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《Journal of Hepatobiliary Surgery》 2013-02
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Construction and Identification of Eukaryotic Expression Vector pcDNA3.1(+)-ABCG2

(LUO Wei-huan1,JIANG Chun-ping2,DING Yi-tao2 1.Nanjing University Medical College Nanjing,Jiangsu 210008,China;2.Department of Hepatobiliary Surgery,Affi liated Drum Tower Hospital of Nanjing University,Nanjing 210008,China)  
Objective To construct the human ABCG2 gene eukaryotic expression vector pcDNA3.1(+)-ABCG2,and express in The ABCG2 negative SMMC7721 of hepatoma cells.Method RNA extracted from human cancer cells,via reverse transcription cDNA by PCR,primers were designed based on the sequence information of the human ABCG2 gene 62526032(NM_004827.2),CDS sequences of ABCG2 gene were amplified and cloned into pcDNA3.1 + expression vector.The recombinant eukaryotic plasmid pcDNA3.1(+)-ABCG2 was transfected into ABCG2 negative SMMC7721 of hepatoma cells by Lipofectin.Western blot was used to certify whether the gene was correctly expressed in The ABCG2 negative SMMC7721 of hepatoma cells.Results The amplified fragment by PCR was coincident with the anticipated result,and its sequence was in concordance with the theoretical fragment size.Therefore,the ABCG2 gene was cloned successfully,and the recombinant plasmid pcDNA3.1(+)-ABCG2 was also constructed successfully.On the protein level,the expression of ABCG2 gene was increased obviously in the transfected cells detected by Western blot.Conclusion We successfully constructed recombinant eukaryotic expression vector pcDNA3.1(+)-ABCG2,and it could be obviously expressed in negative ABCG2 SMMC7721 hepatoma carcinoma cells.
【Fund】: 江苏省南京市卫生局科技发展基金资助项目(项目编号:ZKX08025);; 卫生部科研课题(江苏省)(项目编号:LW201008)
【CateGory Index】: R735.7
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 Zhong Xi,Chun-Ping Jiang,Yi-Tao Ding,Department of Hepatobiliary Surgery,Affiliated Drum Tower Hospital,Nanjing Medical University,Nanjing 210008,Jiangsu Province,China;Expression of stem cell marker ABCG2 and its signifi cance in hepatocellular carcinoma tissue and cell lines[J];World Chinese Journal of Digestology;2009-03
2 Kai Song,Shuo-Fei Yang,Jun-Hua Wu,School of Medicine,Nanjing University,Nanjing 210046,Jiangsu Province,China Kai Song,ShuoFei Yang,Chun-Ping Jiang,Institute of Hepatobiliary Surgery,Nanjing University,Nanjing 210018,Jiangsu Province,China Chun-Ping Jiang,Department of Hepatobiliary Surgery,the Affiliated DrumTower Hospital of Nanjing University Medical School,Nanjing 210018,Jiangsu Province,China;Advances in research on liver cancer stem cells[J];World Chinese Journal of Digestology;2009-36
3 Guang-Hui Qiang,De-Cai Yu,Chun-Ping Jiang,Department of Hepatobiliary Surgery,the Affi liated Drum Tower Hospital of Nanjing Medical University,Nanjing 210008,Jiangsu Province,China ,Department of Hepatobiliary Surgery,the Affi liated Drum Tower Hospital of Nanjing University,Nanjing 210008,Jiangsu Province,China;Side population cells and liver cancer stem cells[J];World Chinese Journal of Digestology;2010-10
4 QIANG Guang-hui1,YU De-cai2,DING Xi-wei3,JIANG Chun-ping2.1.Department of General Surgery,Gaochun People's Hospital,Nanjing 211300,Jiangsu Province,China;2.Department of Hepatobiliary Surgery,Affiliated Drum Tower Hospital of Nanjing University Medical College,Nanjing 210008,Jiangsu Province,China;3.Nanjing University Medical College,Nanjing 210008,Jiangsu Province,China;Expression of ABCG2 in Human Liver Cancer Cell Lines and Its Related Functions[J];Chinese Journal of Bases and Clinics in General Surgery;2012-02
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 CHENG Hao,JIA Xi-hua,ZHANG Xiao-wei,ZHANG Jin-ku First Central Hospital of Baoding,Baoding 071000,P.R.China;Expressions and clinical significance of ABCG2 and P-gp protein in gastric carcinoma tissues[J];Chinese Journal of Cancer Prevention and Treatment;2013-04
2 Kai Song,Shuo-Fei Yang,Jun-Hua Wu,School of Medicine,Nanjing University,Nanjing 210046,Jiangsu Province,China Kai Song,ShuoFei Yang,Chun-Ping Jiang,Institute of Hepatobiliary Surgery,Nanjing University,Nanjing 210018,Jiangsu Province,China Chun-Ping Jiang,Department of Hepatobiliary Surgery,the Affiliated DrumTower Hospital of Nanjing University Medical School,Nanjing 210018,Jiangsu Province,China;Advances in research on liver cancer stem cells[J];World Chinese Journal of Digestology;2009-36
