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Diagnosis of Highly Virulent MDV Infected Immunized Chicken Flocks

WU Song-cheng1,SHI Kai-zhi2,3,LUO Ming-xing3,WANG De-sheng3,LI Yong-ming3(1.Guizhou Institute of Animal Husbandry and Veterinary,Guiyang,Guizhou 550005;2.Husbandry and Veterinary Institute of Bijie,Bijie,Guizhou 550017;3.Institute of Animal Disease,Guizhou University,Guiyang,Guizhou 550025,China)  
In order to make confirmed diagnosis of the infected chickens,the samples were examined through epidemiological and serological investigation and histological examination.And 132 bpr gene of MDV-1 as specific primer was synthesized for PCR amplification.The results indicated that the immunized chicken flocks were infected by highly virulent MDV.
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