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《Molecular Plant Breeding》 2010-02
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Cloning and Bioinformatics Analysis of Chintnase ClassⅢ Gene in Medicago sative

Zhang Wenjuan Li Cong * Wang Yongxin Miao Lihong Forage Plants' Genetics and Breeding Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193  
The cDNA of chitinase ClassⅢ gene (GenBank accession: AY294484.1) from Medicago sative was cloned by RT-PCR, with primers designed according to the sequence of chitinase ClassⅢ of Medicago trucatula, It was named as MsChiⅢ, and its GenBank accession number is FJ872918. The isoelectric point of amino acid sequence, secondary and three-dimersional structures of MsChiⅢ were analysed by bioinformatics software, and the homologue tree of chitinaseⅢ was established. The result showed that the sequence consisted of 953 bp and had an full open reading frame, which encoded a polypeptide of 301 amino acid residues. The estimated PI of ClassⅢ chitinase of Medicago sative was 8.212. The protein coded by this sequence as incision enzyme belong to chitinase family 18, dispersed in intercellular space, and the amino acid sequence included special sequence of chitinase family 18, with an amino acid sequence of LGDVDFDIE. The effects of chitinase ClassⅢ would include chitinase and lysozyme.
【Fund】: 国家十一五科技支撑计划(2006BAD01A19);; 农业部苜蓿种质资源保种专项共同资助
【CateGory Index】: S541.9;Q943.2
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 LIU Ya guang,XU Gang,YANG Qing kai (Department of Plant Protection,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);A study on relatitonship between activation of chitinase in soybean leaves and resistance of soybean to Cercospora sojina[J];Journal of Northeast Agricultural University;2003-02
2 Wang Haibo;Jin Sa;Tao Yan(Department of Biology);Insect resistance of the chitinase in Viola faba leaves to Aphis craccivore[J];;1994-03
3 Ouyang Shi-wen 1, Zhao Kai-Jun 2,Feng Lan-Xiang 1, Xie Bing-yan 1 (1 Institute of vegetables and flowers, Chinese Academy of Agricultural Sciences, Beijing 100081) (2 Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Scienc;Advances in Research of Plant Chitinases[J];Progress In Biotechnology;2001-04
4 GAO Yu, QI Dong-dong, XIE Xiang-mingBiological College of Beijing Forestry University, Beijing 100083, China;Advances in research on genetic engineering and molecular biology of plant chitinase[J];Letters in Biotechnology;2004-06
5 Nan Xiangri (Heilongjiang Academy of Agricultural Sciences, Harbin 150086);Transformation and Expression of Chitinase in Potato[J];Chinese Agricultural Science Bulletin;2006-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Ya guang,XU Gang,YANG Qing kai (Department of Plant Protection,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);A study on relatitonship between activation of chitinase in soybean leaves and resistance of soybean to Cercospora sojina[J];Journal of Northeast Agricultural University;2003-02
2 Wang Quanwei 1 Qu Min 2 Zhang Hailing 1 Xu Xiangling 1 1 Life Science and Environment Institute, Harbin Normal University, Harbin, 150080; 2 Food Engineering College, Harbin University of Commerce, Harbin, 150076;Cloning of Bean Chitinase Gene Bchi and its Expression in Transgenic Tobacco[J];Molecular Plant Breeding;2008-01
3 WANG Feng, MA Yue, GAO Xiu-yan, ZHANG Zhi-hong (College of Horticulture, Shenyang Agricultural University,Shenyang, Liaoning 110161 China);Study on the identification techniques for determining strawberry cultivar's resistance to anthracnose[J];Journal of Fruit Science;2008-04
4 SUN Duo-xin,SHANG Xun-wu,SHI Gui-ying,LI Chang-sheng,LIU Juan (College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China);Effect of four kinds of enzymes on resistance to aphide of spring wheat[J];Journal of Gansu Agricultural University;2006-05
5 JIN Hua et al(College of Life Science,Dalian Nationalities University,Dalian,Liaoning 116600);Molecular Cloning and Sequence Analysis of Class Ⅱ Chitinase Gene in Leymus chinensis[J];Journal of Anhui Agricultural Sciences;2009-31
