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Preliminary study on SSR makers linked to the resistance gene of MRDV in maize

HE Long1,WANG Bo-chu1,WU Hong2,LEI Kai-rong2(1.Bioengineering College of Chongqing University,Chongqing 400030,China; 2.Biotechnology Research Center,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China)  
In this paper,189 F2 individuals from the cross between the resistant maize(Qi 319) and the susceptible maize(Ye 478) were exploited to detect the SSR molecular markers linked with gene of MRDV.88 pairs of primers were screened by BSA,only Bnlg125 primer and Bnlg1064 primer showed polymorphic in resistant and susceptible DNA pools,among which a polymorphic fragment(named Bnlg125-410) about 410 bp was amplified by Bnlg125 primer.When calculated by Mapmaker/Exp software,it was found that the genetic distance between the locus of MRDV resistance and Bnlg125-410 marker was 5.8 cM.
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