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Studies on Malate Dehydrogenase Ⅱ(MDH Ⅱ) Isozymes in Different Races of Apis mellifera ligustica

Chen Shenglu,Bao Xiuliang,Su Songkun,Liu Yanhe (Research Institute of Apiculture,Zhejiang University,Hangzhou 310029,China)[  
The young workers (in three days) of five races of Apis mellifera ligustica ——ZAU-1 Italian honey bee (Ea),Chinese Italian honey bee (Eb),American Italian honey bee (Em),Italian Italian honey bee (Ee) and Australian Italian honey bee (Eo) have been used to study the genotype,genotype frequency,allele frequency,heterozygous and homzygous degrees of the malate dehydrogenase Ⅱ (MDH Ⅱ) isozymes with polyacrylamide gel isoelectrofocusing (IEF-PAGE).There are six genotypes (a/a,a/b,a/c,b/b,b/c,c/c) in MDH Ⅱ that is coded by three alleles:a ,b and c. All of them are found in Ea,Eb and Ee;four (a/b,a/c,b/c,c/c) are found in Eo; three (a/c,b/c,c/c) in Em The genotype frequencies are aa (0 022),ab (0 028),ac (0 296),bb (0 007),bc (0 140),cc (0 508) in Ea;aa (0 003),ab (0 011),ac (0 117),bb (0 006),bc (0 156),cc (0 706) in Eb;aa (0 033),ab (0 203),ac (0 224),bb (0 021),bc (0 252),cc (0 267) in Ee;aa (0 000),ab (0 000),ac (0 313),bb (0 000),bc (0 253),cc (0 433) in Em;aa (0 000),ab (0 006),ac (0 011),bb (0 000),bc (0 172),cc (0 811) in Eo.The allele frequencies are a (0 184),b(0 091),c(0 726) in Ea;a(0 067),b(0 090),c(0 717) in Em;a(0 008),b(0 089),c(0 903) in Eo.The heterozygosities (H) of the MDH Ⅱ locus equal to 0 464 in Ea;0 284 in Eb;0 679 in Ee;0 566 in Em;0 189 in Eo. The results of test independence show that there are significant differences in the distributions of genotype frequency,gene frequency,heterozygous and homozygous degrees among five races of Apis mellifera. [
【Fund】: 国家自然科学基金!(394 70 552 )
【CateGory Index】: S893.3
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【References】
Chinese Journal Full-text Database 3 Hits
1 ZENG Zhi-Jiang,XIE Xian-bing,YAN Wei-Yu,GUO Dong-sheng(College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China);Effects of Malate Dehydrogenase Ⅱof Worker Bees on Nutritional Crossbreed between Apis cerana Cerana and Apis mellifera Ligustica[J];Journal of Shanghai Jiaotong University(Agricultural Science);2006-01
2 XIE Xian-bing 1,3, PENG Wen-jun2, ZENG Zhi-jiang1 (1Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045; 2Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093; 3College of Quanzhou Normal, Quanzhou 362000, Fujian);Breeding of Mite-Resistant Honeybee by Using Nutritional Crossbreed Technology[J];Scientia Agricultura Sinica;2008-05
3 SUN Liang-xian , CHEN Chao-yang , YUANG Jian-jun, XIE Jin-jin (Model Organism Research Centre , Quanzhou Normal College, Quanzhou, 362000, China);Genetic Variability of MDHⅡin Four Lines of Apis mellifera ligustica[J];Journal of ZhangZhou Teachers College;2004-01
【Citations】
Chinese Journal Full-text Database 4 Hits
1 LI Juhuai (Beijing Academy of Agricultural Sciences)LI Shaowen (Department of Biology, Peking University);Electromorph and Genitype of Malate Dehydrogenase in Apis Mellifera[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1990-02
2 Li Juhuai(Crops Institute,Beijing Municipal Academy of Agricultural and Forestry Sciences,Beijing 100081 )Wang Xiaojing;Guan Yinghui;Li Shaowen(College of Life Sciences,Peking University, Beijing 100871)Chen Shenglu(College of Animal Sciences, Zhejiang Ag;Studies on MDH Isozyme among Subspecies of Apis Mellifera[J];ACTA AGRICULTURAE BOREALL—SINICA;1996-02
3 LI SHAO-WEN MENG YU-PIN J. T. CHANG(Department of Biology, Peking University)LI JU-HUAI (Beijing Academy of Agricultural Sciences);STUDIES ON FOUR ISOZYMES OF TWO SPECIES OF HONEYBEES, APIS MELLIFERA AND APIS CERANA[J];Acta Entomologica Sinica;1988-01
4 GUAN YING-HUI; LI SHAO-WEN(Department of Biology, Peking University , Beijing 100871)LI JU-HUAI (Beijing Academy of Agricultural and Forestry Sciences, Beijing 100081) CHEN SHENG-LU(Zhejiang Agricultural University, Hangzhou 310029);GENOVARIATION IN THREE RACES OF APISMELLIFERA LIGUSTICA[J];ACTA ENTOMOLOGICA SINICA;1994-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HOU LIN CAI HAN-JUN(Institute of Marine Resources, Liaoning Normal University, 116022)ZOU XIANG-YANG(Dalian Medical College, 116023);A STUDY ON ISOZYMES OF TEN ARTEMIA STRAINS FROM CHINA[J];Acta Zoologica Sinica;1993-01