6 ZHANG Li-li,SHI Xiao-xin,DU Guo-qiang,WANG Hui-ying(College of Horticulture,Agriculture University of Hebei,Baoding 071001,China);Research Advances on Mechanism of Disease Resistance in Plants and the Breeding in Fruit Trees[J];Acta Agriculturae Boreali-Sinica;2006-S2
7 RUAN Xiao-lei,LI Hua-ping(Lab of Plant Virology,South China Agricultural University,Guangzhou 510642,China);Cloning of a Modified Chitinase Ⅰ Gene from Tobacco and Construction of a Plant Multiple Gene Expression Vector[J];Journal of South China Agricultural University;2008-01
8 ZHANG Li-fan1,WANG Bin2,GAI Wei-ling1,WANG Xiao-jie1,QIAO Li-xian1(1.College of Life Science,QAU,Qingdao 266109,China;2.Institute of Genetics and Developmental Biology,Chinese Academy of Science);Studies on Transformation of Peanut with CH_5 B Gene Mediated by Agrobacterium tumefaciens[J];Journal of Qingdao Agricultural University(Natural Science);2008-02
9 JIN Hua,AN Xiao-wen,JIANG Guo-binCollege of Life Science,Dalian Nationalities University,Dalian 116600;Molecular Cloning and Sequence Analysis of Class Ⅱ Chitinase Gene in Leymus chinensis[J];农业科学与技术(英文版);2009-04
10 YU Yu1,2,WANG Xia-qing1,FENG Chang-hui1,LIN Zhong-xu1,ZHANG Xian-long1(1.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan,Hubei 430070,China;2.Cotton Institute,Xinjiang Academy Agricultural and Reclamation Science,Shihezi,Xinjiang 832000,China);Chromosome Mapping Fiber Specific/Enriched Genes in Cotton[J];Cotton Science;2009-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Haibo;Jin Sa;Tao Yan(Department of Biology);Insect resistance of the chitinase in Viola faba leaves to Aphis craccivore[J];;1994-03
2 Gao Bida (Department of Plant Protection,HNAU,Changsha,410128);MOLECULARBIOLOGY OF PLANT CHITINASES[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;1996-06
3 ZHOU Miaoping, LU Weizhong (Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing210014);Plant Chitinases and Its Application in Anti fungal Gene Engineering[J];JIANGSU JOURNAL OF AGRICULTURAL SCIENCES;1998-03
4 WU Qing\|Jun, ZHANG Wen\|Ji (Department of Applied Chemistry, China Agricultural University, Beijing 100094, China),ZHANG You\|Jun (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China).;Insect chitinase and its potential use in plant protection.[J];Entomological Knowledge;2000-05
5 ZHOU Si jun,LI Xi chen,LIU Zhao jun,LIU Wen ping and NAN Xiang ri (Biotechnology Research Center,Heilongjiang Academy of Agricultural Sciences,Harbin 150086);INTRODUCTION OF CHITINASE GENE FROM KIDNEY BEAN INTO POTATO BY AGROBACTERIUM MEDIATED TRANSFORMATION[J];CHINESE POTATO;2000-02
6 LAN Haiyan CHEN Zhenghua (Institute of Genetics,Chinese Academy of Sciences, Beijing, 100101, PRC; Institute of Industrial Crops,Xinjiang Academy of Agricultural Sciences,Wurumqi,830000,PRC);Advances in Plant Chitinase Research[J];LIFE SCIENCE RESEARCH;1998-03
7 CUI Ping 1,JIANG Hong-lei 2 (1.Department of Biochemistry,Shandong Educational College,Jinan 250013,China; 2.Shandong Yisui Experimental Middle School,Yishui 276400,China);Chitinase and its application in the anti-disease genetic engineering[J];JOURNAL OF SHANDONG INSTITUTE OF LIGHT INDUSTRY(NATURAL SCIENCE EDITION);2000-03
8 Wang Haibo Zhou jilun (Biology Dept. of Fudan Univ.);THE PHYSIOLOGICAL RESISTANCE STRATEGY OF Vicia faba TO Aphis craccivora's FEEDING PRESSURE[J];Acta Ecologica Sinica;1988-03
9 Ouyang Shi-wen 1, Zhao Kai-Jun 2,Feng Lan-Xiang 1, Xie Bing-yan 1 (1 Institute of vegetables and flowers, Chinese Academy of Agricultural Sciences, Beijing 100081) (2 Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Scienc;Advances in Research of Plant Chitinases[J];Progress In Biotechnology;2001-04
10 Zheng Aiping Li Ping (Rice Research Institute Sichuan Agricultural University Wenjiang 611130);Advances of Researches on Bacterial Chitinase[J];Progress In Biotechnology;2002-04
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