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Serial paraffin section and hematoxylin-eosin staining of the Amphioxus

LEI Jia-yao,XU Ye,GU Huai-yu.Department of Anatomy,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,510080,China  
Objective To introduce a method for serial paraffin section and hematoxylin-eosin staining of the Amphioxus Methods Leica EG1150 Modular Tissue Embedding Center(consisting of the Leica EG1150 C Cold Plate and the Leica EG1150 H Heated Paraffin Dispensing Module),Leica VT1000S Vibrating Blade Microtome,Leica HI1210-Waterbath,Leica HI1220-Flattening Table were utilized to produce the serial paraffin sections as well as obtaining hematoxylin-eosin staining of the Amphioxus.By comparing the appearance changes and microscopic images of the Amphioxus repetitively step by step,we found out the appropriate time course of every procedure.Results We attained clear hematoxylin-eosin stained paraffin sections in that way.The sections of head,thorax,abdomen and tail were selected as the four representatives,the structures of notochord,neural tube,pharyngeal gill,ovary,myomere,intestine of the Amphioxus were clearly distinguished.Conclusion We established a successful method for serial paraffin section and hematoxylin-eosin staining of Amphioxus,and provided a new way for the further research in origin of vertebrates.
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