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《Engineering Sciences》 2012-02
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A quick detection method for the early stage of disease in turbot (Scophthalmus maximus)——Development of the colloidal gold-based immunochromatographic strip

Wang Weifang1,Chai Shujun2,Liu Qingtang2,Lei Jilin1, Ding Fuhong1,Hong Lei1,Liu Xinfu1,Su Ke3(1.Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Henan Key Laboratory for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002, China;3.Qingdao General Aquaculture Co.,Ltd.,Qingdao 266404,China)  
In this study,an immunochromatographic strip was developed for the serological detection of antibodies against bovine serum albumin(BSA) in turbot(Scophthalmus maximus),in which the BSA was taken as antigen.In the strip,the rabbit anti-turbot immunoglobulin M,made from this research,labeled with colloidal gold was used as the detector,and the BSA and staphylococcal protein A were blotted on the nitrocellulose membrane for the test and control lines,respectively.The positive serum samples from turbot vaccinated with BSA and negative serum samples from normal turbot were collected to evaluate the characteristics of the strip,and the results were compared with the method of enzyme linked immunosorbent assay(ELISA) established in this study.The strip was shown to be of high specificity and sensitivity,and similar to ELISA.Furthermore,this dipstick assay based on the strip is rapid(5 min) and easy to perform with no requirement of professional skills,reagents or equipments.This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis.Based on the model of this strip developed in the study,this pattern of strip could be performed into any antibody detection in aquaculture animals;it provides a rapid and simple test method to validate the efficacy of the vaccine and to detect and monitor the fish disease in time.
【Fund】: 国家鲆鲽类产业技术体系(CARS-50);; 中国博士后科学基金(20100471499);; 山东省博士后创新项目专项资金(200902008);; 中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022011018)
【CateGory Index】: S943
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Chinese Journal Full-text Database 4 Hits
1 QIN Lei1,WANG Yin-geng2,YAN Bin-lun1 1.Key Laboratory for Oceanic Biotechnology of Jiangsu,Huaihai Institute of Technology,Lianyungang 222005,China;2.Key Lab for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China);Advances in Research on Diseases of Turbot Scophthalmus maximus Affected by Microorganisms[J];Fisheries Science;2008-11
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