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《Guangdong Agricultural Sciences》 2008-05
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Extraction of Cotton Genomic DNA Using Development SDS

LU Dong-bai1,2,LI Xiao-fang1,LIU Zhi-xia1(1.Rice Research Institute of Guangdong Academy of Agricultural Sciences /Guangdong Province Key Lab of New Technology In Rice Breeding,Guangzhou 510640,China;2.Agriculture college of Yangtze University,Jingzhou 434025,China)  
This research reported a development SDS method for extraction cotton genomic DNA.Through DNA extraction of general all purpose SDS method at present,we added anti-oxidant(polyvinylpyrrolidone 40 and β-mercaptoethanol)to exclude polyphenol and other secondary interfacing substance.It showed that development SDS method can solve quality of cotton DNA extraction and browning easily.
【Fund】: 广东省自然科学基金项目(06200585 04101155);; 农业部“948”项目(2006-G1)
【CateGory Index】: S562
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