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《Engineering Sciences》 2012-02
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A quick detection method for the early stage of disease in turbot (Scophthalmus maximus)——Development of the colloidal gold-based immunochromatographic strip

Wang Weifang1,Chai Shujun2,Liu Qingtang2,Lei Jilin1, Ding Fuhong1,Hong Lei1,Liu Xinfu1,Su Ke3(1.Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Henan Key Laboratory for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002, China;3.Qingdao General Aquaculture Co.,Ltd.,Qingdao 266404,China)  
In this study,an immunochromatographic strip was developed for the serological detection of antibodies against bovine serum albumin(BSA) in turbot(Scophthalmus maximus),in which the BSA was taken as antigen.In the strip,the rabbit anti-turbot immunoglobulin M,made from this research,labeled with colloidal gold was used as the detector,and the BSA and staphylococcal protein A were blotted on the nitrocellulose membrane for the test and control lines,respectively.The positive serum samples from turbot vaccinated with BSA and negative serum samples from normal turbot were collected to evaluate the characteristics of the strip,and the results were compared with the method of enzyme linked immunosorbent assay(ELISA) established in this study.The strip was shown to be of high specificity and sensitivity,and similar to ELISA.Furthermore,this dipstick assay based on the strip is rapid(5 min) and easy to perform with no requirement of professional skills,reagents or equipments.This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis.Based on the model of this strip developed in the study,this pattern of strip could be performed into any antibody detection in aquaculture animals;it provides a rapid and simple test method to validate the efficacy of the vaccine and to detect and monitor the fish disease in time.
【Fund】: 国家鲆鲽类产业技术体系(CARS-50);; 中国博士后科学基金(20100471499);; 山东省博士后创新项目专项资金(200902008);; 中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022011018)
【CateGory Index】: S943
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 QIN Lei1,WANG Yin-geng2,YAN Bin-lun1 1.Key Laboratory for Oceanic Biotechnology of Jiangsu,Huaihai Institute of Technology,Lianyungang 222005,China;2.Key Lab for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China);Advances in Research on Diseases of Turbot Scophthalmus maximus Affected by Microorganisms[J];Fisheries Science;2008-11
2 ZHANG Fu-xian1,2,WANG Xing-long2(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.The Military Veterinary Institute,Academy of Military Medical Sciences of PLA,Changchun 130062,China);Analysis of the Affecting Factors of Immune Colloidal Gold Technique[J];China Animal Husbandry & Veterinary Medicine;2009-05
3 WANG Wei-fang1 LI Qing-mei2 GUO Jun-qing2 LEI Ji-lin1(1Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,266071)(2Henan Key Laboratory for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002);Progress and prospect of colloidal gold-based immunochromatographic lateral flow assay and its application in aquaculture[J];Progress in Fishery Sciences;2010-03
4 WEI Jian-Teng1,CHEN Ji-Xiang1,WANG Shu-Xian1,ZHANG Xiao-Hua1,WANG Yin-Geng2(1.College of Marine Biology,Ocean University of China,Qingdao 266003,China;2.Yellow Sea Fishery Reseach Institute,CAFS,Qingdao 266071,China);Purification and Application of Serum IgM from Turbot (Scophthalmus maximus)[J];Periodical of Ocean University of China;2008-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Peng,DU Chun-hong,GUO Ying,YING Jia-xiang,SHI Li-yuan,CHEN Ping,TANG Xue,ZHONG You-hong,HUANG Peng,WU Ming-shou,DONG Xing-qi,YU Guo-lin(Yunnan Institute for Endemic Diseases Control and Prevention,Dali,Yunnan 671000,China);Gold Immunochromatographic Assay for a Rapid Diagnosis for Yersinia pestis[J];Endemic Diseases Bulletin;2006-01
2 LI Ke-mei,WANG Yan,WANG Guo-zhi (National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050);Evaluation on the effect of colloidal gold Immunochromatography rapid screen test for detection of brucella infection[J];Chinese Journal of Control of Endemic Diseases;2006-03
3 TANG Yu-de,ZHOU Dong-ming(Huadong Research Institute for Medicine and Biotechnics, Nanjing, Jiangsu,210002,China);The Detection of the Circulating Antigen(CA) of Taenia solium Gysticercosis with Gold Immunochromatographic Assay (GIGA)[J];Progress In Veterinary Medicine;2004-05
4 He Yanling,Lin Song,Wang Ludi,et al.Chinese Academy of Inspection and Quaratine,Beijing 100025,China;Development and Improvement of Colloid Gold Immunochromatography for Rapid Detection of Vibrio Cholerae O_1 Serotype in Aquatic Products[J];Chinese Journal of Frontier Health and Quarantine;2007-01
5 ZHANG Zheng~(1), WANG Yin-geng~(2), HAN Wen-jun~(2),LI Qiu-fen~(2)(1. College of Marine Life, Ocean University of China,Qingdao 266003, China;2. Yellow Sea Fishery Research Institute of Chinese Academy of Fishery Sciences,Qingdao 266071, China);Identification and Phylogenetic Study of Pathogenic BacteriaCausing Ulcerative Fin of Cultured Turbot Scophthalmus maximus[J];Advances in Marine Science;2004-02
6 WANG Xian-lei, ZHANG Pei-jun, LI Jun1 , XU Yong-li (Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,China);Isolation and detection of Japanese flounder mmunoglobulin M[J];Marine Sciences;2004-04
7 Huang Yong li,Cheng Wei xing,Xing Yan chao. (Department of transfusion, PLA Wulumugi General Hospital of Lanzhou Military Area,Wulumugi 830000,China);The Quality Analysis and Application of Dot-immuogold Filtration Assay for HBsAg[J];Journal of Clinical Medical Officer;2003-03
8 ZHANG Jian she, ZHOU Li, XING Jing, ZHAN Wen bin (The Key Laboratory of Miriculture, Ministry of Education, Ocean University of China, Qingdao 266003, China);Preparation of Vibrio parahaemolyticus Lipopolysaccharide and Immunological Analysis of the Lipopolysaccharide AgainstParalichthys olivaceus[J];Journal of Ocean University of Qingdao;2004-04
9 LI Qiang,ZHAN Wen-Bin,SHENG Xiu-Zhen,XING Jing,ZHOU Li(The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China);Detection of Specific Antibody Against Lymphocystis Disease Virus in Japanese Flounder(Paralichthys olivaceus) by Using Monoclonal Antibody Technology[J];Periodical of Ocean University of China;2007-02
10 FENG Juan1,2,HU ChaoQun1(1.South China Sea Institute of Oceanology,CAS,Guangzhou 510301,China;2.South China Sea Fisheries Research Institute,CAFS,Guangzhou 510300,China);PURIFICATION AND CHARACTERISTICS OF SERUM IMMUNOGLOBULINS OF FOUR MAJOR CULTURED MARINE FISHES IN CHINA[J];Tropic Oceanology;2002-04
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