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《CHEMICAL RESEARCH IN CHINESE UNIVERSITIES》 1997-05
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Studies on the Mechanism of Fluorescence Quenching of Tb~(3+)-Tiron Complex by Nucleotides, Polynucleotides and Nucleic Acids

ZHAO Yi-Bing; WANG Dong-Yuan; GUO Xiang-Qun; XU Jin-Gou(Department of Chemistry, The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Xiamen University, Xiamen, 361005)  
The mechanism of fluorescence quenching of Tb3+-Trion complex by nucleotides,polynucleotides and nucleic acids has been studied. It was considered that it is a static quenching which is based on the phosphate moieties of nucleotides, polynucleotides and nucleic acids conpeting the Tb3+ ion with Tiron and forming non-fluorescent binary complex under the optimal conditions. The experimental results of nucleic acids reacting with intercalating reagent of DNA or metal ion using Tb3+-Trion complex as a fluorescence probe indicated that the mechanism is reasonable.
【Fund】: 国家教育委员会博士点基金
【CateGory Index】: O614
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