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《Guangdong Agricultural Sciences》 2013-11
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Application of PCR-DGGE to analysis the microbial community diversity in biological biofilm reactor

YI Lv-yun,LI Hong-yang,SONG Shan-ke,PU Yue-wu(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China)  
In order to reveal microbial community structure diversity of bioflim reactor processing simulation domestic wastewater,the start-up initial stage and operation later stage biofilm from porcelain plate sliding surface,porcelain plate coarse surface,white rubber sheet and black experiment countertops were taken and the genomic DNA of microbial communities were extracted by the method of cell lysis.PCR-amplified 16S rRNA which using the universal primers and denaturing gradient gel electrophoresis(DGGE) were applied to obtain the DNA fingerprint profile of the microbial communities.The results showed that similarity between microbial communities of different blocking material were up to 78.3%,the lowest of 41.4%,the profile of DGGE showed that different biofilm samples had both different microbial species and common species.However,the biofilm community structure of both start-up initial stage and operation later stage are maintaining stability and the succession was obvious.In addition,part of the biofilm microbial community of dominant bacteria was analyzed through cloning sequencing and phylogenetic analysis.Five bacterial 16S rDNA sequences were identified and with a homology over than 97% when compared with the bacteria of Clostridia bacterium sp.,Clostridium sp.and Brevundimonas sp..These dominant microorganisms played important roles in the removal of organic pollutants in the bioflim system.
【CateGory Index】: X703
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Chinese Journal Full-text Database 7 Hits
1 Abd El-Latif Hesham1,2, Rong Qi1, MinYang1,* 1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. 2. Genetics Department, Faculty of Agriculture, Assiut University, Assiut, Egypt;Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis[J];环境科学学报(英文版);2011-12
2 MOU Jie1,SUN Baosheng1,CHEN Yi1,21.School of Environmental Science and Technology,Tianjin University,Tianjin 3000722.Sinopec Ningbo Engineering Company Limited,Ningbo 315103;Microbial community structure in a membrane bioreactor determined using PCR-DGGE[J];Acta Scientiae Circumstantiae;2010-04
3 Chi Juan,Zhang Guo-zhao,Li Min-zhe,Zhan Lan,Lu Gang,Lan Zhao-kun(Eco-Environmental Research Center,Chinese Academy of Sciences,Beijing 102200,China);EXPERIMENTAL RESEARCH ON DOMESTIC SEWAGE TREATMENT BY MBR AND PROJECT DESIGN[J];Technology of Water Treatment;2005-10
4 Yang Jian-zhong1,Liu Yi-hui1,Zhang Xing-wen2,Yang Feng-lin1,2,Kenji Furukawa3(1.School of Chemical Engineering,Dalian University of Technology,Dalian 116012,China;2.School of Environmental and Biological Science and Technology,Dalian University of Technology,Dalian 116023,China;3.Faculty of Engineering,Kumamoto University,Kumamoto,Japan);DOMESTIC WASTEWATER TREATMENT BY BIOFILM REACTOR[J];Technology of Water Treatment;2005-11
5 DONG Bin,LIANG Ya,ZHOU Zengyan,GAO Tingyao(School of Environmental Science and Engineering,Tongji University,Shanghai 200092,China);Influence of High Organic Load on Nitrogen and Phosphorus Removal in an Activated Sludge-Bilfilm Combined System[J];Journal of Tongji University(Natural Science);2006-08
6 Wang Qizan Xu Qiufang Jiang Peikun Qin Hua(School of Environmental and Science and Technology,Joint Lab. of Forest Soils and Environment,Zhejiang Forestry University & ISSCAS, Zhejiang Forestry University,Lin’an,Zhejiang 311300,China);DGGE ANALYSIS OF PCR OF 16S rDNA V3 FRAGMENTS OF SOIL BACTERIA COMMUNITY IN SOIL UNDER NATURAL BROADLEAF FOREST INVADED BY PHYLLOSTACHY PUBESCENS IN TIANMU MOUNTAIN NATURE RESERVE[J];Acta Pedologica Sinica;2009-04
