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《Molecular Plant Breeding》 2010-03
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Elimination of Potato Virus X and Potato Spindle Tuber Viroid by Cryopreservation

Bai Jianming 1,2,3 Chen Xiaoling 2 Lu Xinxiong 2 Guo Huachun 1 Xin Xia 2 Zhang Zhie 2 1 College of Agricultural Sciences and Biotechnology,Yunnan Agricultural University,Kunming,650201;2 Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing,100081;3 Institute of Economic Crops,Yunnan Academy of Agricultural Sciences,Kunming,650205  
Virus disease severely impair for potato production,are the major factor of potato yield decrease and the quality decline. Potato virus X(PVX) and potato spindle tuber viroid(PSTVd) are the main viruses damaging potato. In this study,we tried eliminating PVX and PSTVd from test-tube plantlet of potato by using cryopreservation and conventional method,such as tip meristem culture,thermotherapy,and thermotherapy followed by tip meristem culture. The results showed that the survival rate and regeneration rate of potato shoot tips were 83.64% and 72.04%,respectively by cryopreservation,all higher than tip meristem culture(46.67 % and 31.49%) ,thermotherapy(72.22% and 44.44%) and thermotherapy followed by meristem culture(50.54% and 30.38%) . PVX could be removed by four methods. The highest elimination rate of 59.13% by cryopreservation method. The elimination rates of thermotherapy followed by tip meristem culture was 56.02%,and that of thermotherapy and tip meristem culture were lower,42.61% and 35.83% respectively. But PSTVd couldn't be effectively eliminated by four methods and could be controlled by strict quarantine.
【Fund】: 国家科技支撑计划课题(2006BAD13B10);; 中央级公益性科研院所基本科研业务费专项资助项目(092060302-07);; 农业部农作物种质资源保护利用专项资助项目共同资助
【CateGory Index】: S435.32
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