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《Guangdong Chemical Industry》 2011-10
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Trace Quantities of Whole Blood on the Experimental Method of the Comet Assay

Xu Haijuan1,Feng Benxiu1,Lin Jian2(1.Guangdong Prevention & Treatment Center for Occupational Diseases,Guangzhou 510310; 2.School of Public Health,Shanxi Medical University,Taiyuan 030001,China)  
Objective: The problem of experimental method was discussed with whole blood and lymphocytes of rat by comet assay.Methods: Tail blood was collected to test by comet assay after 24 h intraperitoneal injection.Normal saline treated group used as the negative control.Cyclophosphamide treated group used as the positive control as treated(40 μg/kg).Results: There was no marked difference between the result of blood comet and lymphocytes comet(P0.05).With the rising of electrophoresis voltage and pyrolysis time,comet tail length increased.And there is no marked difference in result read on the same day and saved by ethanol dehydration.Conclusion: The results indicated that comet assay with trace quantities of whole blood can be used in screening.
【CateGory Index】: R114
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