2 TONG Fu Dan ** WANG Qiao Mei LIU Yan He (Zhejiang University,Hangzhou 310029);GENETIC DIFFERENCES IN PATTERNS OF EST ISOZYMES AND GENOTYPES OF MDHⅡ ISOZYMES IN FOUR SUBSPECIES OF APIS MELLIFERA[J];Acta Zoologica Sinica;2002-06
3 LI Juhuai (Beijing Academy of Agricultural Sciences)LI Shaowen (Department of Biology, Peking University);Electromorph and Genitype of Malate Dehydrogenase in Apis Mellifera[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1990-02
4 Li Juhuai(Crops Institute,Beijing Municipal Academy of Agricultural and Forestry Sciences,Beijing 100081 )Wang Xiaojing;Guan Yinghui;Li Shaowen(College of Life Sciences,Peking University, Beijing 100871)Chen Shenglu(College of Animal Sciences, Zhejiang Ag;Studies on MDH Isozyme among Subspecies of Apis Mellifera[J];ACTA AGRICULTURAE BOREALL—SINICA;1996-02
5 ZHANG Hong_jiegy, Shaanxi University of Technology,Hanzhong 723000, Shaanxi, China);Investigating Actuality of Insect Genetic Diversity in China[J];Journal of Hanzhong Teachers College;2003-03
6 LIU Yan He 1, CHEN Sheng Lu 1* , TONG Fu Dan 2, ZHANG Chuan Xi (1 Institute of Applied Entomology, Zhejiang University, Hangzhou\ 310029, China; 2 Institute of Biochemical Research, Zhejiang University, Hangzhou\ 310029, China);Genetic variability of MDHⅡgene in six subspecies of Apis mellifera[J];Acta Entomologica Sinica;2002-02
7 Tian Xuejun(Bee Research Institute, Yunnan Academy of Agricultural Siences,Caoba,Mengzi County,Yunnan Province,661101);Comparative studies on the morphology of Apis laboriosa Smith and Apis dorsata Fabr(Hymenoptera:Apidae)[J];JOURNAL OF BEE;1996-07
8 QI Baoying1,2, ZHENG Zheming2(1.Department of Biology,Inner Mongolia Normal University,Huhhot 010022,China;2.Institute of Zoology,College of Biological Science,Shaanxi Normal University,Xian 710062,China);<title>APPLICATION OF ISOZYME ELECTROPHORETIC TECHNIQUE AND DNA-RAPD TECHNIQUE IN ENTOMOLOGICAL RESEARCH[J];Journal of Inner Mongolia Normal University(Natural Science Edition);2002-03
9 Jiang Zhongxue Meng Xiangfeng(Dept.of Plant Protection, Henan Agricultural University, Zhengzhou 450002;Zhumadian Agricultural Research Institute,Zhumadian 463O00);Studies on the Isoenzymes of the Five Related Species of Ostrinia Insect Pests and Their Application in Classification[J];ACTA AGRICULTURAE UNIVERSITATIS HENANENSIS;1994-01
10 WANG Gui-zhi1,SHI Wei2* (1.College of Animal Science and Technology,Shandong Agricultural University,Tai'an 271018,China;2.The Institute of Bee,Chinese Academy of Agricultural Sciences,Beijing 100093,China);Pigmentation Diversity of Apis cerana Worker Bee Germplasm Resource in Middle and Lower Reaches of the Yellow River[J];Acta Agriculturae Universitatis Jiangxiensis;2009-05
China Proceedings of conference Full-text Database 1 Hits
1 DONG Jie, SUN Li-Ping, WANG Rui-WU (Institute of Apicultutal Research Chinese Academy of Agricultural Sciences , Beijing 100093, China);A study on mitochondrial DNA genetic diversity of Apis cerana ceranam different areas.[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YU Lin sheng 1,MENG Xiang jin 2 (1.Anhui Agricultural University,Hefei 230036;2.Anhui Agricultural Commission);Investigation on Postembryonic Growth of Apis mellifera L.[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-02
2 TAN Ken 1,Fiola BOCK2,Stefan FUCHS3,Sebastian STREIT2,Axel BROCKMANN4,Juergen TAUTZ 21.Eastern Bee Research Institute of Yunnan Agricultural University,Kunming 650201,China2.Beegroup Würzburg,Zoologie II,Biozentrum,Universitt,Am Hubland D-97074 Würzburg,Germany3.Institut für Bienenkunde(Polytechnische Gesellschaft),FB Biologie und Informatik der J.-W.Goethe Universitt Frankfurt an Main,Karl-von-Frisch-Weg 2,61440 Oberursel,Germany4.Department of Entomology,University of Illinois at Urbana-Champaign,320 Morill Hall,505 S.Goodwin Avenue,Urbana,IL 61801,USA;Effects of brood temperature on honey bee Apis mellifera wing morphology[J];Acta Zoologica Sinica;2005-04
