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《Guangdong Agricultural Sciences》 2013-14
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Screening selective reagents for genetic transformation in Haematococcus plauvialis

FAN Qing-de1,ZHANG Xue-qin2,WANG Chao-gang2(1.School of Life Science and Chemistry,Hulunbiur College,Hulunbiur 021008,China;2.College of Life Sciences,Shenzhen University,Shenzhen 518060,China)  
Haematococcus plauvialis 34-1n and 192.80 were employed in this research.In order to analyze their growth characters,counting-cell-method and microscope were used.After adding different concentration of zeomycin,phleomycin and paromomycin to BBM medium,cells were counted and observed using microscope.Results showed that H.plauvialis 31-1n was more suitable for genetic transformation than H.plauvialis 192.80.Further studies also indicated that H.plauvialis 31-1n was highly sensitive to zeomycin,while not sensitive to paromomycin.The concentration of 8 μg/mL zeomycin might be a suitable concentration when building up transformation system in H.plauvialis.Hence,it suggested that zeomycin could be suitable agent and ble gene could be a selectable marker for genetic transformation in H.plauvialis.
【Fund】: 国家自然科学基金(31000162)
【CateGory Index】: S968.43
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