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《Guangdong Agricultural Science》 2005-01
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Studies on the determination of balsam pear F_1 hybrid by RAPD technique

SUN Ni~1, HU Kai-lin~(1*), ZHANG Chang-yuan~2 (1.College of Horticulture, South China Agricultural University, Guangzhou 510642,China; 2.Institute of Vegetable Research, Guangdong Academy of Agricultural Science, Guangzhou 510640,China)  
RAPD (random amplified polymorphic DNA) analysis was employed in order to study the specific fragment in balsam pear (Momordica charantia L.) hybrid F_1'DingYou'and it's parents. The results showed that primer OPU19 amplified one OPU19-1600 bp fragment which appeared in F_1 and male parent, but not in the female parent. The analysis of F_2 and backcrosses progeny revealed that this fragment might be linked with one gene of male parent, and it conform to the Mendelian inheritance of one pair of dominant gene. Thus OPU19-1600 bp could be used to test the seeds of F_1 hybrid and inbred of female parent.
【Fund】: 广东省农业攻关项目(2002A2070101)
【CateGory Index】: S642.5.037
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