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《Guangdong Agricultural Sciences》 2011-19
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Cloning and sequence analysis of Cu/Zn superoxide dismutase gene 2(MrSOD2) cDNA from Myrica rubra

DONG Le,WANG Fang,DAI Cong-jie,LIN Luan,CHEN Duan-yu,WANG Yu-yuan,LIN Jing(School of Chemistry and Life Science,Quanzhou Normal College,Quanzhou 362000,China)  
A PCR-based homologous cloning strategy was used to identify superoxide dismutase(SOD) genes from the leaves of Myrica rubra.Results of sequence analysis indicated that a 233 bp cDNA with a conserved regions encoding a 77 amino acids actin ortholog was successfully cloned and characterized.The SOD contained the signature sequence of Cu/Zn-SOD gene family.Multiple alignment analysis of amino acid sequences of the putative SOD conserved regions in Myrica rubra was more than 86% homologous with the best scoring results from similarity searches of sequence databanks.This gene was named as MrSOD2(Genbank accession No.AB 661320).
【Fund】: 福建省教育厅科技计划项目(JA09212);; 泉州市优秀人才培养专项(09A07);; 福建省高校服务海西建设重点项目(A101);; 福建省大学生创新性实验计划项目(2010CXSY16)
【CateGory Index】: S667.6
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