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《Molecular Plant Breeding》 2008-01
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Optimization for SRAP-PCR System of Tobacoo Based on Orthogonal Design

Chen Wansheng Wang Yuanying Luo Chenggang Yang Aiguo Jang Caihong Fan Jingyuan Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101  
In this paper, the orthogonal design was used to optimize SRAP-PCR amplification system on tobacoo (N. tabacum L.) in four levels of five factors (Taq DNA polymerase, Mg2+, DNA template, dNTP, and primer) respectively. The data generated by SRAP-PCR were analyzed by software SPSS V13.0, and the results showed that the effects of each factor in different levels were found and the quantity of Taq DNA polymerase was the most dominant factor affected on the result of PCR. A most suitable SRAP-PCR system for tobacoo was established that is total 25 μL reaction system containing 1.0 U Taq DNA polymerase, 1.5 mmol/L Mg2+, 30.00~120.00 ng DNA template, 0.15 mmol/L dNTP, and 0.40 μmol/L primer. Further, we found out the annealing temperature and cycle number based on the optimized tobacoo SRAP-PCR reaction system, which would be 52℃for SRAP-PCR annealing temperature and 35 cycles for PCR reaction procedure. This optimized system for SRAP marker would become one of the protocols for further research in our lab.
【Key Words】： Nicotiana tabacum L. SRAP Orthogonal design Optimization
【Fund】： 国家烟草专卖局科学基金项目“烟草抗病毒育种项目”;; 基本科研业务费专项(0032007023)资助
【CateGory Index】： S572;Q943.2
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【References】
Chinese Journal Full-text Database 10 Hits
1 DENG Bo-tao ZHAO Fei WANG Xue-ying (College of Biological Sciences and Technology,Shenyang Agricultural University,Shenyang Liaoning 110161);Optimization of SRAP in Carrot[J];;2009-13
2 TAN Jiang-ping~1,ZENG Xiu-li~(2,3),LIAO Ming-an~1,QIU Li-na~1,WANG Yu-xia~2,ClREN Zhuo-ga~2 (1.Horticulture of College,Sichuan Agricultural University,Ya'an,Sichuan 625014;2.Institute of Vegetables,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lasa,Tibet 850030;3.Maize Research Institute,Sichuan Agricultural University,Ya'an,Sichuan 625014);Optimization for SRAP-PCR System and Selection of Primers on Prunusmira Koehne from Tibet[J];Northern Horticulture;2011-02
3 GUO Li-qin1;WEI Zun-zheng1;ZHANG Jin-feng1;WANG Huan1;LI Xian1;GUO Jun2.1 National Engineering Laboratory for Tree Breeding,Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing Forestry University,100083,P.R.China;2 Magutian Agricultural Service Center in Biyang County,Henan Province,463721,P.R.China.;Optimization of SRAP-PCR amplification system for Populus by uniform design[J];Journal of Beijing Forestry University;2010-02
4 ZHOU Liang-Bin1,LU Xin-Shi1,LAI Li-li2 (1.Grassland Research Institute,Beijing Forestry University,Beijing 100083,China;2.College of Pratacultural and Environmental Sciences,Xinjiang Agricultural University,Urumchi,Xinjiang Province 830052,China);Optimization for SRAP-PCR System of Medicago varia Martin. Based on Orthogonal Design[J];Acta Agrestia Sinica;2010-03
5 FU Wei1,2,GAN You-min1,WU Jia-hai2,WANG Xiao-li2,CHEN Wei2,ZHANG Jian-bo2(1.Sichuan Agricultural University,Ya'an 625014,China;2.Guizhou Pratacultural Institute,Guiyang 550001,China);Genetic diversity of Dichondra repens germplasm in southwest China by SRAP[J];Acta Prataculturae Sinica;2010-06
6 Zou Xiaoyun Zou Xiaofen Chen Lunlin Li Shuyu Zhang Jianmo Chen Zhicai Song Laiqiang Institute of Crops,Jiangxi Academy of Agricultural Sciences,Key Laboratory of Oil Crops,Jiangxi Academy of Agricultural Sciences,Nanchang,330200;Optimization for SRAP-PCR System of Peanut Based on Orthogonal Design[J];Molecular Plant Breeding;2010-04
