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《Guangdong Agricultural Sciences》 2013-05
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Cloning and sequence analysis of HbERF3 promoter from Hevea brasiliensis

YU Xiao-hui1,2,ZHU Jia-hong2,XU Jing3,CHANG Wen-jun2,ZHANG Zhi-li2,3(1.College of Horticulture and Garden,Hainan University,Haikou 570228,China;2.Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;3.Hainan Academy of Agricultural Sciences,Haikou 571000,China)  
This experiment was designed to study the expression regulation,function in signal transduction and the stress-resistion of HbERF3 gene in Hevea brasiliensis.We designed two specific primers based on the sequence of the ERF3 gene from H.brasiliensis,and a 1 572 bp fragment was cloned from the genomic DNA of H.brasiliensis by GenomeWalking method.Sequence analysis showed that the fragment was the 5’ upstream promoter region of ERF3 gene and it contained typical eukaryotic core promoters and a transcription start site.The analysis of the cis-acting elements showed that the fragment contained some TATA-box,CAAT-box as well as some elements that responsed to hormone and stresses.The cloning and the sequence analysis of the HbERF3 promoter lay the foundation to a further study of the regulation and function of HbERF3.
【Fund】: 国家自然科学基金(31000313);; 海南省自然科学基金(309052);; 中央级公益性科研院所业务费专项(ITBB110201)
【CateGory Index】: S794.1
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