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《Guizhou Agricultural Sciences》 2012-11
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Tissue Culture Medium Optimization and Active Ingredient Analysis of Liquorice Tissue Culture Plantlets

LIANG Yuyong1,LI Xuejiao2,YIN Shuangshuang2,GAO Wenyuan2,ZUO Beimei3(1.Tongren Polytechnic,Tongren,Guizhou 554300;2.Tianjin University,Tianjin 300000;3.Tianjin University of Science and Technology,Tianjin 300457,China)  
In order to explore the optimization conditions for tissue culture and breeding of different parts of sterile liquorice seedlings and their active ingredient contents,the suitable explants,optimum culture medium of tissue culture seedling and active ingredients of different parts of liquorice were studied by using tissue culture technique,HPLC and ultraviolet spectrophotometry.Results: 1) the suitable explant for tillering culture was 4~7 day-old sterile cotyledon segments and the culture medium was MS+0.2 mg/L NAA+1.0 mg/L 6-BA.2) the seedling rate of euphylla segment reached 79.33% on the culture medium MS+3.0 mg/L IBA +0.1 mg/L KT,but the rooting effect of stem tip was the best on the same culture medium,the seedling rate was up to 94.67%.3) the transplanting survival rate of 30 day-age plantlets was over 88% when taking vermiculite and river sand as the substrates.4) the contents of the eight kinds of licorice flavonoids were all lower than that in liquorice medicinal material,but the total flavonoid content was higher than liquorice medicinal material;the polysaccharide content in liquorice tissue culture plantlet was 154.2 mg/g,significantly higher than that in liquorice medicinal material(145.5 mg/g).Various parts of liquorice,culture medium,transplanting time and substrate could all significantly influence the seedling rate and transplanting survival rate.All the liquorice tissue culture plantlets contained liquorice flavonoids and liquorice polysaccharide.
【Fund】: 国家教育部青年骨干教师访学基金资助项目“天然药物研究”[武汉中心函(2011)4]
【CateGory Index】: S567.71
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