7 Qiao Yanchun 1,2 Lin Shunquan 1 Yang Xianghui 1 Liu Yuexue 1,2 1 College of Horticulture,South China Agriculture University,Guangzhou,510642;2 College of Horticulture,Shenyang Agricultural University,Shenyang,110161;Optimization of ISSR-PCR Analysis and its Application in Germplasm of Loquat(E.japonica Lindl.cv) by Uniform Design[J];Genomics and Applied Biology;2009-01
8 XU Jian-hua1,YANG Hong-qi1,YANG Cheng2,ZHOU Ji-heng1,DUAN Feng-yun2,CHEN Xin-bo1,ZHAN You-guo1,ZHAO Shi-hao1(1.Key Laboratory of Tobacco Science and Healthy,HNAU,Changsha 410128,China;2.Technology Center Tobacco Dali Corp,Dali,Yunnan 671000,China);DNA extraction and optimization of SRAP reaction system in tobacco[J];Journal of Hunan Agricultural University(Natural Sciences);2009-03
9 CHEN Hua-zeng1,2 LI Jian2 HE Yu-ying2 LI Ji-tao2 WANG Qing-yin2(1College of Fisheries and Life Science,Shanghai Ocean University,201306)(2Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071);Optimization of SRAP-PCR system using orthogonal design and its application to genetic analysis in Fenneropenaeus chinensis[J];Progress in Fishery Sciences;2010-06
10 SHAN Xiao-zheng1,HOU Xi-lin 1,2,LI Ying1,2,WU Hai-tao1,WANG Feng1,LI Mei1(1.National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China;2.Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,China);Optimization of SRAP System and Cluster Analysis of Non-heading Chinese Cabbage (Brassica campestris L. ssp. chinensis)[J];Jiangsu Journal of Agricultural Sciences;2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Jing,HU Xiao-hui,MIAO Hua-rong,ZHANG Jian-cheng,YU Shan-lin(Shandong Peanut Research Institute,Qingdao 266100,China);Optimization of SRAP-PCR Reaction System on Peanut[J];Shandong Agricultural Sciences;2008-05
2 Chen Xueping Jiang Ping Zhang Jieyu Lu Xingxing (Hefei Institute of Economics & Technology,Anhui 230052);Investigation of Esterase Isozymogram in Germplasm Resouces[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1998-03
3 Teng Caizhu~(1,3) Diao Ying~1 Chang Fuhaosen~1 Xie Shiqing~2 Han Yonghua~3 Hu Zhongli~1(1 Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University,Wuhan,430072;2 Institute of Konjak,Yunnan Agricultural University,Kunming,650201;3 College of Life Science,Guangxi Normal University,Guilin 541004);ISSR Analyses of Relative Relationships among Germplasm Resources of Amorphophallus Blume from Yunnan Province[J];Anhui Agricultural Science Bulletin;2006-11
4 WANG Qin et al (Department of Forage and Grassland,Sichuan Agricultural University, Yaan, Sichuan 625014);Turf Quality of Wild Dichondra in Sichuan[J];Journal of Anhui Agricultural Sciences;2005-04
5 WANG Chuang et al (Henan Agriculture University, Zhengzhou,Henan 450002);Soil Characteristics and its Relationship with Tobacco Leaf Quality[J];Journal of Anhui Agricultural Sciences;2005-05
6 ZENG Bing et al (Department of Grassland Science, Sichuan Agricultural University, Yaan, Sichuan 625014);Optimal of RAPD Marker in Dactylis glomerata[J];Journal of Anhui Agricultural Sciences;2005-07
7 FEI Wei-xin et al (Anhui Science and Technology Innovation Center of Dominant Crop,Hefei, Anhui 230031);Inheritance of High Oleic Acid Content in Brassica napus by Reciprocal Crosses[J];Journal of Anhui Agricultural Sciences;2007-07
8 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
9 FU Bing et al(Biotechnology Research Center of Kunming University of Science and Technolgy,Kunming,Yunnan 650224);Genetic Diversity Analysis of 25 Tobacco Germplasm Resources by ISSR Molecular Marker[J];Journal of Anhui Agricultural Sciences;2008-03
10 QUE Sheng-quan et al(Department of Biology and Environmental Engineering,Nanchang University of Technology,Nanchang,Jiangxi 330013);DNA Extraction from Camellia oleifera and the Establishment of the Optimum Reaction System of RAPD Analysis[J];Journal of Anhui Agricultural Sciences;2008-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WU Zhi-ming1,ZENG Jin2,HU Kai-lin2,QIAO Ai-min1(1.College of Horticulture and Landscape Architecture,Zhongkai University of Agricultural and Engineering,Guangzhou,Guangdong 510225;2.College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510640);Optimization of SRAP-PCR System for Pepper Using Orthogonal Design[J];Northern Horticulture;2010-11
2 HOU Yu-rong,AN Sha-zhou,LIU Dong,WANG Wei,XU Cai-qing(College of Grassland and Environment Science,Xinjiang Agricultural University;Key Laboratory of Grassland Resources and Ecology of Xinjiang,Xinjiang Urumqi 830052,China);Influence of differently geographical populations and terrains on genetic diversity of Seriphidium transiliense[J];Pratacultural Science;2011-01
3 ZHANG Jing-yuan,PENG Yan,LUO Yan,MA Xiao (Department of Grassland,Sichuan Agricultural University,Ya’an 625014,China);Genetic diversity in white clover germplasm detected by SRAP markers[J];Acta Prataculturae Sinica;2010-05
4 ZHONG Cong-lai,DING Gui-jie*,SHEN Ling(Institute of Silveculture and Ecology,Guizhou University,Guiyang,Guizhou 550025,China);Optimization of SRAP-PCR Amplification System of Jatropha curcas[J];Guizhou Agricultural Sciences;2010-04
5 XU Xiang-li,LU Xiu-ping*(Yunnan Academy of Tabacco Agriculture Sciences,Yuxi,Yunnan 653100,China);Application of molecular assisted selection in modern tobacco breeding[J];Journal of Hunan Agricultural University(Natural Sciences);2010-03
6 LI Chuan-dai1,2,CHEN Fei1,2,WANG Tian-di1,2,LIU Guo-min1,2(1.Key Laboratory of Tropical Biological Resources,MOE,Hainan University,Haikou 570228,China;2.Kudingcha Research Institute of Hainan University,Haikou 570228,China);Establishment and Optimization of ISSR-PCR Reaction System of Ligustrum lucidum Ait.[J];Journal of Tropical Organisms;2010-02
7 ZHOU Kun-hua1,2,FANG Rong1,CHEN Xue-jun1,MIAO Nan-sheng1,WANG Wu-liang3 (1.Vegetable and Flower Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;2.The Key Laboratory of Oil Crop,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;3.Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China);Optimization of SRAP-PCR System in Pepper Using Orthogonal Design and Selection of Primers[J];Acta Agriculturae Universitatis Jiangxiensis;2010-03
8 Zhao Yijie1, Wang Jiangbo2, Zhang Xiaohong1, Shi Weishu2, Lin Hang1 1 Fuzhou Institute of Agricultural Sciences, Fuzhou, Fujian 350018, China; 2 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;ISSR Molecular Identification of Eriobotrya japonica (Thunb.) Lindl. 'Dong Huzao'[J];Chinese Journal of Tropical Crops;2010-01
9 Jing Zange Tang Zheng Zhang Xiaoling Luo Tiankuan Liu QingZhu Shiyang Lou Jue Ye Zhen Chen Haiying(Zhenan Key Laboratory of Crop Breeding,Biotechnology Research Institution,Wenzhou Vocational College of Science and Technology,Wenzhou 325006);Optimization of SRAP-PCR System and Cultivar Molecular Identification in Broccoli(Brassica oleracea var. italica)[J];Biotechnology Bulletin;2010-12
10 Li Xia, Yu Jiqing, Li Shuhua, Yang Lin, Mu Meng (Weifang Academy of Agricultural Sciences, Weifang 261071,Shandong, China);Optimization for SRAP-PCR System ofAsparagus Officinalis L. Based on Orthogonal Design[J];Journal of Agricultural;2011-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zuo Kaijing Sun Jizhong Zhang Xianlong Nie Yichun Liu Jinlan Feng Chunda(National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070);Constructing a Linkage Map of Upland Cotton ( Gossypium hirsutum L.) Using RFLP, RAPD and SSR Markers[J];JOURNAL OF HUAZHONG AGRICULTURAL;2000-03
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