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《Journal of Clinical Hepatology》 2012-03
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Distribution and expression of adenovirus vector in mice with MHV-3-induced fulminant hepatitis

Wang Ming,Guo Jianwen,Xi Dong,et al.Institute of Infectious Disease,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China  
Objective To study the distribution and expression of β-galactosidase(LacZ) reporter gene mediated by adenovirus vector in the liver of normal mice or mice with fulminant hepatitis,so as to evaluate the efficacy and safety of adenovirus as a gene therapy vector transduction system.Methods The normal Balb/cJ mice were randomly assigned to normal and fulminant hepatitis group.Each mouse was injected with adenovirus at 2×108PFU by tail vein injection,then the normal mice serum was collected to detect ALT and TBil at 24h,48h,72h,96h,120h,and the seventh day,meanwhile,the mice organs including liver,lung,heart,and kidney were collected and stained by X-gal in order to observe the expression of adenovirus vector in mice organ;the expression of adenovirus vector in liver of fulminant hepatitis mice induced by MHV-3 was detected.Results Adenovirus mainly expressed in the livers of mice;In normal group and fulminant hepatitis group,the highest expression efficiency was at 72 hours,reached 55.7% and 25.7%,respectively,then it followed by a gradual reduction of adenovirus expression;The adenovirus infection didn't show obvious injury in liver function.Conclusions The adenovirus vector is highly expressed in the liver with no significantly liver damage;Adenovirus vector might be as an effective and safe gene delivery system for liver disease gene therapy.
【Fund】: 国家973项目(2007CB512900);; 国家自然科学基金项目(30700702);; “十一五”国家科技支撑计划项目(2006BAI05A07)
【CateGory Index】: R575
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