7 Meng Rong Pu Yuewu Ren Dunjian Yi Lüyun (School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006);Application of PCR-DGGE to Analysis of Microbial Community Diversity in Dyeing Wastewater[J];Biotechnology Bulletin;2012-10
Chinese Journal Full-text Database 10 Hits
1 SUN Xiao-qin et al (School of Life Science,Nanchang University,Nanchang,Jiangxi 330031);Optimization of ISSR-PCR Reaction Conditions for Genomic DNA from Cymbidium kanran Makino[J];Journal of Anhui Agricultural Sciences;2009-29
2 Zhang Pu1,Fu Xinghua1,Wang Junqiang1,Wu Cinan1,Liu Zewen2(1.School of Science,Guizhou University,Guiyang 550025,China; 2.Institute of Microelectronics,Tsinghua University,Beijing 100084,China);Research on Micro-Pt Temperature Sensor and Its Read-Out Circuit[J];Semiconductor Technology;2012-06
3 WANG Wen-li,TAN Jing,ZHENG Run-sheng,XU Hui(Research Centre of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine/Key Laboratory of Chinese Medicine Resources of Ministry of Education,Guangzhou 510006,China);Research advance on molecular identification of mycotoxigenic fungi[J];Guangdong Agricultural Sciences;2012-10
4 ZHU Liang1,ZHU Feng-chun1,XU Xu-chang2,XU Wei-xin1,WANG Zhan-sheng3, GU Jun-nong4(1.College of Environmental Science and Engineering,Hohai University,Nanjing 210024,China; 2.Nantong Urban Planning and Design Co.Ltd.,Nantong 226000,China;3.Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China;4.Beijing Waterworks Group,Beijing 100038,China);Combined Process of MBR and PAC for Treatment of Tailwater from WWTP[J];China Water & Wastewater;2009-05
5 GAO Jun-wei1,GUO Yan-feng1,WANG San-hu1,2,PAN Yao-qian1,2, WANG Li-rong1,2,ZHU Guo-po1,LIU Xing-you1,2 (1.School of Animal Science of Henan Institute of Science and Technology,Xinxiang 453003,China;2.Henan Higher Education Engineering Technology Research Centre for Animal Diseases Control and Residues Supervision,Xinxiang 453003,China);The Principle and Application of Fluorescent Quantitation PCR to Research the Distribution Regulation of Virus in Animal Body[J];China Animal Husbandry & Veterinary Medicine;2010-04
6 SHI Yu-long1,WANG San-fan1*,CHEN Xia2(1.Engineering Research Centre of Water Resource Utilization in Cold and Drought Region of Ministry of Education,School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China; 2.School of Architecture and Civil Engineering,Liaocheng University,Liaocheng 252059,China);Biological Phosphorus and Nitrogen Removal in Low C/N Ratio Domestic Sewage Treatment by Velocity Discrete Biofilter[J];Environmental Science & Technology;2012-11
7 SHI Xiao-meng,TAN Fei-yan,HUANG Shou-xian * ,MENG Yi-yi,HUANG Jia-hua (Forestry College of Guangxi University,Key Laboratory of Fast-growing Tree Breeding in Central South China of State Forestry Administration,Nanning 530005,China);Optimization of ISSR reaction system of Liriodendron chinese[J];Journal of Southern Agriculture;2013-07
8 Yu Zongling;Wang Rui;Tian Yuzhe;Key Laboratory of Plant Biology,College of Heilongjiang Province;College of Life Science and Technology,Harbin Normal University;;PCR-DGGE Technique in Biological Wastewater Treatment Application[J];Environmental Science and Management;2013-09
9 PENG Hui-Qing,YU Sheng-ying,ZHAO Huan(School of Resource and Environmental Engineering,Wuhan University of Technology,Wuhan,Hubei 430070);Application of PCR Technology to the Determination in Environment Microorganism[J];Resources Environment & Engineering;2007-05
