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《Molecular Plant Breeding》 2013-05
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Cloning and Preliminary Functional Analysis of sx Gene in Petunia hybrida

Zhu Jianbo;He Li Qiu;Honglin Cheng;Quan Yan;Tiantian Cheng Chen;Key Laboratory of Agricultural Biotechnology of Shihezi University;  
A 417 b p fragm ent was c loned from Petunia hybrida cDNA by using some internet datebases and biological softwares such as NCBI, CODEHOPE, Primer Premier 5.0 to design degenerate primers and degenerate primers RT-PCR. Through analysis in GenBank. It has the highest similarity with SX gene sequence. Designing a specific primer according to the sequence of SX gene, finally a 747 bp fragment was obtained by RT-PCR. The result showed that SX was 660 bp in size and encoded 220 amino acids, including a transmembrance signal domain of 22 amino acids, the conserved regions(C1-C5) and hyper-variable regions(HVa and HVb). And then a signal peptide of 22 amino acids was removed to make it has a function of S-RNase in pollen, the pollen-specific LAT52 promoter of tomato was used to express sx, and a plant expression vector was constructed. Transgenic Arabidopsis plants expressing sx can influence the development of Arabidopsis' s floral organ, the pollen vitality is very low and even no vitality, and cause the infertility of transgenic as a result.
【Fund】: 兵团博士基金(2009jc01)资助
【CateGory Index】: S681.6
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