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《Molecular Plant Breeding》 2010-02
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Optimization of an Agrobacterium-mediated Transformation System for Wheat

Li Minghao Chen Wei Xing Liping Xiao Jin Wang Haiyan Cao Aizhong Wang Xiue National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural Uniwersity, Nanjing, 210095  
In this research, we employed the callus induced from immature embryo of wheat varieties Yangmai 15 and Alondra's to be as materials for studying the transformation efficiency on main factors of Agrobacterium-mediated for by using the transient expression frequency of gus gene. The results showed that the optimized condition for the above two varieties were different under the same condition. The best condition in Agrobacterium-mediated transformation of Yangmai 15 are: First pre-cultured without acetosyringone, then infected for 10 min with Agrobacterium tumefaciens bacterial at a concentration of OD600=0.2 and acetosyringone at a concentration of 100 μmol/L, finally co-cultured for 1 day at the temperature of 25℃; and under the similar condition, the most transient expression frequency of Gus gene in Alondra's with the concentration of Agrobacterium tumefaciens bacterial to OD600=0.5 and the co-culture to 2 days. This work would provide reference for the genetic transformation in wheat.
【Fund】: 国家转基因重大专项(2008ZX08002-001);; 国家863项目(2006AA10Z1F6);; 高等学校学科创新引智计划111项目(B08025)共同资助
【CateGory Index】: S512.1
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