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《Guangdong Agricultural Sciences》 2012-13
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Expression analysis of MKK4 gene in Penaeus monodon

SUN Wen-wen1,2,YANG Li-shi1,SU Tian-feng1,YANG Qi-bin1,ZHU Cai-yan1,ZHOU Fa-lin1(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture,Guangzhou 510300,China;2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)  
The Penaeus monodon MKK4 cDNA was isolated from an ovary cDNA library.To study the biological functions of MKK4 in P.monodon,we compared the expression level of MKK4 in different tissues of female and male with real-time quantitative PCR.The results showed that the MKK4 mRNA was ubiquitously distributed in various tissues of P.monodon,with the highest levels in testis.Furthermore,the MKK4 expression was found to be a higher level in immature testis than in mature testis.The MKK4 expression was found in the six ovarian stages of development,and the expression levels rose gradually from the second growth phase to sixth growth phase,with the highest levels in the sixth growth phase.The study provided essential materials for the further research of the function of
【Fund】: 国家自然科学基金(40976101);; 中央级公益性科研院所基本科研业务费(2012TS04);; 广东省海洋与渔业科技推广专项(A201001B01);; 国家虾产业技术体系专项(CARS-47);; 海南省重点科技计划项目(ZDXM20110016)
【CateGory Index】: S917.4
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