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《Molecular Plant Breeding》 2017-02
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Development and Application of Rice Blast Resistant Gene Pita Genespecific Molecular Marker

Cai Haiya;Zhou Lei;Jiao Chunhai;Fu Youzhi;You Aiqing;Xu Deze;Institute of Food Crops,Hubei Academy of Agricultural Sciences,Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement;  
Pita is a rice blast resistant gene with broad-spectrum. The coding sequence of the Pita allele was amplified by PCR and the products were sequenced and alimented and a specific sequence(GCC for resistant allele and CTAT for susceptible allele) at +2 001 bp in coding sequence in intron was identified. A set of codominant marker, used for identifying the target locus and then to decide the type of the Pita allele, was developed based on PCR-CTPP( PCR with confronting two-pair primers) method. The results showed that our marker have the advantage of high specificity, pretty stability, accuracy, simplicity, speediness and low cost. So it can be put to a good use in molecular marker-assisted selection breeding or Pita genotype discriminate in rice germplasm.
【Fund】: “七大农作物育种”试点专项(2016YFD0102103);; 科技部国际合作专项(2011DFB31620);; 国家973计划课题(2013CBA01405);; 湖北省农业科学院青年科学基金项目(2015NKYJJ02)共同资助
【CateGory Index】: S435.111.41
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Liu Wuge 1 Wang Feng 1* Liu Zhenrong 1 Zhu Xiaoyuan 2 Li Jinhua 1 Huang Huijun 1 Liao Yilong 1 Zhu Manshan 1 Fu Chongyun 1 Chen Jianwei 1 1 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640; 2 Plant Protection Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640;Improvement of Rice Blast Resistance in CMS Line Rongfeng A by Pyramiding Pi-1 and Pi-2 with Molecular Marker Techniques[J];分子植物育种;2012-05
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Cai Haiya;Zhou Lei;Jiao Chunhai;Fu Youzhi;You Aiqing;Xu Deze;Institute of Food Crops,Hubei Academy of Agricultural Sciences,Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement;;Development and Application of Rice Blast Resistant Gene Pita Genespecific Molecular Marker[J];分子植物育种;2017-02
2 Gong Shaolong;Hou Maolin;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;;Research progress on rice varietal resistance to the brown planthopper and white-backed planthopper[J];植物保护;2017-01
3 YAN Xiao-wei;TANG Qing-jie;HAN Yi-sheng;WANG Hui-jian;XING Fu-neng;TANG Li-qiong;XU Jing;Cereal Crops Research Institute,Hainan Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding of Hainan Province;;Breeding and Application of Restorer Line Haihui 818 with Multiple Resistance Genes in Rice[J];杂交水稻;2017-01
4 SHENG Wentao;CHAI Xuewen;RAO Yousheng;Department of Biological Technology,Nanchang Normal University;;Advances on Insect-resistant Breeding Utilized with Wild Rice Germplasm Resources in Rice[J];华北农学报;2016-S1
5 XIA Ming-yuan;LI Jin-bo;WAN Bing-liang;CHANG Hua-min;ZHAO Kai-rong;ZHA Zhong-ping;DU Xue-shu;YIN Desuo;QI Hua-xiong;Food Crops Research Institute,Hubei Academy of Agricultural Science;Zhongken Jinxiu Huanong Wuhan Science and Technology Co.,Ltd.;Yichang Academy of Agricultural Science Research;;Breeding and Application of a New Elite Indica Middle Three-line Hybrid Rice Combination,Ju-2-You 108[J];湖北农业科学;2016-23
6 YIN De-suo;LI Jin-bo;WAN Bin-liang;FANG Guo-cheng;QI Hua-xiong;Food Crop Research Institute,Hubei Academy of Agricultural Science,Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement;;Study on Improving Grain Number of Japonica Rice by Using Gn1a Gene[J];湖北农业科学;2016-21
7 Zhou Lei;Cai Haiya;Dai Fengmei;Xu Huashan;Liu Kai;Zha Wenjun;Li Sanhe;Yang Guocai;Chen Zhijun;Li Peide;Fang Guocheng;Jiao Chunhai;You Aiqing;Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Institute of Food Crops,Hubei Academy of Agricultural Sciences;Graduate School of Wuhan University;Hubei Collaborative Innovation Center for Grain Industry,Yangtze University;Hubei Academy of Agricultural Sciences;;Development and Application of a Functional SNP Marker of the Blast Resistant Gene Pi25 in Rice[J];分子植物育种;2016-10
8 Lin Kaiqin;Zhao Degang;Li Yan;He Xuanze;Wang Shaomin;Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region of Ministry of Education Guizhou Collaborative Innovation Center for Mountain Ecology and Agro-Bioengineering College of Life Sciences/Institute of Agro-Bioengineering,Guizhou University;Fenghuangshan National Forest Park;;Development of Gender-Related EST-SSR Markers in Eucommia ulmoides[J];林业科学;2016-10
9 WANG Hui;YAN Zhi;CHEN Jin-jie;ZHOU Gui-xiang;FANG Yu;WANG Lin;HUANG Yan-ling;ZHANG Cong-he;ZHANG Yun-hu;CHEN Lin;Anhui Win-all Hi-tech Seed Co.,Ltd./Key Laboratory for New Variety Development of Hybrid Rice,Ministry of Agriculture;;Research Progress and Prospect of Brown Planthopper Resistance Genes in Rice[J];杂交水稻;2016-04
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7 CHEN Hong-qi1, CHEN Zong-xiang2, NI Shen1, ZUO Shi-min2, PAN Xue-biao2,, ZHU Xu-dong1,(1State Key Laboratory of Rice Biology,China National Rice Research Institute, Hangzhou 310006, China;2Key Laboratory of Plant Functional Genomics,Ministry of Education;Yangzhou University,Yangzhou 225009,China);Pyramiding Three Genes with Resistance to Blast by Marker-Assisted Selection to Improve Rice Blast Resistance of Jin 23B[J];中国水稻科学;2008-01
8 JIN Su-juan1,2,#,LIU Wu-ge1,#,ZHU Xiao-yuan3,WANG Feng1,,LI Jin-hua1,LIU Zhen-rong1,LIAO Yi-long1,ZHU Man-shan1,HUANG Hui-jun1,LIU Yi-bai2(1Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;2 Agronomy College,Jiangxi Agricultural University,Nanchang 330045,China;3Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;# These authors contributed equally to this paper);Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Line GD-8S by Molecular Marker-Assisted Selection[J];中国水稻科学;2007-06
9 DENG Qi-ming 1,2,3, WANG Shi-quan 1,2,3, ZHENG Ai-ping 1,2,3, ZHANG Hong-yu 1,3, LI Ping 1,2,3,*( 1Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2Sichuan Provincial Center for Agri-Biotech Research, Chengdu 611130, China; 3Key Laboratory of Southwest Crop Genetic Resource and Improvement, Ministry of Education, Sichuan University, Ya’an 625014, China; );Breeding Restorer Lines with High Resistance to Bacterial Blight in Hybrid Rice by Using Molecular Marker-Assisted Selection[J];中国水稻科学;2006-02
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