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《Guizhou Agricultural Sciences》 2006-01
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Study on Cultivation Techniques for Virus-free Seedling of Sugarcane

ZHOU Min-qiang,YI Dai-yong,BAN Xiu-wen,GONG De-yong,LIU Fan-zhi(Guizhou Institute of Subtropical Crops,Zhenfeng 562200,China)  
The culture techniques including treatments of sugarcane buds by hot water,induction culture of sugarcane stem apex,selection of culture medium,effects of survival rate of seedling on size of stem apex,multiplicative culture of virus-free seedling,measures to control contamination from multi-phenol oxidation training and transplanting of virus-free seedlings and virus-free effects of virus-free seedling are studied.The results show that the hot water treatment and tissue culture can eliminate sugarcane virus effectively,the different medium have the different effects on meristematic tissue of sugarcane stem apex,the different growth hormones reveal different effects on survival rate of seedlings cutured from sugarcane stem apex.The better culture results can be obtained when stem apexes with 1~2 cm and 1~2 leaf primordium are cultured on No.I medium(MS+2.5 mg/L BA+0.2 mg/L NAA+0.5 g/L AC+ 2.5% sucrose).The contamination from multi-phenol oxidation can be effectively controlled when the medium is added with 0.5 g/L AC.
【Fund】: 贵州省农业厅项目(2002-2005)
【CateGory Index】: S566.1
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