Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Guizhou Agricultural Sciences》 2012-11
Add to Favorite Get Latest Update

Tissue Culture Medium Optimization and Active Ingredient Analysis of Liquorice Tissue Culture Plantlets

LIANG Yuyong1,LI Xuejiao2,YIN Shuangshuang2,GAO Wenyuan2,ZUO Beimei3(1.Tongren Polytechnic,Tongren,Guizhou 554300;2.Tianjin University,Tianjin 300000;3.Tianjin University of Science and Technology,Tianjin 300457,China)  
In order to explore the optimization conditions for tissue culture and breeding of different parts of sterile liquorice seedlings and their active ingredient contents,the suitable explants,optimum culture medium of tissue culture seedling and active ingredients of different parts of liquorice were studied by using tissue culture technique,HPLC and ultraviolet spectrophotometry.Results: 1) the suitable explant for tillering culture was 4~7 day-old sterile cotyledon segments and the culture medium was MS+0.2 mg/L NAA+1.0 mg/L 6-BA.2) the seedling rate of euphylla segment reached 79.33% on the culture medium MS+3.0 mg/L IBA +0.1 mg/L KT,but the rooting effect of stem tip was the best on the same culture medium,the seedling rate was up to 94.67%.3) the transplanting survival rate of 30 day-age plantlets was over 88% when taking vermiculite and river sand as the substrates.4) the contents of the eight kinds of licorice flavonoids were all lower than that in liquorice medicinal material,but the total flavonoid content was higher than liquorice medicinal material;the polysaccharide content in liquorice tissue culture plantlet was 154.2 mg/g,significantly higher than that in liquorice medicinal material(145.5 mg/g).Various parts of liquorice,culture medium,transplanting time and substrate could all significantly influence the seedling rate and transplanting survival rate.All the liquorice tissue culture plantlets contained liquorice flavonoids and liquorice polysaccharide.
【Fund】: 国家教育部青年骨干教师访学基金资助项目“天然药物研究”[武汉中心函(2011)4]
【CateGory Index】: S567.71
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 GE Shu-jun1,LI Guang-min1,MA Zhi-ying1,WANG Wen-quan2,WU Xiao-yang1,YUAN Jing-ya1,MENG Yi-jiang1(1.Agricultural University of Hebei,Baoding 071001,China;2.Beijing University of Chinese Medicine,Beijing 100102,China);Establishment of micropropagation system of Glycyrrhiza uralensis[J];Acta Prataculturae Sinica;2007-06
2 JI Chen-feng, JIANG Wei,WANG Xiao-jing (Postdoctal Programme of Institute of Materia Medica ,Harbin University of Commerce, Harbin 150076, China);Study on chemistry and pharmacology of glycyrrhiza polysaccharide[J];Journal of Harbin University of Commerce(Sciences Edition);2004-05
3 XING Guo-xiu, LI Nan, WANG Tong, YAO Mei-yan (1. College of Chemical Engineer Dalian University of Technology, Dalian 116012, Liaoning, China; 2. Shengyang Pharmaceutical University, Shengyang 110016, Liaoning, China);Advances in Studies on Flavonoids of Licorice[J];China Journal of Chinese Materia Medica;2003-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Xiao-feng et al(College of Life Science and Engineering,Lanzhou University of Technology,Lanzhou,Gansu 730050);Study on Extraction Process of Glycyrrhiza Flavonoids[J];Journal of Anhui Agricultural Sciences;2009-06
2 ZHANG Rui,LIN Qiang(Biochemical Engineering College of Beijing Union University,Beijing 100023,China);A Study on Separating Licorice Flavonoids by HP-20 Macroporous Resin[J];Journal of Beijing Union University(Natural Sciences);2006-02
