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《Guizhou Agricultural Sciences》 2014-10
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Isolation and Antimicrobial Activity of Endophytic Fungi in Coix lacryma-jobi Kernels

ZHANG Wanqin;WEN Tingchi;QIU Guojun;College of Biology&Chemistry,Xingyi Normal University for Nationalities;College of Life Sciences,Guizhou University;  
The antimicrobial activity of endophytic fungi isolated fromCoix lacryma-jobi kernels was screened by the colony method,then the strains were identified by 5.8SITS molecular systematics,and finally secondary metabolites extracted from strain fermentation liquor were analyzed by HPLC.The results showed that 23 endophytic fungi strains including Curvularia, Alternaria, Dictyomitosporicfungi,Phoma sp.,Mollisia cinerea,Nigrospora oryzae,Gibberella zeae,Aspergillus and other fungi are isolated.57% strains has the antimicrobial activity against one test strain at least.GZR19 strain with broad-spectrum antimicrobial activity is identified as Phoma sp.and there are main four substances in secondary metabolites of the fermentation liquor,which indicates that the compounds with antimicrobial activity may exist in four substances.
【Fund】: 贵州省教育厅自然科学研究重点项目“薏苡内生真菌的分离及其次生代谢产物活性研究”[黔教合KY字(2013)190];; 贵州省普通高等学校创新人才团队“民族药用生物资源研究与开发创新团队”[黔教合人才团队字(2013)30]
【CateGory Index】: S519;Q93
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