10 HAN Ye,ZHOU Zhi-jiang,WANG Pei-pei,ZHANG Li-xia(College of Agriculture and Bioengineering,Tianjin University,Tianjin 300072,China);Cloning and Expression of Pediocin Gene[J];Acta Agriculturae Boreali-Sinica;2009-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 YU Xin1,LI Xudong2,YANG Junshi2,ZHANG Xiaojian1(1.Department of Environmental Science and Engineering, Tsinghua University, Beijing100084,China;2.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu610041,China);Biofilm Morphology and Activity in Biopretreatment for Polluted Raw Water[J];Urban Environment & Urban Ecology;2003-01
2 MAJianyong1,ZHANGXingwen1,YANGFenglin1,ZHANGHanmin1,LIUYihui1,KENJIFurukawa2( 1.School of Environ. Sci. and Technol., Dalian Univ. of Technol., Dalian 116012, China;2.Dept. of Civil Eng.& Archit., Kumamoto Univ., Kumamoto, Japan );Treating low concentration wastewater by moving bed biofilm reactor[J];Journal of Dalian University of Technology;2003-01
3 Jiang Xin1,3,Ma Mingchao2,Li Jun3,Lu Anhuai1,Zhong Zuoshen41.School of Earth and Space Sciences,Peking University,Beijing 100871,China2.Beijing Municipal Research Institute of Environmental Protection,Beijing 100037,China3.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agriculture Science,Beijing 100081,China4.School of Water Resources and Environment,China University of Geosciences(Beijing),Beijing 100083,China;Analysis on the bacterial diversity of active sludge in wastewater treatment plant.[J];Earth Science Frontiers;2008-06
4 ZHANG Bo 1, GAO Ting yao 2 (1.Depart.of Environ.Eng.,Qingdao Construction Institute,Qingdao 266033, China;2. State Key Lab.of Pollution Control and Resource Reuse,Tongji Univ.,Shanghai 200092, China);Principle and Characteristics of Reversed A~2/O Process[J];CHINA WATER & WASTEWATER;2000-07
5 WANG Rong1,2,WANG Hua1,WAN Jin-bao2(1.College of Land Resources and Environment,Jiangxi Agriculture University,Nanchang 330045,China;2.Key Lab of Poyang Lake Ecology and Bio-resource Utilization <Ministry of Education>,Nanchang University,Nanchang 330029,China);Control of Filamentous Sludge Bulking during Sludge Acclimation in MBR[J];China Water & Wastewater;2009-03
6 Zhou Xiaofei1,2,Liu Huicheng3,Chen Hang3,Hong Huasheng1(1.Environmental Science Research Centre,Xiamen University,Xiamen 361005,China;2.Department of Environment Sciences,South China University of Tropical Agriculture,Danzhou 571737,China;3.Shunhuan Municipal Engineering Equipment Co.,Ltd.,Foshan 528300,China);Treatment of wastewater from jeans production by hydrolytic acidification/biological contact oxidation/sedimentation[J];Industrial Water Treatment;2007-05
7 Wu Xiaoliang,Jiang Shuangying,Gao Tingyao (School of Environmental Science and Engineering,Tongji University,Shanghai 200092,China);Treatment of Dyeing Wastewater by Coagulation-Hydrolysis Acidification-Contact Oxidation-Air Flotation Process[J];Environmental Protection of Chemical Industry;2009-03
8 Chen Yi1,2 Sun Baosheng1 Huang Xing1 Zhang Bin1(1.School of Environmental Science and Engineering,Tianjin University,Tianjin 300072,China;2.Sinopec Ningbo Engineering Company Limited,Ningbo 315103,China);Study of microbial population succession in MBR at different stages[J];Chinese Journal of Environmental Engineering;2009-06
9 LIU Xin-chun, ZHANG Yu, YANG Min, WANG Zhen-yu, LV Wen-zhou State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.;Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method[J];环境科学学报(英文版);2007-01
10 DUAN Xue jun 1,2 ,MIN Hang 1 (1. College of Life Science, Zhejiang University, Hangzhou 310029,China; 2. Zhongyuan Institute of Technology, Zhengzhou 450007,China);Diversity of Microbial Genes in Paddy Soil Stressed by Cadmium Using DGGE[J];Environmental Science;2004-05
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