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《Guizhou Agricultural Sciences》 2011-07
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Variation Analysis of Partial Sequences on Growth Hormone Receptor(GHR) Exon 10 in Ningqiang Pony and Kazakh Horse

WANG Jin,ZHANG Xiao-kang,ZHANG Cui-cui,LIU Fang-ni,CHEN Jiang-wei,ZHANG Tao,LU Hong-chao(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000,China)  
The sequences of grown hormone receptor(GHR) exon 10 of Ningqiang pony,Kazakh horse,and other animals were studied by PCR amplification,gene sequencing of Ningqiang pony and Kazakh horse,software DNAMAN was used for comparative analyze of the sequences of GHR exon10 in different animals,and consequently constructed the phylogenic tree based on GHR gene exon 10 sequence in different animals to provide the scientific basis in molecular level for the study on molecular systematic status and growth mechanisms of Ningqiang pony and Kazakh horse.The results showed that there was one SNP locus in the coding region.There was a base deletion A in 539 locus of Ningqiang pony and a transition of A to G in Kazakh horse in this locus compared with the standard sequence of AF39287 in horse,these variation caused the changes of amino acid,which might affect its biological function.The construction of phylogenic tree showed that the genetic relationship of studied animals was identical with the biological cladogram in terms of the sequence of GHR.
【Fund】: 陕西省教育厅项目“青年科研基金”(07JK212);; 陕西理工学院项目“大学生创新实验”(UIRP1040)
【CateGory Index】: S821.2
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