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Isolation and Antimicrobial Activity of Endophytic Fungi in Coix lacryma-jobi Kernels

ZHANG Wanqin;WEN Tingchi;QIU Guojun;College of Biology&Chemistry,Xingyi Normal University for Nationalities;College of Life Sciences,Guizhou University;  
The antimicrobial activity of endophytic fungi isolated fromCoix lacryma-jobi kernels was screened by the colony method,then the strains were identified by 5.8SITS molecular systematics,and finally secondary metabolites extracted from strain fermentation liquor were analyzed by HPLC.The results showed that 23 endophytic fungi strains including Curvularia, Alternaria, Dictyomitosporicfungi,Phoma sp.,Mollisia cinerea,Nigrospora oryzae,Gibberella zeae,Aspergillus and other fungi are isolated.57% strains has the antimicrobial activity against one test strain at least.GZR19 strain with broad-spectrum antimicrobial activity is identified as Phoma sp.and there are main four substances in secondary metabolites of the fermentation liquor,which indicates that the compounds with antimicrobial activity may exist in four substances.
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