Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2009-01
Add to Favorite Get Latest Update

Feasibility of the Chloroplast 5S rDNA ITS and matK Gene Sequence to the Phylogenetic Relationships in the Genus of Phyllostachys

Li Lubin1 Liu Lei2 Yuan Jinling 3 Dong Yinmao 2 Hu Tao 1 Miao Kun 1 He Congfen 2 1 Key Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091; 2 Beijing Key Lab of Plant Resources Research and Development, College of Chemistry and Environmental Engineering, Beijing Technology and Business University, Beijing, 100037; 3 Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, 311400  
The method of CTAB was used to extract 37 species bamboo genomic DNA. The primers were designed based on the chloroplast 5S rDNA internal transcribed spacer (ITS ) and matK gene sequence which were published in the GeneBank. The PCR product of 5S rRNA ITS is about 450 bp and then was sequenced directly. The se-quencing results were analysed by software DNAMAN and showed that the 37 species had the same length except Phyllostachys shuchengensis had a variable site (compared with other species among Phyllostachys) in the 392nd (A→C). The results indicate that 5S rDNA ITS sequence in 37 species bamboo was very conservative and it could not provide enough information for the relationship of the species among Phyllostachys. PCR product of matK se-quence amplified from 3 species bamboo genomic DNA was sequenced respectively and it is about 1 500 bp. Based on multiple sequence alignment of matK sequence, RFLP (restriction fragment length polymorphism) site (BstNⅠ) was discovered. After all the PCR products of matK sequence were digested by BstNⅠ, three species Phyllostachys meyeri, Phyllostachys parvifolia and Phyllostachys angusta were branched from the others in Phyl lostachys in the 938th. The results indicated that the matK gene in Phyllostachys is also conservative, and it can not provide enough information for evolutionary studies under Phyllostachys. The chloroplast 5S rDNA ITS gene and matK gene have no value for the phylogenetic relationships in the genus of Phyllostachys, but they could be used to study the phylogenetic relationships of above the genera and faster rate of evolution in the genera.
【Fund】: 中国林科院亚林所基本科研业务费(RISF6807);; 国家林业局植物新品种测试指南及已知品种数据库(2009005)资助
【CateGory Index】: S795
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 9 Hits
1 YAN Ping1,2, JIAO Xu-Wen1, PANG Qi-Hua3, SHEN Yan-Jing1, ZHAO Shu-Jin2 (1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China) (2.Guangzhou Liuhuaqiao Hospital, Guangzhou 510010,China)(3.College of life science of South China Normal University, Guangzhou 510631, China);Study on the Genetic Variation of Polygonum multiflorum Thunb species by sequences analysis of chloroplast matK gene[J];Modern Food Science and Technology;2007-08
2 SHI Ming-Wang~(1,2),PANG Yan-Jun~1,WANG Xiu-Qiang~1,YANG Yong-Hua~1(1.Stat Key Laboratory of Pharmaceutical Biotechnology,Plant Cess and MolecularBiology Laboratory,School of Life Science,Nanjing University,Nanjing 210003,Jiangsu,China;2.Henan Institute of Science and Technology,Xinxian 453003,Henan,China);Progress and Perspective in the Research of Molecular Biology in Bamboo[J];Nonwood Forest Research;2006-02
3 Li Shuxian\ Yin Tongming\ Zou Huiyu\ Ding Yulong\ Huang Minren(Nanjing Forestry University\ Nanjing210037);PRELIMINARY STUDY ON MOLECULAR SYSTEMATICS OF BAMBOO BY SSR PRIMERS DERIVED FROM RICE[J];Scientia Silvae Sinicae;2002-03
4 TIAN Xin,LI De Zhu (Kunming Institute of Botany,Chinese Academy of Sciences, Kunming 650204,China);Application of DNA Sequences in Plant Phylogenetic Study[J];Acta Botanica Yunnanica;2002-02
5 Gao Suping~1,Ren Yanjun~ 1,2 ,and Chen Qibing~1 ( ~1College of Forestry and Horticulture, Sichuan Agricultrual University, Ya'an, Sichuan 625014, China; ~2Academy of Southwest Exploration and Design, Chengdu, Sichuan 610066,China);RAPD Analysis of Genetic Relationships among the 23 Ornamental Bamboo Species in Phyllostachys[J];Acta Horticulturae Sinica;2006-03
6 ;A Rapid Method for Isolation DNA from Small Amount Leave of Bamboo[J];World Bamboo and Rattan;2005-04
7 Wang Zhengping(University of Nanjing, Nanjing 210008);A Proposal Concerning a System of Classification of Bambusoideae from China[J];JOURNAL OF BAMBOO RESEARCH;1997-04
8 Lin Xinchun 1,2 Fang Wei 1(1.Zhejiang Key Laboratory of Modern Silviculture Technology, Zhejiang Forestry College, Lin'an 311300,Zhejiang, China; 2.College of Resources and Enviroment, Beijing Forestry University, Beijing 100080, China);Advances in the Research of Bambuseae Taxonomy[J];Journal of Bamboo Research;2005-01
9 Zhang Shan Tang Dingqin(Key Lab for Modern Silvicultural Technology of Zhejiang Province,Zhejiang Forestry College,Linan 311300,Zhejiang,China);A Review on Application of DNA Marker in Bamboos and Its Limits[J];Journal of Bamboo Research;2007-01
Chinese Journal Full-text Database 10 Hits
1 GAO Qing-bo et al (Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai 810001);Preliminary Studies on ITS Sequences of nrDNA from Rhodiola alsia[J];Journal of Anhui Agricultural Sciences;2009-01
2 BAI Rui1,HE Cong-fen1,DONG Yin-mao1,LI Lu-bin2,LIU Lei1,PENG Zhen-hua2(1.Beijing Key Lab of Plant Resources Research and Development,College of Chemistry and Environmental Engineering,Beijing Technology and Business University,Beijing 100037,China;2.Key Lab of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China);A Preliminary Analysis of Phylogenetic Relationships in Orchidaceae Based on cp rRNA ITS Sequence Data[J];Northern Horticulture;2008-01
3 TANG Wen-li~1;PENG Zhen-hua~(1,2);GAO Jian~1.1 Key Laboratory of Bamboo and Rattan Science & Technology of State Forestry Administration,International Centre for Bamboo and Rattan,Beijing,100102,P.R.China;2 Research Institute of Forestry,Chinese Academy of Forestry,Beijing,100091, P.R.China.;Cloning and sequence analysis of the light harvesting complex Ⅰ gene(Lhca1) from three kinds of Phyllostachys and structure prediction of the protein[J];Journal of Beijing Forestry University;2008-04
4 CHE Wei-ai,ZHANG Tie-ming(College of Life Science,Capital Normal University,Beijing 100037,China);Research Advances in Bryophyte Phylogenetic Research[J];Acta Botanica Boreali-Occidentalia Sinica;2006-06
5 ZHENG Rong~1,2,ZHENG Wei-peng~2,FAN Wei~3(1.College of Resources and Environment,Beijing Forestry University,Beijing 100083,China;2.Fujian Academy of Forestry,Fuzhou,Fujian 350012,China;3.Zhejiang Forestry College,Hangzhou,Zhejiang 311300,China);Application of DNA Molecular Markers in Studying Bamboo Toxonomy[J];Journal of Fujian Forestry Science and Technology;2006-03
6 HUANG Sheng-xiong,HU Shang-lian,SUN Xia,JIANG Yao(Life Science and Engineering College,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China);Cloning and Sequence Analysis of β-Tubulin Gene Fragments of Neosinocalamus affinis[J];Journal of Fujian Forestry Science and Technology;2009-01
7 JIANG Fan1,3,GAO Hui-ying1,2,CHEN Xiu-ping1,3,ZHENG Shao-quan1,3(1Fujian Fruit Breeding Engineering Technology Research Center for Longan & Loquat,Fuzhou,Fujian 350013 China;2Agriculture College,Hebei Engineering University,Handan,Hebei 057150 China;3Fruit Research Institute,Fujian Academy of Agricultural Science,Fuzhou,Fujian 350013 China);Analysis of ITS sequences of rDNA in Dimocarpus plants[J];Journal of Fruit Science;2008-02
