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《Molecular Plant Breeding》 2008-05
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Advances in the Research of Plant Chloroplast Genetic Transformation

Qian Xueyan 1,2 Yang Xiangdong 1 Guo Dongquan 1 Zhao Guilan 1 Wang Piwu 2 1 Biotechnology Research Center,Jilin Academy of Agriculture Sciences,Changchun,130033;2 Department of Agriculture,Jilin University of Agriculture Sciences,Changchun,130022  
Chloroplast genetic engineering offers a number of unique advantages,including high-level transgene expression,multigene engineering in a single transformation event,transgene containment via maternal inheritance,lack of gene silencing,position and pleiotropic effects and undesirable foreign DNA.More than 40 transgenes have been stably integrated and expressed via the tobacco chloroplast genome to confer desired agronomic traits or express high levels of vaccine antigens and biopharmaceuticals.In this paper,the principles and advantages of the chloroplast genetic engineering,the methods used for chloroplast transformation,and the recent advances in the field were illustrated and the application of the technology in future was also discussed.
【Fund】: 国家863计划项目(2007AA10Z189;2006AA100104-17);; 国家自然科学基金项目(30771355)资助
【CateGory Index】: Q943.2
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