3 WANG Li, ZHANG Baoxu, YAN Lei,JIA Guang,ZHANG Jingyi (College of Public Health,Inner Mongolia University of Science and Technology,Baotou 014010,China; Department of Medicine,Beijing University,Beijing 100083,China);Influence of Curcumin,Glyinflanins and Chalcone on Biological actions of Human MGC-803[J];Journal of Baotou Medical College;2005-01
4 DONG Ran1,PAN Yan-yan1,WANG Li-qing2,GU De-feng1,LIU Hong-zhang1(1.Jilin Agricultural University,College of Horticulture,Changchun 130118,China;2.Panshi Horticultural Specialty Technical Popularization Station of Jilin Province,Panshi 132300,China);Establishment of micropropagation system of Ligularia jaluensis Kom.[J];Acta Agrestia Sinica;2010-02
5 ZHAO Jie,ZHANG Bo,WANG Yu-xiang,JIA Guang-yun,Li Pei-ying (Key Laboratory of Grassland Resource and Ecology of Xinjiang,College of Grassland Science and Environmental,Xinjiang Agricultural University,Urumqi 830052,China);Study on in vitro propagation of Alhagi sparsifolia cuttings[J];Pratacultural Science;2009-05
6 YUAN Xue-jun1,2,WANG Zhi-yong1,GUO Ai-gui1,ZONG Jun-qin1,LIU Jian-xiu1,SHE Jian-ming1 (1.Grass Research Centre,Institute of Botany,Jiangsu Province & Chinese Academy of Sciences,Nanjing 210014,China;2.College of Life Science,Nanjing Agricultural University,Nanjing 210095,China);Callus induction and plantlet regeneration from axillary buds of Eremochloa ophiuroides[J];Acta Prataculturae Sinica;2008-06
7 XU Chun-bo1,MI Fu-gui2,WANG Yong2,LI Xing-you1,ZHAO Hai-xia1(1.Grassland Research Institute of Chinese Academy of Agricultural Sciences,Huhhot 010010,China;2.College of Ecology and Environment,Inner Mongolia Agricultural University,Huhhot 010018,China);Factors influencing plant regeneration systems in mature embryo culture of Agropyron[J];Acta Prataculturae Sinica;2009-01
8 GUO Ye-hong,LIN Hai-ming,WU Rui(Agriculture college,Gansu Agricultural University,Lanzhou 730070,China);Research on tissue culture and medium of Nitraria tangutorum[J];Acta Prataculturae Sinica;2009-06
9 YIN Ying-chun,SHI Gui-ying,LI Jiang,XU Xue-qin(Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety,and Department of Chemistry,Fuzhou University,Fuzhou 350108,China);Electrochemical Behavior of Liquiritin at Glassy Carbon Electrode and Its Determination[J];Analysis and Testing Technology and Instruments;2012-02
10 YOU Wei,ZHENG Zhi-xiang and GAO Zuo-ning*(Key Lab of Energy Source and Chemical Engineering,College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021);Electrochemical behaviors of liquiritin at hanging mercury drop electrode and its electrochemical determination[J];Chinese Journal of Analysis Laboratory;2008-07
China Proceedings of conference Full-text Database 4 Hits
1 SUN Chen~ *,ZHANG Haiming (Chemistry and Chemical Engineering College of Henan University of Technology,Zhengzhou 450001);Study on Aqueous Two-phase Extraction of Potassium Glycyrrhizanate by Polyethylene Glycol/(NH_4)_2SO_4 System[A];[C];2011
2 SUN Jiaming1,ZHANG Hui (Development Center of Traditional Chinese Medicine and Bioengineering,Changchun University of Traditional Chinese Medicine,Changchun 130117,China);Analysis of Components in the Aqueous of Glycyrrhiza Uralensis Fisch.and Glycyrrhiza Uralensis Fisch.Preparated with Honey by SPE-HPLC-ESI/MS/MS[A];[C];2009
