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《Guizhou Agricultural Sciences》 2008-06
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Extraction of Total RNA of Potato Seeding in Vitro and Its Quality Analysis

LI Fei1,HE Yun-kun2,ZHANG Min1,LIU Jie3(1.Guizhou Potato Institute,Guiyang,Guizhou 550006;2.Guizhou Institute of Science and Technology Information,Guiyang,Guizhou 550004;3.Institute of Vegetable and Flower,China Academy of Agricultural Sciences,Beijing 100081,China)  
The total RNA extracted from seedlings in vitro of potato line H154 with good dry resistance by Trizol method was analyzed by gel electrophoresis.The results showed that some clear spectral bands were detected,the brightness ratio between 28S and 18S band was near 2∶1,the average value of OD_260/OD_280 was 1.91 and its concentration average value was 4.65 g/L The extracted Total RNA with good completeness,high purity and high content can provide the basis for studying cloning and analysis of related genes with dry resistance in potato to a certain degree.
【Fund】: 贵州省农业动植物育种专项“马铃薯抗旱育种及分子标记应用”[黔农育专字(2006)030]
【CateGory Index】: S532
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