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Study on microbial fermentation of distiller

GUO Su-huan1,ZHOU Bi-jun1,2,LIU Xin1,LI Xiao-dan1,WEN Ming1,2, CHENG Zhen-tao1,2,WANG Kai-gong1,2,XIA Xian-lin1,2 (1.College of Animal Science,Guizhou University,Guiyang 550025,China; 2.Key Laboratory for Amimal Epidemic Disease of Guizhou,Guiyang 550025,China)  
The experiment was conducted to get the optimum conditions and combination of the Brettanomyces custersianus,Bacillus subtilis(Ehrenberg) Cohn,Trichoderma viride and Lactobacillus acidophilus for fermentation distiller by using the antagonistic test of cross method under the anaerobic and aerobic fermentation condition.The growth of Trichoderma viride was observed by the single factor experiment.The results showed that there were no antagonist responses between the four kinds of bacteria.The number of living bacteria getting from the aerobic fermentation was higher than anaerobic fermentation,and it could get the highest number on the ninth day for Lactobacillus acidophilus and Brettanomyces custersianus fermentation.The aerobic fermentation group which the combination was Lactobacillus acidophilus with Brettanomyces custersianus and Trichoderma virides got the best result for Lactobacillus acidophilus,which was an order of magnitude higher than the anaerobic fermentation.The aerobic fermentation group which the combination was Lactobacillus acidophilus with Brettanomyces custersianus got the best result for Brettanomyces custersianus,Which was 47.95% higher than the anaerobic fermentation.Each group of Bacillus subtilis(Ehrenberg) Cohn fermentation got the highest number of living bacteria on the sixth day,and the aerobic fermentation group which the combination was Lactobacillus acidophilus with Bacillus subtilis(Ehrenberg) Cohn got the highest number,which was 11.73% higher than anaerobic fermentation.The number of living bacteria for Trichoderma viride was all zero.And we found that Trichoderma viride was not suitable for the fermentation of distiller by the the single factor experiment.
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