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Formation and Regeneration of Protoplasts from Paecilomyces Cicadae

ZHANG Yong jun LIU Ai Ying LIANG Zong qi (Laboratory of Entomogenus Mycology, Guizhou University,Guiyang 550025 CHINA)  
The effects of such factors as enzyme systems, media for mycelia growth, osmotic stabilizers, different temperatures and age of mycelium on the formation of the mycelia protoplasts were studied.High yield of protoplasts were obtained from 16 hours mycelia of Paecilomyces cicadae by using 1.5% lyvallzyme as enzyme system, starch agar medium as mycelia; growth medium and 0.6% KCl as osmotic stabilizer at the temperature of 28℃. When 0.6M NaCl was used as stabilizer, about 7.97% of protoplasts regenerated and formed colonies on soybean medium (SOS). The morphological processes of protoplasts and regeneration were illustrated by microphotograph.
【Fund】: 贵州省基金
【CateGory Index】: Q939.5,
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