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《CHEMICAL RESEARCH IN CHINESE UNIVERSITIES》 1997-09
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Synthesis of High Efficiency Packing and Using for Recombinant Human Interferons Separation in High-performance Hydrophobic Interaction Chromatography

GUO Li-An(The Centre Laboratory, 4th Military Medical University, Xi'an, 710032)ZHU Bao-Quan(Shanghai institute of Pharmaceutical industry, Shanghai, 200040)  
The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper. It was showed that the packingsynthesized was not only suitable for separating standard proteins, but also for different rhIFNs,i. e., rhIFN-αA expressed in yeast, rhIFN-αA and rh1FN-γ in E. colt. The purify of threerhIFNs purified by one-step HPHIC, which rhIFN-γ expressed in E. colt was recognized as80%, rhIFN-αA expressed in yeast as 70%, and rhIFN-αA expressed in E. colt as 30%, wasmore than that of other chromatography besides affinity chromatography.
【Fund】: 军队医药卫生科研基金
【CateGory Index】: O657.7
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【References】
Chinese Journal Full-text Database 2 Hits
1 GUO Li-An;ZHU Bao-Quan(The Centre Laboratory, Fourth Military MedicalUniversity, Xi'an 710033, China,) ZCHEN Dai--Jie( Shanghai Institute of Pharmaceutical Industry, China);Purification of recombinant human interferon-α by magnetic affinity microsphere[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-01
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Guo Li'an ,Liang Guodong, Sun Ying (Central Laboratory, Tangdu Hospital ,The Fourth Military Medical University, Xi'an 710038)Chang Jianhua (Department of Chemistry, Northwest University);Study on Protein Conformational Changes in Hydrophobic Interaction Chromatography[J];Chinese Journal of Analytical Chemistry;1993-07
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 WANG Yun 1 , WANG Zheng 2 (1.Department of Biochemistry,Changshu College,Changshu 215500; 2.College of Bioscience & Biotechnology,Yangzhou University 225009);Applications of Hydrophobic Interaction Chromatography in Biochemical Research[J];JOURNAL OF CHANGSHU COLLEGE;2000-04
2 Liu Tong and Geng Xindu * (Institute of Modern Separation Science, Northwest University, Xian, 710069);New Development of Hydrophobic Interaction Chromatography and Its Applications in Biochemical Research[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1998-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Yuan-Kun, GUO Li-An, LIANG Guo-Dong and ZHUANG Wen-Xu Central Laboratory, Tangdu Hospital Department of Biology;Preparing magnetite albumin microspheres containing adriamycin[J];Journal of The Fourth Military Medical University;1991-05
2 Guo Li'an ,Liang Guodong, Sun Ying (Central Laboratory, Tangdu Hospital ,The Fourth Military Medical University, Xi'an 710038)Chang Jianhua (Department of Chemistry, Northwest University);Study on Protein Conformational Changes in Hydrophobic Interaction Chromatography[J];Chinese Journal of Analytical Chemistry;1993-07
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【Secondary References】
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