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《Guizhou Agricultural Sciences》 2015-05
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Structure of Plant Cell Wall Degrading Enzymes from Fusarium Strain Q7-31T

ZHAO Lianzheng;XIE Zhanling;JIA Xianqing;College of Eco-Environmental Engineering,Qinghai University;National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm;  
For exploring the mechanism of high-efficiency plant cell wall degradation process based on plant cell wall degrading enzymes from Fusarium strain Q7-31 T,for the gene cloning and biological analysis,the methods respectively were PCR amplification and some bioinformatic softwares such as ProtParam,SOPMA and ProtScale Server.Results:The plant cell wall degrading enzymes in Q7-31 Tare classified as:GH5family endoglucanase,GH7 family endoglucanase,GH7 family exo-glucanase and GH10 family endoxylanase,which are consistent with mass spectrometry.The enzymes are medium molecular size,and the secondary structure consists of four elements:α-helix,extended strand,β-turn and random coil,of which random coil is most abundant;four enzyme classes are hydrophilic enzymes,and have higher proportion of phosphorylation sites.There are 1~2glycosylation sites.Four enzymes are present in a higher proportion of catalytic domains and the tertiary structure of a hollow "C"shape.Conclusion:A small number of glycosylation sites,high proportion of catalytic domains,changeable threedimensional conformations,large number of phosphorylation sites and high-efficiency synergism probably determine the high-efficiency degradation of plant cell wall degrading enzymes in strain Q7-31 T.
【Fund】: 国家自然科学基金资助项目“Q7-31植物细胞壁降解酶系组成及协同作用研究”(31260021)
【CateGory Index】: Q93
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