8 CAO Qing-qin1, ZHEN Zhen2, JIANG Jie1, LIU Yu-fen1, FENG Yong-qing2, QIN Ling2* (1Department of Biotechnology, 2Department of Plant Science and Technology, Beijing Agricultural College, Beijing 102206 China);Chloroplast DNA analysis technology and its application in Castanea[J];Journal of Fruit Science;2008-03
9 LI Yi~1, ZHANG Xiao-lei~1, JIANG Zhao-hui~2, WU Xiao-Li~2, XIE Yuan~1, SHAN Ke-ren~1(1. The Key Laboratory of Molecular Biology of Guiyang Medical College, Guiyang 550004, China; 2. Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China);The Isolation of Total DNA of Gastrodia.elata and Its Identification with PCR Amplification[J];Journal of Guiyang Medical College;2005-04
10 YANG Li-en~(1,2),JIN Jie~1,ZHU Jian-yi~1,HAN Xiao-lei~1,MU Xin-wu~(1,3),YAO Chun-yan~(1,3) XU Pu~1 (1.College of Biology and Food Engineering,Changshu Institute of Technology,Changshu 215500,China; 2.Department of Cell Biology,Suzhou University,Suzhou 215123,China; 3.College of Life Science,Nanjing Normal University,Nanjing 210046,China);Taxonomy of Porphyra(Rhodophyta,Bangiales) based on rbcL Sequences from the thallus[J];Journal of Changshu Institute of Technology;2009-08
China Proceedings of conference Full-text Database 1 Hits
1 Mu Shaohua Gao Zhimin Guo Qirong Li Xueping Ma Yanjun (SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan Beijing 100102);Genetic diversity of Indocalamus determined by chloroplast DNA sequence[A];[C];2009
Chinese Journal Full-text Database 10 Hits
1 Liu Mengjun; Shang Xunsheng(Hebei Agricultural University, Baoding 071001 )Teng Zhongcai(Dept of Biology, Baoding Teachers College, Baoding 071000);Wild Fruit Germplasm of China[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1998-01
2 Liu Mengjun(Research Center of Chinese Jujube, HAU, Baoding 071001)Shin Yong - UK ;Yae Byeong - WOO(National Horticultural Research Institute, Suwon 440310, Rep. of Korea);Factors Influencing RAPD Analysis in Genus Malus[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1998-04
3 XU Feng xia (South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China);The systematic relationship of the tribe Magnolieae and Michelieae ——based on matK sequence analysis[J];Acta Botanica Boreali-occidentalia Sinica;2003-07
4 TIAN Yi-ke~(1,2),WANG Cai-hong~2,ZHANG Ji-shu~(1*),DAI Hong-yi~2,CHU Qing-gang~3(1 Life Science College, Northwest Sci-Tech University of Agriculture and Forestry. Yangling, Shannxi 712100,China;2 Horticultural Department, Laiyang Agricultural College. Laiyang, Shandong 265200,China;3 Life Science department, Laiyang Agricultural College.Laiyang, Shandong 265200,China);A RAPD marker of apple columnar gene(Co)[J];Acta Botanica Boreali-occidentalia Sinica;2003-12
5 CHEN Xi1,TANG Geng-guo1,ZHENG Yu-hong2,WANG Lei-hong1(1 College of Forest Resources and Environment,Nanjing Forest University,Nanjing 210037,China;2 Institute of Botany,Jiangsu Province & Chinese Academy of Sciences,Nanjing Botanical Garden (Mem.Sun Yat-sen),Nanjing 210014,China);RAPD Analysis of Genetic Diversity of Malus baccata(L.) Borkh[J];Acta Botanica Boreali-Occidentalia Sinica;2008-10
6 Zhu Zhikai Fang Liangjun Zhao Qianting Deng Yanhua Su Huanjie Laboratory of Molecular Plant Breeding, College of Agronomy, Guangdong Ocean University, Zhanjiang, 524088;Optimization of TRAP-PCR Reaction System and Analysis of P-glycoprotein Gene in Rice[J];Molecular Plant Breeding;2008-01
7 GAO Yuan, LIU Feng-zhi*, CAO Yu-fen, WANG Kun (Research Institute of Pomology, CAAS, Xingcheng, Liaoning 125100 China);Analysis of genetic relationship for Malus germplasm resources by SSR markers[J];Journal of Fruit Science;2007-02
8 Ku, Tsue Chih (Institute of Botany, Academia Sinica, Beijing)andLi, Chao Luan (Chengdu Institute of Biology, Academia Sinica, Chengdu);STUDY ON MALUS DOUMERI AND ITS RELATIVES[J];Guihaia;1990-01
9 ZHU Huifen, ZHANG Changqin*, GONG Xun( Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China );A summary on plant introduction and acclimatization research[J];Guihaia;2003-01
10 HAN Lei1,XU Ji-zhong1,LI Zhen-xia2,SUN Ye-hong1,ZHANG Yuan1,SHAO Jian-zhu1(1.College of Horticulture,Agricultural University of Hebei,Baoding 071001,China;2.Department of Life Science,College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang 050018,China);Identification of Apple Rootstocks with AFLP Molecular Markers[J];Acta Agriculturae Boreali-Sinica;2008-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIAO Guo-hua (Sanming Instituite of Forestry Science, Sanming, Fujian 365000, China);RAPD fingerprinting maps in individuals of yellow and white bamboo shoot[J];Fujian Journal of Agricultural Sciences;2003-01
2 Ma Hongli* Zhang Shubiao Institute of Genetics & Crop Breeding, Fujian Agriculture and Forestry University, Fuzhou, 350002;Advances in Producing Rice Male Sterility by Bioechnology[J];Molecular Plant Breeding;2003-Z1
3 Peng Siyi and Zhang Jihong(Forest Bureau of Liling City. Hunan Province. 412200);Redsheath Bamboo.Advancing Shoot Sprouting and Increasing Shoot ProductionBy Ground Cover with Rice Husk[J];Economic Forest Reseaches;1997-03
4 Xie Bixia, Zhong Haiyan, Xie Tao, Li Anping, Jiang Nianqiong South Central Forestry University, Zhuzhou, Hunan 412006;Dietary Fiber of Bamboo Shoots and Its Health Functions[J];ECONOMIC FOREST RESEACHES;2000-02
5 XU Liu yi 1, ZHONG Jia duo 2, HU Shi rong 3(1. Forest Research Institute of Anhui Province, Hefei, Anhui 230003, China; 2. Forest Bureau of Tongling County, Anhui, 244100, China; 3. Forest Bureau of Jing County, Anhui, 242500, China);The Effect of Rare Earth Application on the Growth of Bamboo Shoots[J];Economic Forest Reseaches;2000-04
6 LI An-Ping, XIE Bi-Xia, ZHENG Shi-Hong, CAO Qing-Ming, HUANG Liang (Green Food Research Institute of Central South Forestry University, Zhuzhou, Hunan, China);Study on Drying Technique of Bamboo Shoot[J];Economic Forest Researches;2004-02
7 XIA You geng,XU Er na,XU Cui hua,WANG Guo gui// Journal of Jiangsu Forestry Science & Technology —2000,27(3):44~48;Making progress on bamboo research of China[J];JOURNAL OF JIANGSU FORESTRY SCIENCE & TECHNOLOGY;2000-03
8 Yang Guangyao 1 Zhao Qiseng 2(1College of Forestry, JAU, Nanchang330045;2Nanjing Forestry University, Nanjing210037);Phylogenetic Relationships among Five Pleioblastus Species (Bambusoideae) from China and Japan Based on Random Amplified Polymorphic DNA[J];Acta Agriculturae Universitis Jiangxiensis;2000-04
9 ZHUO Ren-ying~1,LIU Xiao-guang~2(1.The Research Institute of Subtropical Forestry, CAF, Fuyang 311400, China;2.Agricultural Science Institute of Tangshan City, Hebei Province,Tangshan 063001,China);Factors Effecting Transgenic Breeding of Dendrocalamus latiflorus[J];Acta Agriculturae Universitis Jiangxiensis;2004-04
10 PU Xiao-Ian, LI Peng, DU Fan (1. Nanjing Forestry University, Jiangsu Nanjing 210037, China; 2. Southwest Forestry College,Kunming 650224, China);Extraction of Genomic DNA and Optimization of RAPD Condition for Dendrocalamus sinicus Chia et J. L. Su.[J];Journal of Kunming University of Science and Technology;2003-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved