI Jun-min,JIN Ze-xin(Ecology Institute of Taizhou University,Linhai 317000);Optimization and screening of ISSR reaction system in endangered plant Emmenopterys henryi[J];Journal of Anhui Agricultural University;2006-04
4 MING Jun 1;ZHANG Qi-xiang 2;LAN Yan-ping 3. 1) Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China; 2) College of Landscape Architecture, Beijing Forestry University, 100083, P. R. China; 3) Institute of Agriculture System and Development, Beijing Academy of Agriculture and Forestry, 100089, P. R. China.;Core collection of Prunus mume Sieb. et Zucc.[J];Journal of Beijing Forestry University;2005-02
5 ZHANG Yu-rong~(1,2);LUO Ju-chun~1;YU Jin-xiu~2.1 College of Forestry,Beijing Forestry University,100083,P.R.China;2 Hunan Forestry Academy,Changsha,410004,P.R.China.;Genetic diversity of the endangered plant Abies ziyuanensis detected by ISSR markers.[J];Journal of Beijing Forestry University;2007-06
6 Yang Chuanping, Pan Hua, Wei Zhigang, Jiang Jing(Northeast Forestry University, Harbin 150040, P. R. China);Optimization of ISSR-PCR Reaction System for Betula platyphylla Suk[J];Journal of Northeast Forestry University;2005-06
7 LI Shan,WANG Ma li,ZHAO Gui fang * (Institute of Life Science,Biodiversity Research Center of Qinling Mts.,Northwest University,Xian 710069,China);DNA extraction and characterization of Acer miaotaiense[J];Acta Botanica Boreali-occidentalia Sinica;2001-02
8 TIAN Yi-ke~(1,2),WANG Cai-hong~2,ZHANG Ji-shu~(1*),DAI Hong-yi~2,CHU Qing-gang~3(1 Life Science College, Northwest Sci-Tech University of Agriculture and Forestry. Yangling, Shannxi 712100,China;2 Horticultural Department, Laiyang Agricultural College. Laiyang, Shandong 265200,China;3 Life Science department, Laiyang Agricultural College.Laiyang, Shandong 265200,China);A RAPD marker of apple columnar gene(Co)[J];Acta Botanica Boreali-occidentalia Sinica;2003-12
9 WANG Cai-hong ,TIAN Yi-ke ,ZHAO Jing,DAI Hong-yi,WANG Dong(Horticultural Department of Laiyang Agricultural College ,Qingdao,Shandong 266109,China;Classes of National Base,Department of Chemistry,North-Western University,Xi'an 710069,China);RAPD Analysis of the Genetic Differences among Several Cherry Varieties[J];Acta Botanica Boreali-occidentalia Sinica;2005-12
10 CAI Yu-liang~ 1,2 ,CAO Dong-wei~1,LI Shan~3,QIAN Zeng-qiang~1,ZHAO Gui-fang~ 1* (1 Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,School of Life Science,Northwest University,Xi'an 710069,China;2 Pomology Institute,College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Xi'an 710065,China;3 College of Life Science and Technology,Tongji University,Shanghai 200092,China);RAPD Analysis of Prunus avium L.Varieties and Their Rootstocks[J];Acta Botanica Boreali-Occidentalia Sinica;2006-06
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 TANG Yu-hong1,BAI Qing-wu1,LIN Jia-zhi2,LI Bo1(1.Department of Biology,Qiqihar University,Qiqihar 161006,China;2.Zhalantun Teachers′ School,Zhalantun 162650,China);ISSR analysis of the grapes in the western of the Heilongjiang[J];Journal of Science of Teachers College and University;2004-04
2 Wang Jun and He Puchao ( Institute of Spend Wild Economic Animal and Plant, GAAS, Jilin 132109; Department of Horticulture, Northwestern Agricultural University);Extraction of Genomic DNA from Vitis amurensis and Its RAPD Analysis[J];JOURNAL OF FRUIT SCIENCE;2000-02
3 QiaoYushan, Zhang Zhen, Fang Jinggui, ShenZhijun, and Zhao Mizhen (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; Research Institute of Horticulture, JiangsuA-cademy of Agricultural Sciences, Nanjing, Jiangsu 210014 China);Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm[J];Journal of Fruit Science;2003-04
4 Zhang Qinglin and Luo Zhengrong  (College of Horticulture and Forestry,Huazhong Agricultural University,Wu han,Hubei430070China);ISSR Technology and Its Applications in Fruit Trees[J];Journal of Fruit Science;2004-01
5 Zuo Kaijing Sun Jizhong Zhang Xianlong Nie Yichun Liu Jinlan Feng Chunda(National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070);Constructing a Linkage Map of Upland Cotton ( Gossypium hirsutum L.) Using RFLP, RAPD and SSR Markers[J];JOURNAL OF HUAZHONG AGRICULTURAL;2000-03
6 Cheng Yunjiang Yi Hualin Pang Xiaoming Guo Wenwu Deng Xiuxin (Huazhong Agricultural University, Wuhan, 430070,China);An efficient method for genomic DNA extraction from woody fruit plants[J];Journal of Huazhong Agricultural;2001-05
7 Qiu Yingxiong Fu Chengxin Kong Hanghui ( College of Life Sciences, Zhejiang University, Hangzhou 310029, China);Inter-simple Sequence Repeat(ISSR) Analysis of Different Cultivars in Myrica rubra[J];Journal of Agricultural Biotechnology;2002-04
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