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《Journal of Clinical Hepatology》 2012-03
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Distribution and expression of adenovirus vector in mice with MHV-3-induced fulminant hepatitis

Wang Ming,Guo Jianwen,Xi Dong,et al.Institute of Infectious Disease,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China  
Objective To study the distribution and expression of β-galactosidase(LacZ) reporter gene mediated by adenovirus vector in the liver of normal mice or mice with fulminant hepatitis,so as to evaluate the efficacy and safety of adenovirus as a gene therapy vector transduction system.Methods The normal Balb/cJ mice were randomly assigned to normal and fulminant hepatitis group.Each mouse was injected with adenovirus at 2×108PFU by tail vein injection,then the normal mice serum was collected to detect ALT and TBil at 24h,48h,72h,96h,120h,and the seventh day,meanwhile,the mice organs including liver,lung,heart,and kidney were collected and stained by X-gal in order to observe the expression of adenovirus vector in mice organ;the expression of adenovirus vector in liver of fulminant hepatitis mice induced by MHV-3 was detected.Results Adenovirus mainly expressed in the livers of mice;In normal group and fulminant hepatitis group,the highest expression efficiency was at 72 hours,reached 55.7% and 25.7%,respectively,then it followed by a gradual reduction of adenovirus expression;The adenovirus infection didn't show obvious injury in liver function.Conclusions The adenovirus vector is highly expressed in the liver with no significantly liver damage;Adenovirus vector might be as an effective and safe gene delivery system for liver disease gene therapy.
【Fund】: 国家973项目(2007CB512900);; 国家自然科学基金项目(30700702);; “十一五”国家科技支撑计划项目(2006BAI05A07)
【CateGory Index】: R575
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Jian zheng,JIN Guang hui, TIAN Mei,PAN Xue na, JIN Shun zi,LIU Shu zheng  (MH Radiobiology Research Unit, School of Public Health,Jilin University,Changchun 130021,China);Cloning and sequencing of mouse B7-2 gene and construction of its recombinant plasmids[J];Journal of Jilin University of (Medicine Edition);2004-03
2 XU Ying-mei1, ZHANG Shu-biao1, HU Jian-jun2, LIU Dong-liang2, QIAO Wei-hong2, LI Zong-shi2 (1.Dept of Chemical Engineering Dalian Nationalities University, Dalian Liaoning 116600, China; 2.State Key Laboratory of Fine Chemicals Dalian Technology University, Dalian Liaoning 116012, China);Liposomes for Gene Delivery[J];Journal of Dalian Nationalities University;2004-05
3 CHEN Yan-xiang1,QIAO Wei-hong1,LIU Dong-liang2,LI Zong-shi1(1.State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian 116012,China;2.College of Chemistry and Chemical Engineering,Donghua University,Shanghai 201620,China);Targeted vectors of gene therapy[J];Journal of Dalian Medical University;2007-04
4 ;Expectation of gene therapy for epilepsy[J];Acta Academiae Medicinae Militaris Tertiae;2003-20
5 Zhong Yang,Li Jing,Huang Xiaobing,Zheng Lu,Yang Tonghan,Zhao Hongzhi,Liang Ping(Department of Hepatobiliary Surgery,Xinqiao Hospital,Third Military Medical University,Chongqing,400037,China);BC047440 silencing in HepG2 cells mediated by lentiviral vector[J];Acta Academiae Medicinae Militaris Tertiae;2010-08
6 LIU Li,WU De-hua,JIA Yu-rong.Department of Pathology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Effect and clinical significance of double suicide gene therapy on colorectal carcinoma cells[J];Guangdong Medical Journal;2006-06
7 GUO Yi, ZHANG Shuang-nan, KANG Chun-sheng, CHANG Jin*(Institute of Nanobiotechnology, School of material science and engineering, Tianjin University, Tianjin 300072,China);Application of Poly-cation Nanopaticles on Gene Delivery System[J];Polymer Bulletin;2005-05
8 LUAN Zhonghua, HAN Jinxiang, LU Yanqin Shandong Medical Biotechnology Center, Key Labora tory for Biotech-Drugs of Health, Ji' nan, 250062, China;Advance in the Research of Ribozymes Suppressing HBV[J];Journal of Medical Molecular Biology;2005-04
9 QI Li,GU Ming,CONG Wei(State Key Lab.Biochem.Eng.,Institute of Process Engineering,CAS,Beijing 100080,China);Progress in the Production of Adenovirus Vector[J];The Chinese Journal of Process Engineering;2004-05
10 ZHANG Wei,SHI Qing-hong(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China);Development of Starburst PAMAM Dendrimers as Gene Vector[J];Chemical Industry and Engineering;2009-02
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