3 Bo Wang,Yu-Jia Xia,Wei Yan,Li-Min Xia,Mei Liu,DeAn Tian,Department of Gastroenterology,Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China;Regulation of side population cells by TGF-β1-induced epithelial-mesenchymal transition in hepatocellular carcinoma cell lines SMMC-7721 and HepG2[J];World Chinese Journal of Digestology;2010-23
4 HUO Xiao-kui,LIU Ke-xin Department of Clinical Pharmacology,College of Pharmacy,Dalian Medical University,Dalian 116044,China;Advances in studies on ATP-binding cassette transporter-mediated multidrug resistance in liver cancer[J];Drug Evaluation Research;2012-01
5 ZHENG Qiu-hong1,XU Yang-mei1,WEI Zhi-qiang1,GONG Fu-sheng1,YANG Jian-wei2,XIE Yun-qing1,YING Min-gang3(1.Department of Molecular Biology of Tumor,Affiliated Teaching Hospital of Fujian Medical University,Tumor Hospital of Fujian Province,Fuzhou 350014,Fujian,China;2.Department of Internal Oncology,Affiliated Teaching Hospital of Fujian Medical University,Tumor Hospital of Fujian Province,Fuzhou 350014,Fujian,China;3.Department of Surgical Oncology,Affiliated Teaching Hospital of Fujian Medical University,Tumor Hospital of Fujian Province,Fuzhou 350014,Fujian,China);Isolation of stem-like cells from human hepatocellular carcinoma PLC/PRF-5 cells and screening for their specific miRNAs[J];Chinese Journal of Cancer Biotherapy;2011-02
6 Ai-Jie Zhang, Ke-Xin Liu, Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, Liaoning Province, China;Drug-drug interactions mediated by hepatic transporters[J];World Chinese Journal of Digestology;2012-28
7 HUANG Qin-xian;LI Hai-yang;HU Yi;SONG Jian-ning;DONG Biao;CAO Kun;Department of Hepatobiliary Surgery,Affiliated Hospital of Guiyang Medical University;;Experimental Study on Establishment of Cell Proliferation Model and Isolated Method in Vitro of Hepatic Oval Cells in Adult Rat[J];Chinese Journal of Bases and Clinics in General Surgery;2013-10
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 WANG Ge~ 1 ,SUO Jin-you~ 1,2 , DENG Jing~ 1 ,YANG Jin~ 1 ,ZHENG Ji-jun~ 1 , WANG Hong-zhong~ 1 , HU Qing~ 1 , LI Zeng-peng~ 3 , XIAO Hua-liang~3, WANG Dong~ 1,3 (~ 1 Cancer Center, ~ 2 Department of Hepatobiliary Surgery, ~ 3 Department of Pathology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China);Origin of hepatic stem cells in human hepatocellular carcinoma[J];Acta Academiae Medicinae Militaris Tertiae;2006-02
2 YAN Zheng, FANG Chi-hua, GAO Peng Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;Preliminary research of hepatocarcinoma stem cell markers[J];Journal of Southern Medical University;2006-09
3 Monika Olempska, Patricia Alice Eisenach, Ole Ammerpohl, Hendrik Ungefroren, Fred Fandrich and Holger Kalthoff Department of General and Thoracic Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Arnold Heller Str. 7, 24 105 Kiel, Germany;Detection of tumor stem cell markers in pancreatic carcinoma cell lines[J];国际肝胆胰疾病杂志(英文版);2007-01
4 OU Yang,GUO Xiu-Li(Department of Newdrug Pharmacology,School of Pharmaceutical Sciences,Shandong University,Jinan 250012,China);Tumor Stem Cells and Drug Resistance[J];Progress in Physiological Sciences;2007-02
5 Yan Zheng, Fang Chi-Hua, Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China;Elementary study on subpopulation cell isolation and tumor heterogeneity of human hepatocellular carcinoma[J];World Chinese Journal of Digestology;2006-05
6 Xiao-Lei Shi,Liang Mao,Ting Xie,Zhang-Hua Zhu,Yi-Tao Ding,Department of Hepatobiliary Surgery,the Affi li-ated Drum Tower Hospital of Nanjing University Medical College,Nanjing 210008,Jiangsu Province,China Bi-Yun Xu,Jun-Hao Chen,Lei Li,Scientific Research Department,the Affi liated Drum Tower Hospital of Nanjing University Medical College,Nanjing 210008,Jiangsu Pro-vince,China;Optimization of an effective directed differentiation medium for mouse bone marrow mesenchymal stem cells into hepatocytes in vitro[J];World Chinese Journal of Digestology;2008-20
7 Zhong Xi,Chun-Ping Jiang,Yi-Tao Ding,Department of Hepatobiliary Surgery,Affiliated Drum Tower Hospital,Nanjing Medical University,Nanjing 210008,Jiangsu Province,China;Expression of stem cell marker ABCG2 and its signifi cance in hepatocellular carcinoma tissue and cell lines[J];World Chinese Journal of Digestology;2009-03
8 Bao-Rui Liu, Ting-Ting Wang, Xiao-Ping Qian, Oncol-ogy Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Clinical Oncology Institute of Nanjing University, Nanjing 210008, Jiangsu Province, China;Advance in molecular targeted therapy for primary hepatocellular carcinoma[J];World Chinese Journal of Digestology;2009-10
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