Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2008-06
Add to Favorite Get Latest Update

Expression in Escherichia coli and Protein Characteristic Analysis of cDNA Encoding Inorganic Pyrophosphatase of Potato

Zhang Ning 1 Si Huaijun 1,2* Liu Na 2 Li Youzhong 2,3 Liu Haiying 2,4 Wen Gang 1,2 Wang Di 2 1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070; 2 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070; 3 Center for Molecular Agro-biotechnology and Breeding, Xinjiang Academy of Agri- cultural and Reclamation Science, Shihezi, 832000; 4 Potato Engineering Department, Wulanchabu Vocational College, Wulanchabu, 012000  
The characteristics of potato tuber dormancy and sprouting could be controlled by regulating inor- ganic pyrophosphate (PPi), which is a co-factor in tuber sugar metabolism. The prokaryotic expression vector pET-PPA was constructed by fusing potato inorganic pyrophosphatase (PPase) gene (GenBank accession No.: EF091820) with vector pET-28a, and introduced into Escherichia coli BL21 (DE3). The result of SDS-PAGE analysis showed that the PPase gene could express in E. coli. The analysis of biological software showed that the nucleotide sequence of the PPase gene shared 89%~97% identity to Solanum, and 77%~80% identity to other plants. Phylogenetic tree based on the nucleotide sequence alignment showed the PPase gene was closed to Solanum and Beta vulgaris species. The PPase protein had several phosphorylation sites, and without trans- membrane region, and alpha helix with 63 amino acids and extended strand with 38 amino acids, which was mainly a kind of protein existed in cytoplasm and mitochondrial matrix space.
【Fund】: 国家自然科学基金项目(30571182);; 甘肃省人事厅人才工程项目资助
【CateGory Index】: S532
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 Yang Tao 1,2 Zhang Ning 1 Li Liang 2,3 Liu Haiying 4 Si Huaijun 1,2 Wang Di 2,3 1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070; 2 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Lanzhou, 730070; 3 College of Agronomy, Gansu Agricultural University, Lanzhou, 730070; 4 Potato Engineering Department, Wulanchabu Vocational College, Wulanchabu, 012000;Cloning and Bioinformatic Analysis of Soluble Starch Synthase SSⅢ Gene in Potato[J];Molecular Plant Breeding;2009-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Liu Haiying 1 Li Youzhong 1 Liu Na 1 Si Huaijun 1,2 Wang Di 1 1 Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070;Cloning of Potato Inorganic Pyrophosphatase cDNA and Construction of Antisense Plant Expression Vectors[J];Molecular Plant Breeding;2008-01
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 Zhang Chunfeng 1 Zhang Ning 2 Wang Li 2 Jiang Hanyu 2 Si Huaijun 1,2Wang Di 1 1 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Lanzhou, 730070; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070;Effect of Transgenic PPase Gene on Potato Inorganic Pyrophosphatase Activity and Inorganic Phosphorus Content[J];Molecular Plant Breeding;2009-03
2 Wen Yikai 1 Du Honghui 2 Liu Bailin 2 Zhang Ning 2 Si Huaijun 1,2 Wang Di 1 1 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Lanzhou, 730070; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070;Effect of Transgenic PPase Gene on Dormancy-related Physiological Characters of Potato Tuber[J];Molecular Plant Breeding;2010-02
3 ZHANG Yong-fang(Schoole of Agronomy,Shanxi Datong University,Datong Shanxi,037009);Advances of Research on The Potato Molecular Breeding[J];Journal of Shanxi Datong University(Natural Science Edition);2010-03
China Proceedings of conference Full-text Database 1 Hits
1 Song Bo-tao Liu Jun (Huazhong Agricultural University,Wuhan 430070);DEVELOPMENT OIN RESEARCH ON REGULATION OF STACH-SUGER METABOLISM OF POTATOES[A];[C];2002
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wu Lu-guang(Hen?n-Agricultural University);PRELIMINARY RESEARCH ON FUNCTION OF PEROXIDASE IN ACER MONO SEED DORMANCY TO GERMINATION[J];Journal of Northeast Forestry University;1987-06
2 Liu Haiying 1 Li Youzhong 1 Liu Na 1 Si Huaijun 1,2 Wang Di 1 1 Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070;Cloning of Potato Inorganic Pyrophosphatase cDNA and Construction of Antisense Plant Expression Vectors[J];Molecular Plant Breeding;2008-01
3 Zhang Chunfeng 1 Zhang Ning 2 Wang Li 2 Jiang Hanyu 2 Si Huaijun 1,2Wang Di 1 1 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Lanzhou, 730070; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070;Effect of Transgenic PPase Gene on Potato Inorganic Pyrophosphatase Activity and Inorganic Phosphorus Content[J];Molecular Plant Breeding;2009-03
4 WANG Xin-yan,HE Hui-ling,LIANG Guan-sheng,ZHAO Chun-xiang (College of Agriculture and Landscape Architecture,Zhongkai University of Agricultural Technology,Guangzhou,Guangdong 510225,China);Study on the dormancy of mume cultivars in southern subtropical area of China[J];Journal of Fruit Science;2007-03
5 WANG Peng,LIAN Yong,JIN Liping(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing100081,China);The Research on the Regulation of Enzymes During Dormancy and Dormancy Releasing[J];Acta Agriculturae Boreall—sinica;2003-01
6 Meng Meilian, Liu Mengyun, Men Fuyi and Zhang Hongzhi ( Inner Mongolia College of Agriculture and Animal Husbandry, Huhhot 010018 );THE EFFECT OF GA3 AND ABA ON THE FORMATION OF POTATO TUBER[J];Chinese Potato;1994-03
7 WANG Peng,LIAN Yong,JIN Li-ping (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081);THE PRIMARY RESEARCH OF THE DOMANT MECHANISM ON POTATO TUBER MERISTEM[J];Chinese Potato;2002-04
8 GUO Hua-chun, SHA Ben-cai, YU Yang (Root and Tuber Crop Research Institute, Yunnan Agricultural University, Kunming 650201, China);GROWTH AND YIELD OF POTATO PLANTS AS INFLUENCED BY GIBBERELLIN TREATED SEED TUBERS[J];Chinese Potato;2003-06
9 YANG Bo-yun,LIAN Yong,ZHANG Min-hua,LUO Li-ping,CAI Qi-ying(Life Science and Food Technology College of Nanchang University,Nanchang330 047,China);DYNAMIC CHANGES OF SEVERAL ENDOHORMONES DURING DORMANCY OF POTATOS[J];Chinese Potato;2004-06
10 Meng Meilian; Liu Mengyun; Men Fuyi and Chen Youjun(Inner Mogogolia Institute of Agriculture and Animal Husbandry, H uhhot 010018);STUDY ON THE CONTENT OF INTERNAL GA_3 AND ABA IN GROWING POTATO TUBER[J];CHINESE POTATO JOURNAL;1996-01
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Xiao yuan,ZENG Ri zhong,XIAO Xian zhou(Rubber Research Institute /Ministry of Agriculture Key Lab for Tropical Corps Physiology, CATAS,Danzhou 571737,Hainan,China);Advances on the Research of Pyrophosphatase in Plants[J];Life Science Research;2004-S2
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved