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《Molecular Plant Breeding》 2009-02
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Cloning and Characterization of MsChiⅣ Gene from Medicago sativa L.

Wang Buyun Li Cong Wang Yongxin Guan Ning Miao Lihong Forage Plants' Genetics and Breeding Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193  
A conserved sequence of the class Ⅳ chitinase from the root of Medicago Sativa L. had been cloned by RT-PCR, with primers designed according to the sequence segment of the class Ⅳ chitinase from Medicago trun-catula (GenBank accession number: AF167328). A cDNA clone encoding the class Ⅳ chitinase from Medicago Sativa L., named as MsChiⅣ (GenBank accession number: FJ487629), was obtained by rapid-amplification of cDNA ends (RACE) technology using primers designed in accordance with the conserved sequence of class Ⅳ chitinase from Medicago Sativa L.. The 3' terminal and the 5' terminal of MsChi Ⅳ gene were cloned by RACE, respectively. The sequence consisted of 1 025 bp and had an open reading frame (ORF) of 849 bp which encoded a protein of 282 amino acids including a signal peptide, chitin-binding domain and glycoside-hydrolase domain. The estimated molecular weight and isoelectric point of the putative protein were 30.5 kD and 4.66, respectively. It showed ex-tensive similarities to the known class Ⅳ chitinase of some plants. The similarity was up to 98% in the Medicago truncatula L., In addition, the analysis showed that it was a non-transmembrane protein.
【Fund】: 国家十一五科技支撑计划(2006BAD01A19);; 中国农科院院所基金(ywf-td-03)资助
【CateGory Index】: S541.9
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