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《Molecular Plant Breeding》 2009-03
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Stable Expression of Exogenous DREB1B in Transgenic Wheat Offspring

Rong Hongying 1,2 Zhang Liquan 1 Yang Fengping 1 Chen Xuqing 1 Zhang Xiaodong 1 Guo Xinmei 3 1 Beijing Agro-Biotech Research Center, Beijing Academy of Agricultural and Forestry Science, Beijing, 100097; 2 College of Life Science, Capital Normal University, Beijing, 100037; 3 College of Life Science, Qingdao Agricultural University, Qingdao, 266109  
Transgenic wheat offspring, T4 generation, with plasmid pBAC128F/R (7.0 kb), which contain rd29b (induced by drought stress) promoter driven DREB1B gene, and CaMV35S promoter plus maize (Zea mays L.) Adh1 first intron driven bar gene were studied on expression stability. Analysis of PCR, PCR-Southern and Southern blot for transgenic lines of T4 generation proved that exogenous DREB1B has been integrated into genome. And semi-quantitative RT-PCR analysis indicated that DREB1B was well expressed in part of lines. Herbicide resistance of 8 lines were more than 150 mg/L. Content of proline in leaves of T4 lines was determined and analysed. Content of proline of 4 transgenic lines (number 1, 18, 30 and 76) exhibited increasing 2~3 folds as compared to that of nontransgenic plants under drought stress. In addition, content of proline after drought treatment has been increased by 3~5 times as compared with that of non-drought treatment for the same one among the 4 transgenic lines. In drought stress condition, field yield data analysis of T4 generation indicated that yield of 4 transgenic plants (number 30, 51, 70 and 76) were obviously higher than negative control. Our research suggested that enhancement expression of alien transcriptional factors DREB1B driven rd29b promoter into wheat was an effective way to improve its drought tolerance ability.
【Fund】: 北京市重大转基因育种平台项目(D08070503010000);; 国家转基因生物新品种培育重大专项课题(2008ZX08002-002)共同资助
【CateGory Index】: S512.1
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