3 ZHAO Jun-Xia~2,YUAN Guang-Feng~1,ZHANG Shu-Bin~1,WANG Li-An~1 (1.The Life Science College,Hebei Normal University,Shijiazhuang,050016;2.The Basic Medical College, Hebei Medical University,Shijiazhuang,050017,China);The Analysis of Crude Triterpenoids and Flavonoids Contents,Antioxidative and Antitumor Activities of the Crude Extracts from Culture Broth of Volvariella volvacea[A];[C];2007
4 Jiaming SUN,Hui ZHANG (Development Center of Traditional Chinese Medicine and Bioengineering,Changchun University of Traditional Chinese Medicine,Changchun,130117,China);Analysis of Components in the Aqueous of Glycyrrhiza uralensis Fisch.and Glycyrrhiza uralensis Fisch. Preparated with honey bv SPE-HPLC-ESI/MS/MS[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 RUAN Ming et al(Institute of Medicinal Fungi,Department of Life Science of Nanjing Xiaozhuang University,Nanjing,Jiangsu 211171);Content Determination of Polysaccharide in Zhi Qi Fungal Substance by Anthrone-sulfate Method[J];Journal of Anhui Agricultural Sciences;2008-27
2 JIANG Shu-hui,GUAN Rong-zhan,DONG Hai-bin,DU Wen-ming(National Key Lab of Crop Genetics and Germpalsm Enhancement,Nanjing Agricultural University,Nanjing 210095,China);Studies on protoplast culture of Descurainia sophia calli[J];Acta Prataculturae Sinica;2006-04
3 LIU Qing-fang,BU Huai-yu,ZHAO Yu-wei,JIA Jing-fen(Life Science School,Northwest University,Xi'an 710069,China);Plant regeneration in vitro and RAPD analysis of Medicago sativa[J];Acta Prataculturae Sinica;2006-05
4 Zhang Xinling ( Agricultural College,Xinjiang Agricultural University ,Urumqi 830052)Li Xueyu,Wei Lingji and Cui Jianping ( Agricultural College,Shihezi University ,Shihezi Xinjiang 832003);THE INTERSPECIFIC HYBRIDIZATION OF GLYCYRRHIZA IN XINJIANG[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1998-01
5 Du Xuemei and Li Dengke (Institute of Pomology, Shanxi A cademy of Agricultural Sciences, Taigu, Shanxi 030800 China);Advances in Research of Biotechnology Breeding for Deciduous Fruit Crops[J];Journal of Fruit Science;2002-05
6 LI Shan-jia,WANG Yu-li,YANG Ming-jun,LIU Zuo-jun,WANG Ming-gang(School of Life Science and Engineering,Lanzhou University of Science and Technology,Lanzhou 730050,China);Study on the New Techniques for Extracting Glycyrrhizic Acid from G. uralensis Fisch[J];Journal of Gansu Sciences;2010-02
7 LI Run-feng,CHANG Xue-dong,DI Xiao-li(College of Food Engineering,Hebei Normal University of Science & Technology,Qinhuangdao Hebei,066600,China);Determination of Chestnut Polysaccharide Content with Anthrone-sulfuric Acid Method[J];Journal of Hebei Normal University of Science & Technology;2010-02
8 WANG Hang yu, LIU Jing rong, JIANG Fa shou et al. (College of Pharmacy, Shihezi University, Shihezi 832002, China);Ultrasonic Extraction and Determination of Polysaccharide of Glycyrrhiza inflata Bat.[J];Primary Journal of Chinese Materia Medica;2002-01
9 Wang Chen, Shi Yurong, Xie Guangru, et al. Tianjin Cancer Institute, Tianjin 300060;Study on the anti-tumor effect in vivo of Glycyrrhizia polyoaccharide and its mechanism[J];Chinese Clinical Oncology;2003-02
10 ZHANG Hong-xiao, JING Jian-ying, ZHANG Guan-chen (CoUege of Forest-profession, Henan University of Science and Technology, Luoyang 471002, China; Department of Environment and Chemistry, Luoyang University, Luoyang 471023, China);The Appliance of Tissue Culture in Plant Breeding[J];Journal of Luoyang University;2003-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved