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Determination of Sugar Content by DNS Colorimetry in Tobacco

ZHOU Li jun ZHOU Shu ping HAN Feng(Central Laboratory,Guizhou Academy of Agricultural Sciences,Guiyang 550006)  
DNS colorimetry(3,5 dinitrosalicylic acid)was used to replace the way of classical KMnO 4 to determine sugar contents in tobacco.According to our repeated experiments it is known that brown reactant from 3,5 dinitrosalicylic acid reducing sugar of tobacco samples could keep stable for 6 hours after reaction,and recollection rate is 100 2%.And the accuracy is higher and cost lower compared with KMnO 4 titration way This way especially suitable for determining large quantity of samples.
【CateGory Index】: S572
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