3 Li Shaowen Meng Yupin J. T. Chang (Department of Biology, Peking University)Li Juhuai (Peking Academy of Agricultural Sciences)He Shaoyu Kuang Bangyu (Yunnan Agricultural University);Comparative Study of Esterase Isozymes in Six of Apis[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1986-04
4 LI Juhuai (Beijing Academy of Agricultural Sciences)LI Shaowen (Department of Biology, Peking University);Electromorph and Genitype of Malate Dehydrogenase in Apis Mellifera[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1990-02
5 Gong, Yi-fei (Dept.Head of Beekeeping,FAC);THE NATURAL BEEKEEPING CONDITIONS AND HONEY BEE RACES IN CHINA[J];;1983-03
6 ZHOU Bingfeng, LIN Shihuang, SU Jing, XUE Fenqin, JIANG Tianbao(College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Effects of temperature on the developments of honeybee oosperms and queen pupae[J];Journal of Fujian Agricultural University(Natural Science);2002-04
7 Li Juhuai(Crops Institute,Beijing Municipal Academy of Agricultural and Forestry Sciences,Beijing 100081 )Wang Xiaojing;Guan Yinghui;Li Shaowen(College of Life Sciences,Peking University, Beijing 100871)Chen Shenglu(College of Animal Sciences, Zhejiang Ag;Studies on MDH Isozyme among Subspecies of Apis Mellifera[J];ACTA AGRICULTURAE BOREALL—SINICA;1996-02
8 ZENG Zhi-Jiang1, YAN Wei-Yu1, XUE Yun-bo2, DING Neng-shui1 (1.College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China; 2. Jilin Apiculture Resources Institute,Jilin,Jilin 132108, China);Number of Drones Mated with Queen Determined Using RAPD Analysis[J];Journal of Economic Animal;2003-04
9 ZENG Zhi-jiang, YAN Wei-yu, PANG Hui-zhong, FAN Zhao-bin , XIE Guo-xiu (1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; 2. Department of Biotechnology, Jiangxi Agricultural University, Nanchang 330045, China );Study on the Physiological-Biochemistry Mechanism of Honeybee Sex Ratio Control[J];Journal of Economic Animal;2004-02
10 ZENG Zhi-jiang, YAN Wei-yu (College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045,China);Study on the Molecular Mechanism of Honeybee Sex Ratio Control[J];Journal of Economic Animal;2004-04
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 JI Cong-hui,DAI Rong-guo(Chongqing Academy of Animal Sciences,Chongqing,402460,China);Progress on Control Technique of Bee Mites[J];Progress in Veterinary Medicine;2009-10
2 WU Xiao-Bo,YAN Wei -Yu,ZENG Zhi-Jiang (Institute of Honeybee Research,Jiangxi Agricultural University,Nanchang 330045,China);Emergency cells and breeding broods of the introduced Apis species in queenless colonies of Apis mellifera ligustica and Apis cerana cerana[J];Chinese Bulletin of Entomology;2009-01
3 LIU Yi-bo,HUANG Qiang,YAN Wei-yu,XIE Xian-bing,ZENG Zhi-Jiang(Honeybee Research Institute of Jiangxi Agricultural University,Nanchang 330045,China);Study on the Selective Parasitism of the Varroa Mite to the Different Subfamilies by ISSR[J];Journal of Bee;2009-08
4 Wu Xiaobo Yan Weiyu Zeng Zhijiang (Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045);Study on Raising the Collaboration colonies of Apis cerana cerana and Apis mellifera ligustica and the Workers Policing in the Colonies[J];Apiculture of China;2008-01
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Li Shaowen Meng Yupin J. T. Chang (Department of Biology, Peking University)Li Juhuai (Peking Academy of Agricultural Sciences)He Shaoyu Kuang Bangyu (Yunnan Agricultural University);Comparative Study of Esterase Isozymes in Six of Apis[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1986-04
2 LI Juhuai (Beijing Academy of Agricultural Sciences)LI Shaowen (Department of Biology, Peking University);Electromorph and Genitype of Malate Dehydrogenase in Apis Mellifera[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1990-02
3 LI SHAO-WEN MENG YU-PIN J. T. CHANG (Department of Biology, Peking University)LI JU-HUAI (Peking Academy of Agricultural Sciences);STUDIES ON THE ISOZYMES OF HONEYBEES[J];Acta Entomologica Sinica;1985-04
4 LI SHAO-WEN MENG YU-PIN J. T. CHANG(Department of Biology, Peking University)LI JU-HUAI (Beijing Academy of Agricultural Sciences);STUDIES ON FOUR ISOZYMES OF TWO SPECIES OF HONEYBEES, APIS MELLIFERA AND APIS CERANA[J];Acta Entomologica Sinica;1988-01
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